Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02485 and BWI76_RS02490 are separated by 286 nucleotides BWI76_RS02490 and BWI76_RS02495 are separated by 23 nucleotides BWI76_RS02495 and BWI76_RS02500 are separated by 24 nucleotides BWI76_RS02500 and BWI76_RS02505 are separated by 270 nucleotides BWI76_RS02505 and BWI76_RS02510 overlap by 2 nucleotides
BWI76_RS02485: BWI76_RS02485 - polar amino acid ABC transporter (PAAT) family, periplasmic amino acid-binding protein, at 510,980 to 511,711
_RS02485
BWI76_RS02490: BWI76_RS02490 - tRNA-Gly, at 511,998 to 512,073
_RS02490
BWI76_RS02495: BWI76_RS02495 - tRNA-Gly, at 512,097 to 512,172
_RS02495
BWI76_RS02500: BWI76_RS02500 - tRNA-Gly, at 512,197 to 512,272
_RS02500
BWI76_RS02505: BWI76_RS02505 - tRNA epoxyqueuosine(34) reductase QueG, at 512,543 to 513,682
_RS02505
BWI76_RS02510: BWI76_RS02510 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 513,681 to 515,207
_RS02510
Position (kb) 512
513
514 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 511.550 kb on - strand, within BWI76_RS02485 at 511.559 kb on + strand, within BWI76_RS02485 at 512.582 kb on - strand at 512.584 kb on + strand at 512.689 kb on - strand, within BWI76_RS02505 at 512.733 kb on + strand, within BWI76_RS02505 at 512.733 kb on + strand, within BWI76_RS02505 at 512.733 kb on + strand, within BWI76_RS02505 at 512.868 kb on + strand, within BWI76_RS02505 at 513.045 kb on + strand, within BWI76_RS02505 at 513.201 kb on - strand, within BWI76_RS02505 at 513.203 kb on + strand, within BWI76_RS02505 at 513.354 kb on - strand, within BWI76_RS02505 at 513.354 kb on - strand, within BWI76_RS02505 at 513.389 kb on - strand, within BWI76_RS02505 at 513.481 kb on + strand, within BWI76_RS02505 at 513.567 kb on - strand, within BWI76_RS02505 at 513.610 kb on + strand at 513.617 kb on + strand at 513.622 kb on + strand at 513.625 kb on - strand at 513.712 kb on + strand at 513.752 kb on - strand at 513.778 kb on + strand at 513.778 kb on + strand at 513.778 kb on + strand at 513.778 kb on + strand at 513.786 kb on - strand at 513.913 kb on - strand, within BWI76_RS02510 at 513.935 kb on - strand, within BWI76_RS02510 at 514.099 kb on - strand at 514.101 kb on + strand, within BWI76_RS02510 at 514.101 kb on + strand, within BWI76_RS02510 at 514.104 kb on - strand, within BWI76_RS02510 at 514.109 kb on - strand, within BWI76_RS02510 at 514.156 kb on - strand, within BWI76_RS02510 at 514.194 kb on + strand, within BWI76_RS02510 at 514.203 kb on + strand, within BWI76_RS02510 at 514.250 kb on - strand, within BWI76_RS02510 at 514.310 kb on - strand, within BWI76_RS02510 at 514.327 kb on + strand, within BWI76_RS02510 at 514.639 kb on + strand, within BWI76_RS02510 at 514.666 kb on + strand, within BWI76_RS02510
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 511,550 - BWI76_RS02485 0.78 -0.2 511,559 + BWI76_RS02485 0.79 +0.0 512,582 - -1.7 512,584 + -1.6 512,689 - BWI76_RS02505 0.13 -1.1 512,733 + BWI76_RS02505 0.17 -3.1 512,733 + BWI76_RS02505 0.17 -1.9 512,733 + BWI76_RS02505 0.17 -1.2 512,868 + BWI76_RS02505 0.29 -2.5 513,045 + BWI76_RS02505 0.44 -1.0 513,201 - BWI76_RS02505 0.58 -0.9 513,203 + BWI76_RS02505 0.58 -1.2 513,354 - BWI76_RS02505 0.71 -3.7 513,354 - BWI76_RS02505 0.71 -1.3 513,389 - BWI76_RS02505 0.74 -1.0 513,481 + BWI76_RS02505 0.82 -2.3 513,567 - BWI76_RS02505 0.90 -2.2 513,610 + +0.4 513,617 + -0.2 513,622 + -0.7 513,625 - -2.0 513,712 + -1.2 513,752 - -0.0 513,778 + +0.8 513,778 + +0.4 513,778 + +3.9 513,778 + +1.9 513,786 - -1.5 513,913 - BWI76_RS02510 0.15 -0.3 513,935 - BWI76_RS02510 0.17 -0.2 514,099 - -0.0 514,101 + BWI76_RS02510 0.28 +1.8 514,101 + BWI76_RS02510 0.28 +0.3 514,104 - BWI76_RS02510 0.28 -0.6 514,109 - BWI76_RS02510 0.28 -0.1 514,156 - BWI76_RS02510 0.31 -0.4 514,194 + BWI76_RS02510 0.34 -0.7 514,203 + BWI76_RS02510 0.34 +1.6 514,250 - BWI76_RS02510 0.37 -1.3 514,310 - BWI76_RS02510 0.41 +0.9 514,327 + BWI76_RS02510 0.42 +0.9 514,639 + BWI76_RS02510 0.63 +1.8 514,666 + BWI76_RS02510 0.65 -1.7
Or see this region's nucleotide sequence