Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08620

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08615 and BWI76_RS08620 are separated by 2 nucleotidesBWI76_RS08620 and BWI76_RS08625 are separated by 9 nucleotides BWI76_RS08615: BWI76_RS08615 - galactokinase, at 1,817,121 to 1,818,269 _RS08615 BWI76_RS08620: BWI76_RS08620 - galactose-1-phosphate uridylyltransferase, at 1,818,272 to 1,819,318 _RS08620 BWI76_RS08625: BWI76_RS08625 - UDP-glucose 4-epimerase GalE, at 1,819,328 to 1,820,339 _RS08625 Position (kb) 1818 1819 1820Strain fitness (log2 ratio) -3 -2 -1 0 1at 1817.309 kb on + strand, within BWI76_RS08615at 1817.583 kb on - strand, within BWI76_RS08615at 1817.583 kb on - strand, within BWI76_RS08615at 1817.737 kb on + strand, within BWI76_RS08615at 1817.997 kb on + strand, within BWI76_RS08615at 1818.842 kb on + strand, within BWI76_RS08620at 1818.920 kb on + strand, within BWI76_RS08620at 1818.974 kb on - strand, within BWI76_RS08620at 1818.984 kb on - strand, within BWI76_RS08620at 1819.150 kb on - strand, within BWI76_RS08620at 1819.150 kb on - strand, within BWI76_RS08620at 1819.567 kb on - strand, within BWI76_RS08625at 1819.866 kb on - strand, within BWI76_RS08625

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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1,817,309 + BWI76_RS08615 0.16 -0.7
1,817,583 - BWI76_RS08615 0.40 +0.3
1,817,583 - BWI76_RS08615 0.40 -3.0
1,817,737 + BWI76_RS08615 0.54 -0.4
1,817,997 + BWI76_RS08615 0.76 -0.4
1,818,842 + BWI76_RS08620 0.54 -0.8
1,818,920 + BWI76_RS08620 0.62 -1.7
1,818,974 - BWI76_RS08620 0.67 -1.8
1,818,984 - BWI76_RS08620 0.68 -2.9
1,819,150 - BWI76_RS08620 0.84 -1.2
1,819,150 - BWI76_RS08620 0.84 -1.7
1,819,567 - BWI76_RS08625 0.24 +0.0
1,819,866 - BWI76_RS08625 0.53 +0.8

Or see this region's nucleotide sequence