Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10440 and BWI76_RS10445 are separated by 46 nucleotides BWI76_RS10445 and BWI76_RS10450 are separated by 239 nucleotides BWI76_RS10450 and BWI76_RS10455 are separated by 346 nucleotides BWI76_RS10455 and BWI76_RS10460 are separated by 21 nucleotides
BWI76_RS10440: BWI76_RS10440 - protein-disulfide reductase, at 2,206,410 to 2,208,434
_RS10440
BWI76_RS10445: BWI76_RS10445 - hypothetical protein, at 2,208,481 to 2,208,861
_RS10445
BWI76_RS10450: BWI76_RS10450 - tRNA-Tyr, at 2,209,101 to 2,209,175
_RS10450
BWI76_RS10455: BWI76_RS10455 - hypothetical protein, at 2,209,522 to 2,209,728
_RS10455
BWI76_RS10460: BWI76_RS10460 - transcriptional regulator LeuO, at 2,209,750 to 2,210,697
_RS10460
Position (kb)
2208
2209 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2207.489 kb on + strand, within BWI76_RS10440 at 2207.570 kb on + strand, within BWI76_RS10440 at 2207.661 kb on - strand, within BWI76_RS10440 at 2207.890 kb on + strand, within BWI76_RS10440 at 2207.993 kb on + strand, within BWI76_RS10440 at 2208.134 kb on - strand, within BWI76_RS10440 at 2208.164 kb on + strand, within BWI76_RS10440 at 2208.269 kb on + strand at 2208.282 kb on - strand at 2208.357 kb on - strand at 2208.532 kb on + strand, within BWI76_RS10445 at 2208.701 kb on - strand, within BWI76_RS10445 at 2208.741 kb on - strand, within BWI76_RS10445 at 2208.846 kb on + strand at 2208.854 kb on - strand at 2208.953 kb on + strand at 2208.953 kb on + strand at 2208.961 kb on - strand at 2208.961 kb on - strand at 2208.961 kb on - strand at 2208.963 kb on + strand at 2209.081 kb on - strand at 2209.112 kb on - strand, within BWI76_RS10450 at 2209.112 kb on - strand, within BWI76_RS10450 at 2209.145 kb on - strand at 2209.145 kb on - strand, within BWI76_RS10450 at 2209.152 kb on + strand, within BWI76_RS10450 at 2209.190 kb on + strand at 2209.237 kb on + strand at 2209.245 kb on - strand at 2209.326 kb on + strand at 2209.334 kb on - strand at 2209.337 kb on + strand at 2209.337 kb on + strand at 2209.337 kb on + strand at 2209.337 kb on + strand at 2209.345 kb on - strand at 2209.345 kb on - strand at 2209.345 kb on - strand at 2209.345 kb on - strand at 2209.347 kb on + strand at 2209.355 kb on - strand at 2209.395 kb on + strand at 2209.402 kb on - strand at 2209.445 kb on - strand at 2209.484 kb on + strand at 2209.492 kb on - strand at 2209.492 kb on - strand at 2209.492 kb on - strand at 2209.494 kb on + strand at 2209.494 kb on + strand at 2209.554 kb on + strand, within BWI76_RS10455 at 2209.562 kb on - strand, within BWI76_RS10455 at 2209.562 kb on - strand, within BWI76_RS10455 at 2209.573 kb on + strand at 2209.623 kb on + strand, within BWI76_RS10455 at 2209.632 kb on + strand, within BWI76_RS10455 at 2209.637 kb on + strand, within BWI76_RS10455 at 2209.657 kb on + strand, within BWI76_RS10455 at 2209.696 kb on + strand, within BWI76_RS10455 at 2209.696 kb on + strand, within BWI76_RS10455 at 2209.702 kb on + strand, within BWI76_RS10455 at 2209.702 kb on + strand, within BWI76_RS10455 at 2209.702 kb on + strand, within BWI76_RS10455 at 2209.703 kb on - strand, within BWI76_RS10455 at 2209.710 kb on - strand at 2209.710 kb on - strand at 2209.710 kb on - strand at 2209.718 kb on - strand at 2209.741 kb on + strand at 2209.744 kb on + strand at 2209.744 kb on + strand at 2209.774 kb on - strand at 2209.778 kb on + strand at 2209.778 kb on + strand at 2209.809 kb on + strand at 2209.820 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 2,207,489 + BWI76_RS10440 0.53 -1.3 2,207,570 + BWI76_RS10440 0.57 -0.6 2,207,661 - BWI76_RS10440 0.62 -0.0 2,207,890 + BWI76_RS10440 0.73 -0.1 2,207,993 + BWI76_RS10440 0.78 +0.5 2,208,134 - BWI76_RS10440 0.85 +0.7 2,208,164 + BWI76_RS10440 0.87 -0.6 2,208,269 + +0.2 2,208,282 - +0.8 2,208,357 - +0.2 2,208,532 + BWI76_RS10445 0.13 -0.8 2,208,701 - BWI76_RS10445 0.58 -1.1 2,208,741 - BWI76_RS10445 0.68 -0.2 2,208,846 + +0.4 2,208,854 - -0.5 2,208,953 + -1.4 2,208,953 + +0.7 2,208,961 - +0.1 2,208,961 - -0.5 2,208,961 - -1.3 2,208,963 + +0.0 2,209,081 - -0.1 2,209,112 - BWI76_RS10450 0.15 -1.1 2,209,112 - BWI76_RS10450 0.15 +0.1 2,209,145 - +2.6 2,209,145 - BWI76_RS10450 0.59 -0.4 2,209,152 + BWI76_RS10450 0.68 +1.0 2,209,190 + +1.2 2,209,237 + +0.2 2,209,245 - +0.6 2,209,326 + +0.1 2,209,334 - +2.4 2,209,337 + +1.9 2,209,337 + +0.5 2,209,337 + -1.1 2,209,337 + -1.0 2,209,345 - +0.6 2,209,345 - -0.8 2,209,345 - +0.4 2,209,345 - -1.5 2,209,347 + -0.4 2,209,355 - -1.4 2,209,395 + -0.4 2,209,402 - -0.7 2,209,445 - -0.6 2,209,484 + -2.0 2,209,492 - +0.3 2,209,492 - +1.0 2,209,492 - +0.4 2,209,494 + -0.4 2,209,494 + +0.7 2,209,554 + BWI76_RS10455 0.15 -0.0 2,209,562 - BWI76_RS10455 0.19 +0.1 2,209,562 - BWI76_RS10455 0.19 -0.1 2,209,573 + +0.6 2,209,623 + BWI76_RS10455 0.49 -2.4 2,209,632 + BWI76_RS10455 0.53 +0.5 2,209,637 + BWI76_RS10455 0.56 -0.3 2,209,657 + BWI76_RS10455 0.65 -0.9 2,209,696 + BWI76_RS10455 0.84 +0.5 2,209,696 + BWI76_RS10455 0.84 -0.3 2,209,702 + BWI76_RS10455 0.87 +0.4 2,209,702 + BWI76_RS10455 0.87 +0.5 2,209,702 + BWI76_RS10455 0.87 -0.3 2,209,703 - BWI76_RS10455 0.87 -0.6 2,209,710 - -0.5 2,209,710 - -0.3 2,209,710 - +2.0 2,209,718 - +1.0 2,209,741 + -1.1 2,209,744 + -1.3 2,209,744 + -1.1 2,209,774 - -0.8 2,209,778 + -1.8 2,209,778 + +0.5 2,209,809 + -0.9 2,209,820 + +1.5
Or see this region's nucleotide sequence