Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02530 and BWI76_RS02535 are separated by 92 nucleotides BWI76_RS02535 and BWI76_RS02540 are separated by 75 nucleotides BWI76_RS02540 and BWI76_RS02545 are separated by 65 nucleotides
BWI76_RS02530: BWI76_RS02530 - tRNA dimethylallyltransferase, at 518,914 to 519,864
_RS02530
BWI76_RS02535: BWI76_RS02535 - RNA-binding protein Hfq, at 519,957 to 520,265
_RS02535
BWI76_RS02540: BWI76_RS02540 - GTPase HflX, at 520,341 to 521,621
_RS02540
BWI76_RS02545: BWI76_RS02545 - protease modulator HflK, at 521,687 to 522,943
_RS02545
Position (kb) 520
521
522 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 519.530 kb on + strand, within BWI76_RS02530 at 519.776 kb on + strand at 519.853 kb on + strand at 519.882 kb on + strand at 519.922 kb on + strand at 520.239 kb on + strand at 520.333 kb on + strand at 520.365 kb on - strand at 520.427 kb on - strand at 520.528 kb on - strand, within BWI76_RS02540 at 520.794 kb on - strand, within BWI76_RS02540 at 520.799 kb on - strand, within BWI76_RS02540 at 520.825 kb on - strand, within BWI76_RS02540 at 520.849 kb on + strand, within BWI76_RS02540 at 521.003 kb on + strand, within BWI76_RS02540 at 521.011 kb on - strand, within BWI76_RS02540 at 521.252 kb on + strand, within BWI76_RS02540 at 521.311 kb on + strand, within BWI76_RS02540 at 521.357 kb on + strand, within BWI76_RS02540 at 521.441 kb on + strand, within BWI76_RS02540 at 521.516 kb on - strand at 521.541 kb on + strand at 521.550 kb on - strand at 521.684 kb on + strand at 522.172 kb on - strand, within BWI76_RS02545 at 522.186 kb on - strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.375 kb on + strand, within BWI76_RS02545 at 522.383 kb on - strand, within BWI76_RS02545 at 522.576 kb on - strand, within BWI76_RS02545 at 522.592 kb on + strand, within BWI76_RS02545
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 519,530 + BWI76_RS02530 0.65 -1.6 519,776 + -3.2 519,853 + -3.7 519,882 + -2.1 519,922 + -2.4 520,239 + -3.9 520,333 + -1.0 520,365 - -1.1 520,427 - -4.1 520,528 - BWI76_RS02540 0.15 -0.2 520,794 - BWI76_RS02540 0.35 -2.8 520,799 - BWI76_RS02540 0.36 -2.4 520,825 - BWI76_RS02540 0.38 -5.3 520,849 + BWI76_RS02540 0.40 +1.2 521,003 + BWI76_RS02540 0.52 -1.7 521,011 - BWI76_RS02540 0.52 -1.9 521,252 + BWI76_RS02540 0.71 -1.4 521,311 + BWI76_RS02540 0.76 -2.2 521,357 + BWI76_RS02540 0.79 -3.5 521,441 + BWI76_RS02540 0.86 -2.7 521,516 - -3.5 521,541 + -1.3 521,550 - -1.7 521,684 + -4.2 522,172 - BWI76_RS02545 0.39 -3.0 522,186 - BWI76_RS02545 0.40 -2.4 522,375 + BWI76_RS02545 0.55 -2.9 522,375 + BWI76_RS02545 0.55 -2.2 522,375 + BWI76_RS02545 0.55 -2.2 522,383 - BWI76_RS02545 0.55 -2.9 522,576 - BWI76_RS02545 0.71 -2.5 522,592 + BWI76_RS02545 0.72 -2.3
Or see this region's nucleotide sequence