Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17400 and BWI76_RS17405 are separated by 150 nucleotides BWI76_RS17405 and BWI76_RS17410 are separated by 265 nucleotides BWI76_RS17410 and BWI76_RS17415 are separated by 89 nucleotides BWI76_RS17415 and BWI76_RS17420 are separated by 39 nucleotides
BWI76_RS17400: BWI76_RS17400 - transporter, at 3,610,065 to 3,610,745
_RS17400
BWI76_RS17405: BWI76_RS17405 - cation transport regulator, at 3,610,896 to 3,611,126
_RS17405
BWI76_RS17410: BWI76_RS17410 - sodium-potassium/proton antiporter ChaA, at 3,611,392 to 3,612,492
_RS17410
BWI76_RS17415: BWI76_RS17415 - 3-deoxy-8-phosphooctulonate synthase, at 3,612,582 to 3,613,436
_RS17415
BWI76_RS17420: BWI76_RS17420 - hypothetical protein, at 3,613,476 to 3,614,288
_RS17420
Position (kb)
3611
3612
3613 Strain fitness (log2 ratio)
-2
-1
0
1 at 3610.493 kb on - strand, within BWI76_RS17400 at 3611.016 kb on - strand, within BWI76_RS17405 at 3611.016 kb on - strand, within BWI76_RS17405 at 3611.082 kb on + strand at 3611.092 kb on + strand, within BWI76_RS17405 at 3611.427 kb on + strand at 3611.437 kb on + strand at 3611.539 kb on + strand, within BWI76_RS17410 at 3611.573 kb on + strand, within BWI76_RS17410 at 3611.807 kb on + strand, within BWI76_RS17410 at 3611.807 kb on + strand, within BWI76_RS17410 at 3611.807 kb on + strand, within BWI76_RS17410 at 3611.882 kb on - strand, within BWI76_RS17410
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 3,610,493 - BWI76_RS17400 0.63 +0.0 3,611,016 - BWI76_RS17405 0.52 +0.8 3,611,016 - BWI76_RS17405 0.52 -0.6 3,611,082 + +0.1 3,611,092 + BWI76_RS17405 0.85 -2.3 3,611,427 + -0.5 3,611,437 + -0.1 3,611,539 + BWI76_RS17410 0.13 +0.8 3,611,573 + BWI76_RS17410 0.16 +0.2 3,611,807 + BWI76_RS17410 0.38 -1.0 3,611,807 + BWI76_RS17410 0.38 -0.4 3,611,807 + BWI76_RS17410 0.38 -0.9 3,611,882 - BWI76_RS17410 0.45 +0.1
Or see this region's nucleotide sequence