Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02470

Experiment: Sisomicin 0.008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02465 and BWI76_RS02470 are separated by 24 nucleotidesBWI76_RS02470 and BWI76_RS02475 are separated by 93 nucleotides BWI76_RS02465: BWI76_RS02465 - miniconductance mechanosensitive channel MscM, at 504,830 to 508,156 _RS02465 BWI76_RS02470: BWI76_RS02470 - phosphatidylserine decarboxylase, at 508,181 to 509,143 _RS02470 BWI76_RS02475: BWI76_RS02475 - ribosome small subunit-dependent GTPase, at 509,237 to 510,298 _RS02475 Position (kb) 508 509 510Strain fitness (log2 ratio) -2 -1 0 1 2 3at 507.301 kb on + strand, within BWI76_RS02465at 507.374 kb on - strand, within BWI76_RS02465at 507.506 kb on - strand, within BWI76_RS02465at 507.567 kb on + strand, within BWI76_RS02465at 507.631 kb on + strand, within BWI76_RS02465at 507.653 kb on - strand, within BWI76_RS02465at 507.806 kb on - strand, within BWI76_RS02465at 507.852 kb on - strandat 507.856 kb on + strandat 507.864 kb on - strandat 507.993 kb on - strandat 508.023 kb on + strandat 508.031 kb on - strandat 509.236 kb on - strandat 509.236 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml
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507,301 + BWI76_RS02465 0.74 -1.8
507,374 - BWI76_RS02465 0.76 -1.4
507,506 - BWI76_RS02465 0.80 +2.9
507,567 + BWI76_RS02465 0.82 -0.8
507,631 + BWI76_RS02465 0.84 -0.7
507,653 - BWI76_RS02465 0.85 +0.6
507,806 - BWI76_RS02465 0.89 -0.9
507,852 - -0.5
507,856 + -1.9
507,864 - -1.7
507,993 - +0.2
508,023 + -0.7
508,031 - -2.0
509,236 - +1.5
509,236 - +1.3

Or see this region's nucleotide sequence