Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17190 and BWI76_RS17195 are separated by 211 nucleotides BWI76_RS17195 and BWI76_RS17200 are separated by 58 nucleotides BWI76_RS17200 and BWI76_RS17205 are separated by 32 nucleotides BWI76_RS17205 and BWI76_RS17210 are separated by 34 nucleotides BWI76_RS17210 and BWI76_RS17215 are separated by 67 nucleotides
BWI76_RS17190: BWI76_RS17190 - TonB system transport protein TonB, at 3,562,317 to 3,563,054
_RS17190
BWI76_RS17195: BWI76_RS17195 - hypothetical protein, at 3,563,266 to 3,563,562
_RS17195
BWI76_RS17200: BWI76_RS17200 - hypothetical protein, at 3,563,621 to 3,563,905
_RS17200
BWI76_RS17205: BWI76_RS17205 - cardiolipin synthase A, at 3,563,938 to 3,565,398
_RS17205
BWI76_RS17210: BWI76_RS17210 - dsDNA-mimic protein, at 3,565,433 to 3,565,762
_RS17210
BWI76_RS17215: BWI76_RS17215 - transporter, at 3,565,830 to 3,566,667
_RS17215
Position (kb)
3563
3564
3565
3566 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3562.960 kb on - strand, within BWI76_RS17190 at 3562.965 kb on - strand, within BWI76_RS17190 at 3563.466 kb on + strand, within BWI76_RS17195 at 3563.639 kb on + strand at 3563.776 kb on - strand, within BWI76_RS17200 at 3563.964 kb on - strand at 3563.964 kb on - strand at 3563.978 kb on - strand at 3564.296 kb on + strand, within BWI76_RS17205 at 3564.342 kb on - strand, within BWI76_RS17205 at 3564.389 kb on - strand, within BWI76_RS17205 at 3564.598 kb on - strand, within BWI76_RS17205 at 3564.913 kb on - strand, within BWI76_RS17205 at 3565.201 kb on - strand, within BWI76_RS17205 at 3565.325 kb on + strand at 3565.400 kb on - strand at 3565.600 kb on - strand, within BWI76_RS17210 at 3565.602 kb on + strand, within BWI76_RS17210 at 3565.607 kb on - strand, within BWI76_RS17210 at 3565.629 kb on + strand, within BWI76_RS17210 at 3565.658 kb on + strand, within BWI76_RS17210 at 3565.702 kb on - strand, within BWI76_RS17210 at 3565.735 kb on - strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.740 kb on + strand at 3565.748 kb on - strand at 3565.748 kb on - strand at 3565.748 kb on - strand at 3565.748 kb on - strand at 3565.748 kb on - strand at 3565.748 kb on - strand at 3565.798 kb on - strand at 3565.841 kb on + strand at 3565.908 kb on + strand at 3565.916 kb on + strand, within BWI76_RS17215 at 3565.916 kb on + strand, within BWI76_RS17215 at 3565.936 kb on + strand, within BWI76_RS17215 at 3566.006 kb on - strand, within BWI76_RS17215 at 3566.034 kb on - strand, within BWI76_RS17215 at 3566.197 kb on + strand, within BWI76_RS17215 at 3566.198 kb on - strand, within BWI76_RS17215 at 3566.207 kb on + strand, within BWI76_RS17215 at 3566.210 kb on - strand, within BWI76_RS17215 at 3566.215 kb on - strand, within BWI76_RS17215 at 3566.220 kb on - strand, within BWI76_RS17215 at 3566.244 kb on + strand, within BWI76_RS17215 at 3566.259 kb on + strand, within BWI76_RS17215 at 3566.321 kb on - strand, within BWI76_RS17215 at 3566.323 kb on + strand, within BWI76_RS17215 at 3566.325 kb on + strand, within BWI76_RS17215 at 3566.326 kb on - strand, within BWI76_RS17215 at 3566.345 kb on + strand, within BWI76_RS17215
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 3,562,960 - BWI76_RS17190 0.87 +1.0 3,562,965 - BWI76_RS17190 0.88 +0.7 3,563,466 + BWI76_RS17195 0.67 +3.5 3,563,639 + -0.0 3,563,776 - BWI76_RS17200 0.54 +1.8 3,563,964 - -1.7 3,563,964 - -3.7 3,563,978 - -0.3 3,564,296 + BWI76_RS17205 0.25 -1.6 3,564,342 - BWI76_RS17205 0.28 -3.2 3,564,389 - BWI76_RS17205 0.31 -3.5 3,564,598 - BWI76_RS17205 0.45 -2.7 3,564,913 - BWI76_RS17205 0.67 -1.5 3,565,201 - BWI76_RS17205 0.86 -0.9 3,565,325 + -1.5 3,565,400 - -1.9 3,565,600 - BWI76_RS17210 0.51 +0.5 3,565,602 + BWI76_RS17210 0.51 +0.0 3,565,607 - BWI76_RS17210 0.53 -1.0 3,565,629 + BWI76_RS17210 0.59 -1.2 3,565,658 + BWI76_RS17210 0.68 -1.0 3,565,702 - BWI76_RS17210 0.82 -0.3 3,565,735 - +1.5 3,565,740 + +0.4 3,565,740 + +1.0 3,565,740 + -0.2 3,565,740 + -1.1 3,565,740 + -0.8 3,565,740 + -0.4 3,565,740 + +0.9 3,565,740 + +0.7 3,565,740 + +0.6 3,565,740 + +0.4 3,565,740 + +1.4 3,565,740 + -1.1 3,565,740 + +0.3 3,565,748 - -0.5 3,565,748 - -0.2 3,565,748 - -0.9 3,565,748 - -0.9 3,565,748 - +0.0 3,565,748 - +1.1 3,565,798 - +1.7 3,565,841 + -0.3 3,565,908 + +1.1 3,565,916 + BWI76_RS17215 0.10 -0.0 3,565,916 + BWI76_RS17215 0.10 -0.8 3,565,936 + BWI76_RS17215 0.13 -0.3 3,566,006 - BWI76_RS17215 0.21 -0.5 3,566,034 - BWI76_RS17215 0.24 +0.9 3,566,197 + BWI76_RS17215 0.44 -1.4 3,566,198 - BWI76_RS17215 0.44 +0.2 3,566,207 + BWI76_RS17215 0.45 -0.3 3,566,210 - BWI76_RS17215 0.45 -1.0 3,566,215 - BWI76_RS17215 0.46 +0.1 3,566,220 - BWI76_RS17215 0.47 +0.5 3,566,244 + BWI76_RS17215 0.49 +0.4 3,566,259 + BWI76_RS17215 0.51 +0.8 3,566,321 - BWI76_RS17215 0.59 -0.9 3,566,323 + BWI76_RS17215 0.59 +0.6 3,566,325 + BWI76_RS17215 0.59 -1.2 3,566,326 - BWI76_RS17215 0.59 -0.8 3,566,345 + BWI76_RS17215 0.61 -2.3
Or see this region's nucleotide sequence