Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04995 and BWI76_RS05000 are separated by 118 nucleotides BWI76_RS05000 and BWI76_RS05005 overlap by 4 nucleotides BWI76_RS05005 and BWI76_RS05010 are separated by 51 nucleotides BWI76_RS05010 and BWI76_RS05015 are separated by 61 nucleotides
BWI76_RS04995: BWI76_RS04995 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,047,849 to 1,048,640
_RS04995
BWI76_RS05000: BWI76_RS05000 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 1,048,759 to 1,049,238
_RS05000
BWI76_RS05005: BWI76_RS05005 - polynucleotide adenylyltransferase, at 1,049,235 to 1,050,632
_RS05005
BWI76_RS05010: BWI76_RS05010 - tRNA glutamyl-Q synthetase, at 1,050,684 to 1,051,565
_RS05010
BWI76_RS05015: BWI76_RS05015 - transcriptional regulator, at 1,051,627 to 1,052,082
_RS05015
Position (kb) 1049
1050
1051 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1048.361 kb on - strand, within BWI76_RS04995 at 1048.377 kb on - strand, within BWI76_RS04995 at 1048.451 kb on + strand, within BWI76_RS04995 at 1048.477 kb on - strand, within BWI76_RS04995 at 1048.482 kb on + strand, within BWI76_RS04995 at 1049.808 kb on - strand, within BWI76_RS05005 at 1050.599 kb on - strand at 1050.692 kb on - strand at 1050.692 kb on - strand at 1050.761 kb on + strand at 1050.844 kb on + strand, within BWI76_RS05010 at 1050.844 kb on + strand, within BWI76_RS05010 at 1050.852 kb on - strand, within BWI76_RS05010 at 1051.312 kb on - strand, within BWI76_RS05010 at 1051.541 kb on + strand at 1051.571 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 1,048,361 - BWI76_RS04995 0.65 +1.1 1,048,377 - BWI76_RS04995 0.67 +0.1 1,048,451 + BWI76_RS04995 0.76 -0.2 1,048,477 - BWI76_RS04995 0.79 +0.6 1,048,482 + BWI76_RS04995 0.80 +1.0 1,049,808 - BWI76_RS05005 0.41 -1.9 1,050,599 - -3.1 1,050,692 - -1.0 1,050,692 - -2.1 1,050,761 + -0.4 1,050,844 + BWI76_RS05010 0.18 -2.3 1,050,844 + BWI76_RS05010 0.18 -2.5 1,050,852 - BWI76_RS05010 0.19 -0.7 1,051,312 - BWI76_RS05010 0.71 +0.1 1,051,541 + -2.1 1,051,571 + -0.6
Or see this region's nucleotide sequence