Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02345 and BWI76_RS02350 are separated by 234 nucleotides BWI76_RS02350 and BWI76_RS02355 are separated by 43 nucleotides BWI76_RS02355 and BWI76_RS02360 are separated by 134 nucleotides BWI76_RS02360 and BWI76_RS02365 are separated by 86 nucleotides
BWI76_RS02345: BWI76_RS02345 - L-methionine/branched-chain amino acid transporter, at 481,681 to 482,940
_RS02345
BWI76_RS02350: BWI76_RS02350 - molecular chaperone GroES, at 483,175 to 483,468
_RS02350
BWI76_RS02355: BWI76_RS02355 - molecular chaperone GroEL, at 483,512 to 485,158
_RS02355
BWI76_RS02360: BWI76_RS02360 - hypothetical protein, at 485,293 to 485,646
_RS02360
BWI76_RS02365: BWI76_RS02365 - hypothetical protein, at 485,733 to 486,596
_RS02365
Position (kb) 483
484
485
486 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 482.512 kb on + strand, within BWI76_RS02345 at 482.610 kb on - strand, within BWI76_RS02345 at 482.626 kb on + strand, within BWI76_RS02345 at 482.634 kb on - strand, within BWI76_RS02345 at 482.634 kb on - strand, within BWI76_RS02345 at 482.833 kb on + strand at 482.955 kb on - strand at 483.479 kb on + strand at 485.083 kb on + strand at 485.174 kb on - strand at 485.259 kb on - strand at 485.329 kb on + strand, within BWI76_RS02360 at 485.329 kb on + strand, within BWI76_RS02360 at 485.342 kb on - strand, within BWI76_RS02360 at 485.342 kb on - strand, within BWI76_RS02360 at 485.414 kb on - strand, within BWI76_RS02360 at 485.435 kb on - strand, within BWI76_RS02360 at 485.472 kb on + strand, within BWI76_RS02360 at 485.480 kb on - strand, within BWI76_RS02360 at 485.517 kb on + strand, within BWI76_RS02360 at 485.550 kb on + strand, within BWI76_RS02360 at 485.591 kb on + strand, within BWI76_RS02360 at 485.591 kb on + strand, within BWI76_RS02360 at 485.599 kb on - strand, within BWI76_RS02360 at 485.599 kb on - strand, within BWI76_RS02360 at 485.625 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.626 kb on + strand at 485.634 kb on - strand at 485.634 kb on - strand at 485.634 kb on - strand at 485.634 kb on - strand at 485.645 kb on + strand at 485.656 kb on + strand at 485.656 kb on + strand at 485.657 kb on + strand at 485.662 kb on + strand at 485.662 kb on + strand at 485.664 kb on - strand at 485.665 kb on - strand at 485.669 kb on + strand at 485.670 kb on - strand at 485.672 kb on + strand at 485.680 kb on + strand at 485.683 kb on - strand at 485.712 kb on - strand at 485.738 kb on - strand at 485.738 kb on - strand at 485.771 kb on + strand at 485.788 kb on + strand at 485.796 kb on - strand at 485.796 kb on - strand at 485.796 kb on - strand at 485.796 kb on - strand at 485.796 kb on - strand at 485.861 kb on - strand, within BWI76_RS02365 at 485.954 kb on + strand, within BWI76_RS02365 at 485.989 kb on + strand at 486.097 kb on + strand, within BWI76_RS02365 at 486.105 kb on - strand, within BWI76_RS02365 at 486.105 kb on - strand, within BWI76_RS02365 at 486.112 kb on - strand, within BWI76_RS02365
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 482,512 + BWI76_RS02345 0.66 -0.7 482,610 - BWI76_RS02345 0.74 +2.3 482,626 + BWI76_RS02345 0.75 +0.1 482,634 - BWI76_RS02345 0.76 +0.6 482,634 - BWI76_RS02345 0.76 -1.3 482,833 + +1.1 482,955 - -0.7 483,479 + +2.9 485,083 + -2.8 485,174 - +1.9 485,259 - +1.6 485,329 + BWI76_RS02360 0.10 +0.8 485,329 + BWI76_RS02360 0.10 -0.1 485,342 - BWI76_RS02360 0.14 -0.4 485,342 - BWI76_RS02360 0.14 +1.3 485,414 - BWI76_RS02360 0.34 +0.1 485,435 - BWI76_RS02360 0.40 -0.1 485,472 + BWI76_RS02360 0.51 +0.6 485,480 - BWI76_RS02360 0.53 +0.2 485,517 + BWI76_RS02360 0.63 +0.4 485,550 + BWI76_RS02360 0.73 +0.4 485,591 + BWI76_RS02360 0.84 -0.2 485,591 + BWI76_RS02360 0.84 +0.5 485,599 - BWI76_RS02360 0.86 -1.7 485,599 - BWI76_RS02360 0.86 +1.1 485,625 + +0.2 485,626 + +1.0 485,626 + +0.9 485,626 + +0.9 485,626 + +0.3 485,626 + +0.3 485,626 + +1.0 485,626 + -0.9 485,626 + +0.6 485,626 + +0.1 485,634 - -0.3 485,634 - +0.3 485,634 - -1.0 485,634 - -0.1 485,645 + +0.0 485,656 + +1.3 485,656 + +0.2 485,657 + +1.3 485,662 + -0.4 485,662 + +0.8 485,664 - -0.6 485,665 - -0.6 485,669 + +0.1 485,670 - -0.5 485,672 + -0.5 485,680 + +0.5 485,683 - -0.1 485,712 - -0.5 485,738 - -1.7 485,738 - +0.0 485,771 + +0.0 485,788 + -0.9 485,796 - -0.1 485,796 - +1.6 485,796 - -0.9 485,796 - +0.3 485,796 - +0.6 485,861 - BWI76_RS02365 0.15 +1.6 485,954 + BWI76_RS02365 0.26 -0.1 485,989 + +2.6 486,097 + BWI76_RS02365 0.42 -0.5 486,105 - BWI76_RS02365 0.43 -0.6 486,105 - BWI76_RS02365 0.43 +0.6 486,112 - BWI76_RS02365 0.44 -1.9
Or see this region's nucleotide sequence