Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08640 and BWI76_RS08645 are separated by 143 nucleotides BWI76_RS08645 and BWI76_RS08650 overlap by 1 nucleotides BWI76_RS08650 and BWI76_RS08655 are separated by 2 nucleotides
BWI76_RS08640: BWI76_RS08640 - multidrug efflux pump-associated protein, AcrZ family, at 1,823,030 to 1,823,179
_RS08640
BWI76_RS08645: BWI76_RS08645 - molybdate ABC transporter substrate-binding protein, at 1,823,323 to 1,824,096
_RS08645
BWI76_RS08650: BWI76_RS08650 - molybdate ABC transporter permease, at 1,824,096 to 1,824,785
_RS08650
BWI76_RS08655: BWI76_RS08655 - molybdenum ABC transporter ATP-binding protein, at 1,824,788 to 1,825,846
_RS08655
Position (kb)
1824
1825 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1823.226 kb on + strand at 1823.234 kb on - strand at 1823.336 kb on + strand at 1823.433 kb on + strand, within BWI76_RS08645 at 1823.441 kb on - strand, within BWI76_RS08645 at 1823.906 kb on + strand, within BWI76_RS08645 at 1824.126 kb on + strand at 1824.170 kb on - strand, within BWI76_RS08650 at 1824.284 kb on - strand, within BWI76_RS08650 at 1824.809 kb on - strand at 1824.893 kb on + strand at 1824.907 kb on + strand, within BWI76_RS08655 at 1825.150 kb on + strand, within BWI76_RS08655 at 1825.464 kb on - strand, within BWI76_RS08655
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 1,823,226 + +0.6 1,823,234 - +1.2 1,823,336 + +3.0 1,823,433 + BWI76_RS08645 0.14 +2.8 1,823,441 - BWI76_RS08645 0.15 +2.8 1,823,906 + BWI76_RS08645 0.75 +3.1 1,824,126 + +2.7 1,824,170 - BWI76_RS08650 0.11 +2.5 1,824,284 - BWI76_RS08650 0.27 +1.5 1,824,809 - +2.7 1,824,893 + +3.6 1,824,907 + BWI76_RS08655 0.11 +2.7 1,825,150 + BWI76_RS08655 0.34 +3.0 1,825,464 - BWI76_RS08655 0.64 +0.3
Or see this region's nucleotide sequence