Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10350 and BWI76_RS10355 are separated by 213 nucleotides BWI76_RS10355 and BWI76_RS10360 overlap by 25 nucleotides BWI76_RS10360 and BWI76_RS10365 are separated by 433 nucleotides
BWI76_RS10350: BWI76_RS10350 - EamA family transporter, at 2,191,884 to 2,192,783
_RS10350
BWI76_RS10355: BWI76_RS10355 - spermidine/putrescine ABC transporter, at 2,192,997 to 2,194,092
_RS10355
BWI76_RS10360: BWI76_RS10360 - TetR family transcriptional regulator, at 2,194,068 to 2,194,646
_RS10360
BWI76_RS10365: BWI76_RS10365 - class IV aminotransferase, at 2,195,080 to 2,196,045
_RS10365
Position (kb)
2192
2193
2194
2195 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2192.049 kb on + strand, within BWI76_RS10350 at 2192.066 kb on + strand, within BWI76_RS10350 at 2192.115 kb on + strand, within BWI76_RS10350 at 2192.118 kb on - strand, within BWI76_RS10350 at 2192.123 kb on - strand, within BWI76_RS10350 at 2192.161 kb on + strand, within BWI76_RS10350 at 2192.204 kb on + strand, within BWI76_RS10350 at 2192.204 kb on + strand, within BWI76_RS10350 at 2192.212 kb on - strand, within BWI76_RS10350 at 2192.212 kb on - strand, within BWI76_RS10350 at 2192.273 kb on + strand, within BWI76_RS10350 at 2192.298 kb on + strand, within BWI76_RS10350 at 2192.298 kb on + strand, within BWI76_RS10350 at 2192.306 kb on - strand, within BWI76_RS10350 at 2192.306 kb on - strand, within BWI76_RS10350 at 2192.306 kb on - strand, within BWI76_RS10350 at 2192.319 kb on + strand, within BWI76_RS10350 at 2192.327 kb on - strand, within BWI76_RS10350 at 2192.359 kb on - strand, within BWI76_RS10350 at 2192.359 kb on - strand, within BWI76_RS10350 at 2192.361 kb on + strand, within BWI76_RS10350 at 2192.369 kb on - strand, within BWI76_RS10350 at 2192.422 kb on - strand, within BWI76_RS10350 at 2192.424 kb on + strand, within BWI76_RS10350 at 2192.481 kb on + strand, within BWI76_RS10350 at 2192.486 kb on - strand, within BWI76_RS10350 at 2192.493 kb on + strand, within BWI76_RS10350 at 2192.581 kb on - strand, within BWI76_RS10350 at 2192.581 kb on - strand, within BWI76_RS10350 at 2192.581 kb on - strand, within BWI76_RS10350 at 2192.808 kb on - strand at 2192.943 kb on + strand at 2192.965 kb on + strand at 2193.055 kb on - strand at 2193.139 kb on - strand, within BWI76_RS10355 at 2193.141 kb on + strand, within BWI76_RS10355 at 2193.149 kb on - strand, within BWI76_RS10355 at 2193.224 kb on + strand, within BWI76_RS10355 at 2193.241 kb on - strand at 2193.241 kb on - strand, within BWI76_RS10355 at 2193.399 kb on + strand, within BWI76_RS10355 at 2193.426 kb on + strand, within BWI76_RS10355 at 2193.548 kb on + strand, within BWI76_RS10355 at 2193.632 kb on + strand, within BWI76_RS10355 at 2193.632 kb on + strand, within BWI76_RS10355 at 2193.818 kb on + strand, within BWI76_RS10355 at 2193.855 kb on - strand, within BWI76_RS10355 at 2193.855 kb on - strand, within BWI76_RS10355 at 2193.978 kb on + strand, within BWI76_RS10355 at 2194.443 kb on + strand, within BWI76_RS10360 at 2194.673 kb on - strand at 2194.742 kb on + strand at 2194.883 kb on + strand at 2194.967 kb on + strand at 2195.026 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 2,192,049 + BWI76_RS10350 0.18 -1.3 2,192,066 + BWI76_RS10350 0.20 +1.0 2,192,115 + BWI76_RS10350 0.26 +0.1 2,192,118 - BWI76_RS10350 0.26 -1.1 2,192,123 - BWI76_RS10350 0.27 -0.9 2,192,161 + BWI76_RS10350 0.31 -0.3 2,192,204 + BWI76_RS10350 0.36 -0.5 2,192,204 + BWI76_RS10350 0.36 +0.1 2,192,212 - BWI76_RS10350 0.36 -0.6 2,192,212 - BWI76_RS10350 0.36 -0.8 2,192,273 + BWI76_RS10350 0.43 +0.5 2,192,298 + BWI76_RS10350 0.46 -1.2 2,192,298 + BWI76_RS10350 0.46 +0.9 2,192,306 - BWI76_RS10350 0.47 +3.0 2,192,306 - BWI76_RS10350 0.47 -2.5 2,192,306 - BWI76_RS10350 0.47 -1.4 2,192,319 + BWI76_RS10350 0.48 -0.3 2,192,327 - BWI76_RS10350 0.49 +1.4 2,192,359 - BWI76_RS10350 0.53 -0.9 2,192,359 - BWI76_RS10350 0.53 +0.0 2,192,361 + BWI76_RS10350 0.53 -0.4 2,192,369 - BWI76_RS10350 0.54 -1.6 2,192,422 - BWI76_RS10350 0.60 +0.2 2,192,424 + BWI76_RS10350 0.60 +0.4 2,192,481 + BWI76_RS10350 0.66 -0.3 2,192,486 - BWI76_RS10350 0.67 -0.8 2,192,493 + BWI76_RS10350 0.68 -1.2 2,192,581 - BWI76_RS10350 0.77 +0.0 2,192,581 - BWI76_RS10350 0.77 +0.2 2,192,581 - BWI76_RS10350 0.77 -0.1 2,192,808 - -0.4 2,192,943 + +0.0 2,192,965 + +1.5 2,193,055 - +4.0 2,193,139 - BWI76_RS10355 0.13 -0.1 2,193,141 + BWI76_RS10355 0.13 +1.1 2,193,149 - BWI76_RS10355 0.14 +0.4 2,193,224 + BWI76_RS10355 0.21 +1.8 2,193,241 - +0.3 2,193,241 - BWI76_RS10355 0.22 -1.7 2,193,399 + BWI76_RS10355 0.37 +1.0 2,193,426 + BWI76_RS10355 0.39 +0.3 2,193,548 + BWI76_RS10355 0.50 +0.5 2,193,632 + BWI76_RS10355 0.58 +0.0 2,193,632 + BWI76_RS10355 0.58 +0.9 2,193,818 + BWI76_RS10355 0.75 +4.3 2,193,855 - BWI76_RS10355 0.78 +1.2 2,193,855 - BWI76_RS10355 0.78 -0.8 2,193,978 + BWI76_RS10355 0.90 +3.1 2,194,443 + BWI76_RS10360 0.65 -1.2 2,194,673 - +0.4 2,194,742 + -0.2 2,194,883 + +1.5 2,194,967 + -0.2 2,195,026 + +0.2
Or see this region's nucleotide sequence