Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05345 and BWI76_RS05350 overlap by 4 nucleotides BWI76_RS05350 and BWI76_RS05355 are separated by 108 nucleotides BWI76_RS05355 and BWI76_RS05360 are separated by 39 nucleotides BWI76_RS05360 and BWI76_RS05365 overlap by 8 nucleotides
BWI76_RS05345: BWI76_RS05345 - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 1,125,929 to 1,126,636
_RS05345
BWI76_RS05350: BWI76_RS05350 - Rcs stress response system protein RcsF, at 1,126,633 to 1,127,040
_RS05350
BWI76_RS05355: BWI76_RS05355 - methionine ABC transporter substrate-binding protein MetQ, at 1,127,149 to 1,127,964
_RS05355
BWI76_RS05360: BWI76_RS05360 - methionine ABC transporter permease, at 1,128,004 to 1,128,657
_RS05360
BWI76_RS05365: BWI76_RS05365 - D-methionine ABC transporter, ATP-binding protein, at 1,128,650 to 1,129,681
_RS05365
Position (kb) 1127
1128 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1126.227 kb on + strand, within BWI76_RS05345 at 1126.434 kb on + strand, within BWI76_RS05345 at 1126.487 kb on + strand, within BWI76_RS05345 at 1126.507 kb on + strand, within BWI76_RS05345 at 1126.515 kb on - strand, within BWI76_RS05345 at 1126.553 kb on - strand, within BWI76_RS05345 at 1126.555 kb on + strand, within BWI76_RS05345 at 1126.659 kb on + strand at 1126.677 kb on + strand, within BWI76_RS05350 at 1126.695 kb on - strand, within BWI76_RS05350 at 1126.751 kb on + strand, within BWI76_RS05350 at 1126.759 kb on - strand, within BWI76_RS05350 at 1126.954 kb on + strand, within BWI76_RS05350 at 1126.962 kb on - strand, within BWI76_RS05350 at 1126.979 kb on + strand, within BWI76_RS05350 at 1126.986 kb on + strand, within BWI76_RS05350 at 1126.986 kb on + strand, within BWI76_RS05350 at 1126.986 kb on + strand, within BWI76_RS05350 at 1126.989 kb on - strand, within BWI76_RS05350 at 1127.008 kb on - strand at 1127.138 kb on - strand at 1127.155 kb on + strand at 1127.163 kb on - strand at 1127.214 kb on + strand at 1127.221 kb on - strand at 1127.301 kb on - strand, within BWI76_RS05355 at 1127.301 kb on - strand, within BWI76_RS05355 at 1127.302 kb on - strand, within BWI76_RS05355 at 1127.542 kb on + strand, within BWI76_RS05355 at 1127.690 kb on + strand, within BWI76_RS05355 at 1127.693 kb on - strand, within BWI76_RS05355 at 1127.698 kb on - strand, within BWI76_RS05355 at 1127.698 kb on - strand, within BWI76_RS05355 at 1127.892 kb on + strand at 1127.945 kb on - strand at 1128.075 kb on + strand, within BWI76_RS05360 at 1128.081 kb on + strand, within BWI76_RS05360 at 1128.089 kb on + strand, within BWI76_RS05360 at 1128.111 kb on + strand, within BWI76_RS05360 at 1128.206 kb on - strand, within BWI76_RS05360 at 1128.442 kb on - strand, within BWI76_RS05360 at 1128.442 kb on - strand, within BWI76_RS05360 at 1128.691 kb on - strand at 1128.753 kb on - strand at 1128.753 kb on - strand at 1128.898 kb on - strand, within BWI76_RS05365
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 1,126,227 + BWI76_RS05345 0.42 +0.8 1,126,434 + BWI76_RS05345 0.71 +2.6 1,126,487 + BWI76_RS05345 0.79 -0.2 1,126,507 + BWI76_RS05345 0.82 -0.4 1,126,515 - BWI76_RS05345 0.83 -1.3 1,126,553 - BWI76_RS05345 0.88 -2.3 1,126,555 + BWI76_RS05345 0.88 +0.3 1,126,659 + -0.6 1,126,677 + BWI76_RS05350 0.11 -2.8 1,126,695 - BWI76_RS05350 0.15 -2.0 1,126,751 + BWI76_RS05350 0.29 -3.0 1,126,759 - BWI76_RS05350 0.31 -2.5 1,126,954 + BWI76_RS05350 0.79 -1.0 1,126,962 - BWI76_RS05350 0.81 -3.5 1,126,979 + BWI76_RS05350 0.85 -1.9 1,126,986 + BWI76_RS05350 0.87 -1.8 1,126,986 + BWI76_RS05350 0.87 -2.1 1,126,986 + BWI76_RS05350 0.87 -1.9 1,126,989 - BWI76_RS05350 0.87 -0.9 1,127,008 - -2.6 1,127,138 - -0.1 1,127,155 + -0.5 1,127,163 - -0.3 1,127,214 + -3.5 1,127,221 - -0.6 1,127,301 - BWI76_RS05355 0.19 -0.5 1,127,301 - BWI76_RS05355 0.19 -1.8 1,127,302 - BWI76_RS05355 0.19 -1.1 1,127,542 + BWI76_RS05355 0.48 -1.6 1,127,690 + BWI76_RS05355 0.66 -0.8 1,127,693 - BWI76_RS05355 0.67 -0.6 1,127,698 - BWI76_RS05355 0.67 -2.1 1,127,698 - BWI76_RS05355 0.67 -2.1 1,127,892 + -1.4 1,127,945 - -1.4 1,128,075 + BWI76_RS05360 0.11 -3.6 1,128,081 + BWI76_RS05360 0.12 -2.5 1,128,089 + BWI76_RS05360 0.13 -2.0 1,128,111 + BWI76_RS05360 0.16 -1.7 1,128,206 - BWI76_RS05360 0.31 +1.2 1,128,442 - BWI76_RS05360 0.67 +0.3 1,128,442 - BWI76_RS05360 0.67 -0.2 1,128,691 - +0.2 1,128,753 - +0.1 1,128,753 - +0.1 1,128,898 - BWI76_RS05365 0.24 +0.8
Or see this region's nucleotide sequence