Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06535 and BWI76_RS06540 are separated by 208 nucleotides BWI76_RS06540 and BWI76_RS06545 are separated by 213 nucleotides BWI76_RS06545 and BWI76_RS06550 are separated by 148 nucleotides BWI76_RS06550 and BWI76_RS06555 are separated by 110 nucleotides
BWI76_RS06535: BWI76_RS06535 - endopeptidase La, at 1,372,701 to 1,375,055
_RS06535
BWI76_RS06540: BWI76_RS06540 - DNA-binding protein HU-beta, at 1,375,264 to 1,375,536
_RS06540
BWI76_RS06545: BWI76_RS06545 - peptidylprolyl isomerase, at 1,375,750 to 1,377,624
_RS06545
BWI76_RS06550: BWI76_RS06550 - competence protein ComEA, at 1,377,773 to 1,378,138
_RS06550
BWI76_RS06555: BWI76_RS06555 - thioesterase superfamily protein, at 1,378,249 to 1,378,650
_RS06555
Position (kb)
1375
1376
1377
1378 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1374.890 kb on + strand at 1374.898 kb on - strand at 1375.143 kb on - strand at 1375.165 kb on + strand at 1375.166 kb on - strand at 1375.170 kb on + strand at 1375.173 kb on - strand at 1375.177 kb on + strand at 1375.199 kb on + strand at 1375.222 kb on + strand at 1375.425 kb on - strand, within BWI76_RS06540 at 1375.507 kb on + strand, within BWI76_RS06540 at 1375.636 kb on + strand at 1375.636 kb on + strand at 1375.836 kb on + strand at 1376.020 kb on + strand, within BWI76_RS06545 at 1376.095 kb on - strand, within BWI76_RS06545 at 1376.234 kb on - strand, within BWI76_RS06545 at 1376.429 kb on + strand, within BWI76_RS06545 at 1376.692 kb on - strand, within BWI76_RS06545 at 1376.720 kb on + strand, within BWI76_RS06545 at 1376.829 kb on + strand at 1377.238 kb on - strand, within BWI76_RS06545 at 1377.660 kb on - strand at 1377.806 kb on + strand at 1377.819 kb on - strand, within BWI76_RS06550 at 1377.918 kb on + strand, within BWI76_RS06550 at 1378.025 kb on + strand, within BWI76_RS06550 at 1378.079 kb on - strand, within BWI76_RS06550
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 1,374,890 + -0.8 1,374,898 - -0.6 1,375,143 - +1.7 1,375,165 + +1.0 1,375,166 - +0.6 1,375,170 + +1.6 1,375,173 - +2.2 1,375,177 + +0.8 1,375,199 + +1.3 1,375,222 + -1.9 1,375,425 - BWI76_RS06540 0.59 -0.2 1,375,507 + BWI76_RS06540 0.89 +2.6 1,375,636 + -1.5 1,375,636 + -2.1 1,375,836 + -4.2 1,376,020 + BWI76_RS06545 0.14 -0.8 1,376,095 - BWI76_RS06545 0.18 -0.2 1,376,234 - BWI76_RS06545 0.26 -2.5 1,376,429 + BWI76_RS06545 0.36 -0.9 1,376,692 - BWI76_RS06545 0.50 -2.1 1,376,720 + BWI76_RS06545 0.52 -1.5 1,376,829 + -0.9 1,377,238 - BWI76_RS06545 0.79 -1.8 1,377,660 - -1.8 1,377,806 + -0.1 1,377,819 - BWI76_RS06550 0.13 +0.1 1,377,918 + BWI76_RS06550 0.40 +0.4 1,378,025 + BWI76_RS06550 0.69 +1.0 1,378,079 - BWI76_RS06550 0.84 -0.5
Or see this region's nucleotide sequence