Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05005 and BWI76_RS05010 are separated by 51 nucleotides BWI76_RS05010 and BWI76_RS05015 are separated by 61 nucleotides BWI76_RS05015 and BWI76_RS05020 are separated by 164 nucleotides
BWI76_RS05005: BWI76_RS05005 - polynucleotide adenylyltransferase, at 1,049,235 to 1,050,632
_RS05005
BWI76_RS05010: BWI76_RS05010 - tRNA glutamyl-Q synthetase, at 1,050,684 to 1,051,565
_RS05010
BWI76_RS05015: BWI76_RS05015 - transcriptional regulator, at 1,051,627 to 1,052,082
_RS05015
BWI76_RS05020: BWI76_RS05020 - sugar fermentation stimulation protein SfsA, at 1,052,247 to 1,052,963
_RS05020
Position (kb)
1050
1051
1052 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1049.808 kb on - strand, within BWI76_RS05005 at 1050.599 kb on - strand at 1050.692 kb on - strand at 1050.692 kb on - strand at 1050.761 kb on + strand at 1050.844 kb on + strand, within BWI76_RS05010 at 1050.844 kb on + strand, within BWI76_RS05010 at 1050.852 kb on - strand, within BWI76_RS05010 at 1051.312 kb on - strand, within BWI76_RS05010 at 1051.541 kb on + strand at 1051.571 kb on + strand at 1051.676 kb on + strand, within BWI76_RS05015 at 1051.867 kb on - strand, within BWI76_RS05015 at 1051.894 kb on - strand, within BWI76_RS05015 at 1051.955 kb on - strand, within BWI76_RS05015 at 1052.065 kb on + strand at 1052.187 kb on - strand at 1052.187 kb on - strand at 1052.187 kb on - strand at 1052.195 kb on - strand at 1052.195 kb on - strand at 1052.197 kb on - strand at 1052.264 kb on + strand at 1052.286 kb on - strand at 1052.291 kb on - strand at 1052.330 kb on + strand, within BWI76_RS05020 at 1052.381 kb on - strand, within BWI76_RS05020 at 1052.382 kb on + strand, within BWI76_RS05020 at 1052.390 kb on - strand, within BWI76_RS05020 at 1052.395 kb on + strand, within BWI76_RS05020 at 1052.395 kb on + strand, within BWI76_RS05020 at 1052.426 kb on - strand, within BWI76_RS05020 at 1052.524 kb on + strand, within BWI76_RS05020 at 1052.531 kb on - strand, within BWI76_RS05020 at 1052.543 kb on + strand, within BWI76_RS05020
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 1,049,808 - BWI76_RS05005 0.41 -1.9 1,050,599 - -3.1 1,050,692 - -1.0 1,050,692 - -2.1 1,050,761 + -0.4 1,050,844 + BWI76_RS05010 0.18 -2.3 1,050,844 + BWI76_RS05010 0.18 -2.5 1,050,852 - BWI76_RS05010 0.19 -0.7 1,051,312 - BWI76_RS05010 0.71 +0.1 1,051,541 + -2.1 1,051,571 + -0.6 1,051,676 + BWI76_RS05015 0.11 +0.0 1,051,867 - BWI76_RS05015 0.53 +0.7 1,051,894 - BWI76_RS05015 0.59 +1.5 1,051,955 - BWI76_RS05015 0.72 -2.2 1,052,065 + -1.8 1,052,187 - +0.4 1,052,187 - -0.8 1,052,187 - +0.7 1,052,195 - +1.2 1,052,195 - +0.3 1,052,197 - +0.4 1,052,264 + +0.4 1,052,286 - -0.3 1,052,291 - -0.2 1,052,330 + BWI76_RS05020 0.12 +0.2 1,052,381 - BWI76_RS05020 0.19 +0.5 1,052,382 + BWI76_RS05020 0.19 -0.8 1,052,390 - BWI76_RS05020 0.20 -0.0 1,052,395 + BWI76_RS05020 0.21 -0.2 1,052,395 + BWI76_RS05020 0.21 -1.1 1,052,426 - BWI76_RS05020 0.25 -0.5 1,052,524 + BWI76_RS05020 0.39 -0.9 1,052,531 - BWI76_RS05020 0.40 +0.0 1,052,543 + BWI76_RS05020 0.41 +0.2
Or see this region's nucleotide sequence