Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06780

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06775 and BWI76_RS06780 are separated by 22 nucleotidesBWI76_RS06780 and BWI76_RS06785 are separated by 142 nucleotidesBWI76_RS06785 and BWI76_RS06790 are separated by 130 nucleotides BWI76_RS06775: BWI76_RS06775 - multidrug efflux RND transporter permease subunit, at 1,418,843 to 1,421,989 _RS06775 BWI76_RS06780: BWI76_RS06780 - MexE family multidrug efflux RND transporter periplasmic adaptor subunit, at 1,422,012 to 1,423,205 _RS06780 BWI76_RS06785: BWI76_RS06785 - DNA-binding transcriptional repressor AcrR, at 1,423,348 to 1,423,998 _RS06785 BWI76_RS06790: BWI76_RS06790 - mechanosensitive channel MscK, at 1,424,129 to 1,427,470 _RS06790 Position (kb) 1422 1423 1424Strain fitness (log2 ratio) -3 -2 -1 0 1at 1421.142 kb on + strand, within BWI76_RS06775at 1421.212 kb on + strand, within BWI76_RS06775at 1421.257 kb on + strand, within BWI76_RS06775at 1421.450 kb on - strand, within BWI76_RS06775at 1421.719 kb on - strandat 1422.024 kb on - strandat 1422.090 kb on - strandat 1422.300 kb on + strand, within BWI76_RS06780at 1422.692 kb on - strand, within BWI76_RS06780at 1422.782 kb on - strand, within BWI76_RS06780at 1422.802 kb on + strand, within BWI76_RS06780at 1422.810 kb on - strand, within BWI76_RS06780at 1422.831 kb on - strand, within BWI76_RS06780at 1422.985 kb on + strand, within BWI76_RS06780at 1423.032 kb on + strand, within BWI76_RS06780at 1423.034 kb on - strand, within BWI76_RS06780at 1423.140 kb on - strandat 1423.142 kb on + strandat 1423.161 kb on + strandat 1423.430 kb on + strand, within BWI76_RS06785at 1423.562 kb on - strand, within BWI76_RS06785at 1423.660 kb on + strand, within BWI76_RS06785at 1423.786 kb on - strand, within BWI76_RS06785at 1423.786 kb on - strand, within BWI76_RS06785at 1423.906 kb on - strand, within BWI76_RS06785at 1423.906 kb on - strand, within BWI76_RS06785at 1423.916 kb on - strand, within BWI76_RS06785at 1423.916 kb on - strand, within BWI76_RS06785at 1423.976 kb on + strandat 1424.132 kb on - strandat 1424.132 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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1,421,142 + BWI76_RS06775 0.73 -1.7
1,421,212 + BWI76_RS06775 0.75 -2.3
1,421,257 + BWI76_RS06775 0.77 -0.8
1,421,450 - BWI76_RS06775 0.83 -0.2
1,421,719 - -1.6
1,422,024 - -0.1
1,422,090 - -1.3
1,422,300 + BWI76_RS06780 0.24 -1.6
1,422,692 - BWI76_RS06780 0.57 -1.1
1,422,782 - BWI76_RS06780 0.64 -2.5
1,422,802 + BWI76_RS06780 0.66 -2.4
1,422,810 - BWI76_RS06780 0.67 -1.0
1,422,831 - BWI76_RS06780 0.69 -3.0
1,422,985 + BWI76_RS06780 0.81 -0.9
1,423,032 + BWI76_RS06780 0.85 -1.2
1,423,034 - BWI76_RS06780 0.86 -0.4
1,423,140 - -2.1
1,423,142 + -1.6
1,423,161 + -0.9
1,423,430 + BWI76_RS06785 0.13 -0.7
1,423,562 - BWI76_RS06785 0.33 +0.1
1,423,660 + BWI76_RS06785 0.48 +0.6
1,423,786 - BWI76_RS06785 0.67 -0.5
1,423,786 - BWI76_RS06785 0.67 -0.2
1,423,906 - BWI76_RS06785 0.86 -0.3
1,423,906 - BWI76_RS06785 0.86 -0.4
1,423,916 - BWI76_RS06785 0.87 +0.8
1,423,916 - BWI76_RS06785 0.87 -0.2
1,423,976 + -0.2
1,424,132 - +0.8
1,424,132 - +0.0

Or see this region's nucleotide sequence