Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14255 and BWI76_RS14260 overlap by 4 nucleotides BWI76_RS14260 and BWI76_RS14265 are separated by 158 nucleotides BWI76_RS14265 and BWI76_RS14270 are separated by 365 nucleotides
BWI76_RS14255: BWI76_RS14255 - terminal oxidase subunit II, at 2,981,774 to 2,982,778
_RS14255
BWI76_RS14260: BWI76_RS14260 - DUF2474 domain-containing protein, at 2,982,775 to 2,982,930
_RS14260
BWI76_RS14265: BWI76_RS14265 - LysR family transcriptional regulator, at 2,983,089 to 2,984,027
_RS14265
BWI76_RS14270: BWI76_RS14270 - transporter, at 2,984,393 to 2,985,205
_RS14270
Position (kb)
2983
2984
2985 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2982.162 kb on + strand, within BWI76_RS14255 at 2982.170 kb on - strand, within BWI76_RS14255 at 2982.227 kb on - strand, within BWI76_RS14255 at 2982.232 kb on - strand, within BWI76_RS14255 at 2982.253 kb on + strand, within BWI76_RS14255 at 2982.261 kb on - strand, within BWI76_RS14255 at 2982.271 kb on + strand, within BWI76_RS14255 at 2982.297 kb on + strand, within BWI76_RS14255 at 2982.366 kb on - strand, within BWI76_RS14255 at 2982.488 kb on + strand, within BWI76_RS14255 at 2982.488 kb on + strand, within BWI76_RS14255 at 2982.854 kb on - strand, within BWI76_RS14260 at 2982.957 kb on + strand at 2982.974 kb on - strand at 2983.006 kb on - strand at 2983.024 kb on + strand at 2983.032 kb on + strand at 2983.039 kb on - strand at 2983.039 kb on - strand at 2983.055 kb on - strand at 2983.171 kb on + strand at 2983.225 kb on + strand, within BWI76_RS14265 at 2983.372 kb on - strand, within BWI76_RS14265 at 2983.406 kb on - strand, within BWI76_RS14265 at 2983.534 kb on + strand, within BWI76_RS14265 at 2983.636 kb on + strand, within BWI76_RS14265 at 2983.757 kb on + strand, within BWI76_RS14265 at 2983.757 kb on + strand, within BWI76_RS14265 at 2983.757 kb on + strand, within BWI76_RS14265 at 2983.765 kb on - strand, within BWI76_RS14265 at 2983.819 kb on + strand, within BWI76_RS14265 at 2983.862 kb on - strand, within BWI76_RS14265 at 2983.862 kb on - strand, within BWI76_RS14265 at 2983.872 kb on - strand, within BWI76_RS14265 at 2983.993 kb on - strand at 2984.010 kb on + strand at 2984.020 kb on + strand at 2984.022 kb on + strand at 2984.029 kb on - strand at 2984.478 kb on - strand, within BWI76_RS14270 at 2984.538 kb on - strand, within BWI76_RS14270 at 2984.642 kb on - strand, within BWI76_RS14270 at 2984.673 kb on + strand, within BWI76_RS14270 at 2984.673 kb on + strand, within BWI76_RS14270 at 2984.775 kb on + strand, within BWI76_RS14270 at 2984.835 kb on - strand at 2984.938 kb on + strand, within BWI76_RS14270 at 2985.009 kb on + strand, within BWI76_RS14270
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 2,982,162 + BWI76_RS14255 0.39 -0.1 2,982,170 - BWI76_RS14255 0.39 +0.2 2,982,227 - BWI76_RS14255 0.45 -0.2 2,982,232 - BWI76_RS14255 0.46 -1.1 2,982,253 + BWI76_RS14255 0.48 +0.3 2,982,261 - BWI76_RS14255 0.48 +0.5 2,982,271 + BWI76_RS14255 0.49 +1.1 2,982,297 + BWI76_RS14255 0.52 +0.2 2,982,366 - BWI76_RS14255 0.59 -0.8 2,982,488 + BWI76_RS14255 0.71 +0.2 2,982,488 + BWI76_RS14255 0.71 +1.2 2,982,854 - BWI76_RS14260 0.51 -1.7 2,982,957 + +0.5 2,982,974 - -0.3 2,983,006 - -2.3 2,983,024 + -0.0 2,983,032 + -0.2 2,983,039 - -1.4 2,983,039 - -0.6 2,983,055 - -0.3 2,983,171 + -0.1 2,983,225 + BWI76_RS14265 0.14 -0.9 2,983,372 - BWI76_RS14265 0.30 -2.8 2,983,406 - BWI76_RS14265 0.34 -0.1 2,983,534 + BWI76_RS14265 0.47 +0.7 2,983,636 + BWI76_RS14265 0.58 -0.4 2,983,757 + BWI76_RS14265 0.71 +1.7 2,983,757 + BWI76_RS14265 0.71 -0.1 2,983,757 + BWI76_RS14265 0.71 -0.6 2,983,765 - BWI76_RS14265 0.72 -1.4 2,983,819 + BWI76_RS14265 0.78 +0.6 2,983,862 - BWI76_RS14265 0.82 +1.7 2,983,862 - BWI76_RS14265 0.82 -0.3 2,983,872 - BWI76_RS14265 0.83 -2.3 2,983,993 - +0.4 2,984,010 + +0.2 2,984,020 + +0.6 2,984,022 + +0.2 2,984,029 - +2.1 2,984,478 - BWI76_RS14270 0.10 -1.4 2,984,538 - BWI76_RS14270 0.18 +0.1 2,984,642 - BWI76_RS14270 0.31 +0.4 2,984,673 + BWI76_RS14270 0.34 -0.6 2,984,673 + BWI76_RS14270 0.34 +2.7 2,984,775 + BWI76_RS14270 0.47 +0.5 2,984,835 - -0.8 2,984,938 + BWI76_RS14270 0.67 -1.3 2,985,009 + BWI76_RS14270 0.76 +0.8
Or see this region's nucleotide sequence