Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05350

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05340 and BWI76_RS05345 are separated by 110 nucleotidesBWI76_RS05345 and BWI76_RS05350 overlap by 4 nucleotidesBWI76_RS05350 and BWI76_RS05355 are separated by 108 nucleotidesBWI76_RS05355 and BWI76_RS05360 are separated by 39 nucleotides BWI76_RS05340: BWI76_RS05340 - proline--tRNA ligase, at 1,124,100 to 1,125,818 _RS05340 BWI76_RS05345: BWI76_RS05345 - tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO, at 1,125,929 to 1,126,636 _RS05345 BWI76_RS05350: BWI76_RS05350 - Rcs stress response system protein RcsF, at 1,126,633 to 1,127,040 _RS05350 BWI76_RS05355: BWI76_RS05355 - methionine ABC transporter substrate-binding protein MetQ, at 1,127,149 to 1,127,964 _RS05355 BWI76_RS05360: BWI76_RS05360 - methionine ABC transporter permease, at 1,128,004 to 1,128,657 _RS05360 Position (kb) 1126 1127 1128Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1126.008 kb on + strand, within BWI76_RS05345at 1126.016 kb on - strand, within BWI76_RS05345at 1126.016 kb on - strand, within BWI76_RS05345at 1126.016 kb on - strand, within BWI76_RS05345at 1126.098 kb on - strand, within BWI76_RS05345at 1126.227 kb on + strand, within BWI76_RS05345at 1126.434 kb on + strand, within BWI76_RS05345at 1126.487 kb on + strand, within BWI76_RS05345at 1126.507 kb on + strand, within BWI76_RS05345at 1126.515 kb on - strand, within BWI76_RS05345at 1126.553 kb on - strand, within BWI76_RS05345at 1126.555 kb on + strand, within BWI76_RS05345at 1126.659 kb on + strandat 1126.677 kb on + strand, within BWI76_RS05350at 1126.695 kb on - strand, within BWI76_RS05350at 1126.751 kb on + strand, within BWI76_RS05350at 1126.759 kb on - strand, within BWI76_RS05350at 1126.954 kb on + strand, within BWI76_RS05350at 1126.962 kb on - strand, within BWI76_RS05350at 1126.979 kb on + strand, within BWI76_RS05350at 1126.986 kb on + strand, within BWI76_RS05350at 1126.986 kb on + strand, within BWI76_RS05350at 1126.986 kb on + strand, within BWI76_RS05350at 1126.989 kb on - strand, within BWI76_RS05350at 1127.008 kb on - strandat 1127.138 kb on - strandat 1127.155 kb on + strandat 1127.163 kb on - strandat 1127.214 kb on + strandat 1127.221 kb on - strandat 1127.301 kb on - strand, within BWI76_RS05355at 1127.301 kb on - strand, within BWI76_RS05355at 1127.302 kb on - strand, within BWI76_RS05355at 1127.542 kb on + strand, within BWI76_RS05355at 1127.690 kb on + strand, within BWI76_RS05355at 1127.693 kb on - strand, within BWI76_RS05355at 1127.698 kb on - strand, within BWI76_RS05355at 1127.698 kb on - strand, within BWI76_RS05355at 1127.892 kb on + strandat 1127.945 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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1,126,008 + BWI76_RS05345 0.11 -1.2
1,126,016 - BWI76_RS05345 0.12 -0.6
1,126,016 - BWI76_RS05345 0.12 -0.2
1,126,016 - BWI76_RS05345 0.12 -1.6
1,126,098 - BWI76_RS05345 0.24 -0.4
1,126,227 + BWI76_RS05345 0.42 +0.8
1,126,434 + BWI76_RS05345 0.71 +2.6
1,126,487 + BWI76_RS05345 0.79 -0.2
1,126,507 + BWI76_RS05345 0.82 -0.4
1,126,515 - BWI76_RS05345 0.83 -1.3
1,126,553 - BWI76_RS05345 0.88 -2.3
1,126,555 + BWI76_RS05345 0.88 +0.3
1,126,659 + -0.6
1,126,677 + BWI76_RS05350 0.11 -2.8
1,126,695 - BWI76_RS05350 0.15 -2.0
1,126,751 + BWI76_RS05350 0.29 -3.0
1,126,759 - BWI76_RS05350 0.31 -2.5
1,126,954 + BWI76_RS05350 0.79 -1.0
1,126,962 - BWI76_RS05350 0.81 -3.5
1,126,979 + BWI76_RS05350 0.85 -1.9
1,126,986 + BWI76_RS05350 0.87 -1.8
1,126,986 + BWI76_RS05350 0.87 -2.1
1,126,986 + BWI76_RS05350 0.87 -1.9
1,126,989 - BWI76_RS05350 0.87 -0.9
1,127,008 - -2.6
1,127,138 - -0.1
1,127,155 + -0.5
1,127,163 - -0.3
1,127,214 + -3.5
1,127,221 - -0.6
1,127,301 - BWI76_RS05355 0.19 -0.5
1,127,301 - BWI76_RS05355 0.19 -1.8
1,127,302 - BWI76_RS05355 0.19 -1.1
1,127,542 + BWI76_RS05355 0.48 -1.6
1,127,690 + BWI76_RS05355 0.66 -0.8
1,127,693 - BWI76_RS05355 0.67 -0.6
1,127,698 - BWI76_RS05355 0.67 -2.1
1,127,698 - BWI76_RS05355 0.67 -2.1
1,127,892 + -1.4
1,127,945 - -1.4

Or see this region's nucleotide sequence