Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17075

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17070 and BWI76_RS17075 are separated by 154 nucleotidesBWI76_RS17075 and BWI76_RS17080 are separated by 117 nucleotidesBWI76_RS17080 and BWI76_RS17085 are separated by 34 nucleotides BWI76_RS17070: BWI76_RS17070 - phosphoenolpyruvate synthase regulatory protein, at 3,542,781 to 3,543,614 _RS17070 BWI76_RS17075: BWI76_RS17075 - 3-deoxy-7-phosphoheptulonate synthase, at 3,543,769 to 3,544,815 _RS17075 BWI76_RS17080: BWI76_RS17080 - hypothetical protein, at 3,544,933 to 3,545,160 _RS17080 BWI76_RS17085: BWI76_RS17085 - hypothetical protein, at 3,545,195 to 3,546,637 _RS17085 Position (kb) 3543 3544 3545Strain fitness (log2 ratio) -3 -2 -1 0 1at 3542.788 kb on + strandat 3542.788 kb on + strandat 3542.788 kb on + strandat 3542.788 kb on + strandat 3542.796 kb on - strandat 3542.796 kb on - strandat 3542.844 kb on + strandat 3542.844 kb on + strandat 3542.844 kb on + strandat 3542.877 kb on + strand, within BWI76_RS17070at 3542.885 kb on + strand, within BWI76_RS17070at 3542.890 kb on + strand, within BWI76_RS17070at 3542.940 kb on + strand, within BWI76_RS17070at 3543.189 kb on - strand, within BWI76_RS17070at 3543.220 kb on + strand, within BWI76_RS17070at 3543.539 kb on - strandat 3543.670 kb on + strandat 3543.910 kb on + strand, within BWI76_RS17075at 3544.161 kb on + strand, within BWI76_RS17075at 3544.279 kb on - strand, within BWI76_RS17075at 3544.310 kb on - strand, within BWI76_RS17075at 3544.444 kb on - strand, within BWI76_RS17075at 3545.073 kb on - strand, within BWI76_RS17080at 3545.109 kb on + strand, within BWI76_RS17080at 3545.112 kb on - strand, within BWI76_RS17080at 3545.117 kb on - strand, within BWI76_RS17080at 3545.121 kb on + strand, within BWI76_RS17080at 3545.122 kb on - strand, within BWI76_RS17080at 3545.140 kb on + strandat 3545.180 kb on - strandat 3545.385 kb on - strand, within BWI76_RS17085at 3545.420 kb on - strand, within BWI76_RS17085at 3545.599 kb on - strand, within BWI76_RS17085

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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3,542,788 + -0.5
3,542,788 + -1.1
3,542,788 + -1.6
3,542,788 + -0.4
3,542,796 - -0.5
3,542,796 - -0.3
3,542,844 + +1.4
3,542,844 + +0.5
3,542,844 + -0.5
3,542,877 + BWI76_RS17070 0.12 -0.5
3,542,885 + BWI76_RS17070 0.12 -0.9
3,542,890 + BWI76_RS17070 0.13 -1.6
3,542,940 + BWI76_RS17070 0.19 -1.3
3,543,189 - BWI76_RS17070 0.49 -0.9
3,543,220 + BWI76_RS17070 0.53 -0.1
3,543,539 - -0.7
3,543,670 + +0.6
3,543,910 + BWI76_RS17075 0.13 -0.4
3,544,161 + BWI76_RS17075 0.37 -1.6
3,544,279 - BWI76_RS17075 0.49 -2.8
3,544,310 - BWI76_RS17075 0.52 -0.6
3,544,444 - BWI76_RS17075 0.64 -1.2
3,545,073 - BWI76_RS17080 0.61 -0.1
3,545,109 + BWI76_RS17080 0.77 +1.1
3,545,112 - BWI76_RS17080 0.79 -1.3
3,545,117 - BWI76_RS17080 0.81 +0.9
3,545,121 + BWI76_RS17080 0.82 -0.4
3,545,122 - BWI76_RS17080 0.83 +0.2
3,545,140 + -1.9
3,545,180 - +0.5
3,545,385 - BWI76_RS17085 0.13 +0.6
3,545,420 - BWI76_RS17085 0.16 +0.3
3,545,599 - BWI76_RS17085 0.28 +0.6

Or see this region's nucleotide sequence