Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01975 and BWI76_RS01980 are separated by 319 nucleotides BWI76_RS01980 and BWI76_RS01985 are separated by 145 nucleotides BWI76_RS01985 and BWI76_RS01990 are separated by 544 nucleotides
BWI76_RS01975: BWI76_RS01975 - outer membrane protein assembly factor BamA, at 405,031 to 407,454
_RS01975
BWI76_RS01980: BWI76_RS01980 - anti-adapter protein IraM, at 407,774 to 408,163
_RS01980
BWI76_RS01985: BWI76_RS01985 - membrane protein, at 408,309 to 408,590
_RS01985
BWI76_RS01990: BWI76_RS01990 - cyclic diguanylate phosphodiesterase, at 409,135 to 410,739
_RS01990
Position (kb) 408
409 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 407.317 kb on + strand at 407.317 kb on + strand at 407.317 kb on + strand at 407.325 kb on - strand at 407.325 kb on - strand at 407.325 kb on - strand at 407.336 kb on + strand at 407.338 kb on + strand at 407.341 kb on - strand at 407.346 kb on + strand at 407.346 kb on - strand at 407.354 kb on - strand at 407.356 kb on + strand at 407.356 kb on + strand at 407.357 kb on + strand at 407.368 kb on + strand at 407.370 kb on + strand at 407.376 kb on + strand at 407.376 kb on + strand at 407.376 kb on + strand at 407.384 kb on - strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.409 kb on + strand at 407.414 kb on + strand at 407.414 kb on + strand at 407.414 kb on + strand at 407.414 kb on + strand at 407.414 kb on + strand at 407.416 kb on + strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.417 kb on - strand at 407.422 kb on - strand at 407.422 kb on - strand at 407.422 kb on - strand at 407.422 kb on - strand at 407.422 kb on - strand at 407.424 kb on + strand at 407.424 kb on + strand at 407.468 kb on - strand at 407.471 kb on + strand at 407.476 kb on + strand at 407.476 kb on + strand at 407.479 kb on - strand at 407.482 kb on - strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.507 kb on + strand at 407.515 kb on - strand at 407.515 kb on - strand at 407.515 kb on - strand at 407.520 kb on + strand at 407.520 kb on + strand at 407.520 kb on + strand at 407.520 kb on + strand at 407.527 kb on + strand at 407.527 kb on + strand at 407.527 kb on + strand at 407.528 kb on - strand at 407.528 kb on - strand at 407.528 kb on - strand at 407.530 kb on - strand at 407.538 kb on - strand at 407.575 kb on + strand at 407.583 kb on - strand at 407.583 kb on - strand at 407.594 kb on - strand at 407.639 kb on + strand at 407.639 kb on + strand at 407.639 kb on + strand at 407.647 kb on - strand at 407.651 kb on - strand at 407.653 kb on - strand at 407.655 kb on + strand at 407.655 kb on + strand at 407.655 kb on + strand at 407.655 kb on + strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.663 kb on - strand at 407.710 kb on - strand at 407.714 kb on + strand at 407.715 kb on - strand at 407.719 kb on + strand at 407.722 kb on - strand at 407.727 kb on - strand at 407.729 kb on + strand at 407.731 kb on + strand at 407.737 kb on - strand at 407.755 kb on - strand at 407.760 kb on - strand at 407.784 kb on - strand at 407.786 kb on + strand at 407.786 kb on + strand at 407.794 kb on - strand at 407.794 kb on - strand at 407.794 kb on - strand at 407.794 kb on - strand at 407.794 kb on - strand at 407.795 kb on + strand at 407.795 kb on + strand at 407.795 kb on + strand at 407.810 kb on + strand at 407.840 kb on + strand, within BWI76_RS01980 at 407.849 kb on - strand, within BWI76_RS01980 at 407.909 kb on + strand, within BWI76_RS01980 at 407.919 kb on + strand, within BWI76_RS01980 at 407.919 kb on + strand, within BWI76_RS01980 at 407.927 kb on + strand, within BWI76_RS01980 at 407.935 kb on - strand, within BWI76_RS01980 at 407.952 kb on - strand, within BWI76_RS01980 at 407.960 kb on + strand, within BWI76_RS01980 at 407.960 kb on + strand, within BWI76_RS01980 at 407.960 kb on - strand, within BWI76_RS01980 at 407.982 kb on + strand, within BWI76_RS01980 at 407.990 kb on - strand, within BWI76_RS01980 at 407.994 kb on + strand, within BWI76_RS01980 at 407.998 kb on - strand, within BWI76_RS01980 at 408.000 kb on + strand, within BWI76_RS01980 at 408.000 kb on - strand, within BWI76_RS01980 at 408.013 kb on + strand, within BWI76_RS01980 at 408.016 kb on + strand, within BWI76_RS01980 at 408.045 kb on + strand, within BWI76_RS01980 at 408.052 kb on + strand, within BWI76_RS01980 at 408.052 kb on + strand, within BWI76_RS01980 at 408.052 kb on + strand, within BWI76_RS01980 at 408.054 kb on + strand, within BWI76_RS01980 at 408.060 kb on - strand, within BWI76_RS01980 at 408.060 kb on - strand, within BWI76_RS01980 at 408.073 kb on - strand, within BWI76_RS01980 at 408.113 kb on + strand, within BWI76_RS01980 at 408.113 kb on + strand, within BWI76_RS01980 at 408.113 kb on + strand, within BWI76_RS01980 at 408.121 kb on - strand, within BWI76_RS01980 at 408.121 kb on - strand, within BWI76_RS01980 at 408.126 kb on + strand at 408.134 kb on - strand at 408.166 kb on - strand at 408.200 kb on - strand at 408.208 kb on + strand at 408.208 kb on + strand at 408.247 kb on + strand at 408.255 kb on - strand at 408.255 kb on - strand at 408.265 kb on - strand at 408.283 kb on - strand at 408.394 kb on + strand, within BWI76_RS01985 at 408.449 kb on + strand, within BWI76_RS01985 at 408.449 kb on + strand, within BWI76_RS01985 at 408.457 kb on - strand, within BWI76_RS01985 at 408.457 kb on - strand, within BWI76_RS01985 at 408.470 kb on + strand, within BWI76_RS01985 at 408.498 kb on - strand at 408.528 kb on + strand, within BWI76_RS01985 at 408.528 kb on + strand, within BWI76_RS01985 at 408.528 kb on + strand, within BWI76_RS01985 at 408.541 kb on - strand, within BWI76_RS01985 at 408.546 kb on - strand, within BWI76_RS01985 at 408.553 kb on - strand, within BWI76_RS01985 at 408.563 kb on - strand at 408.592 kb on + strand at 408.654 kb on + strand at 408.772 kb on - strand at 408.880 kb on + strand at 408.939 kb on + strand at 409.009 kb on - strand at 409.030 kb on + strand at 409.035 kb on + strand at 409.038 kb on - strand at 409.043 kb on - strand at 409.049 kb on + strand at 409.049 kb on + strand at 409.062 kb on - strand at 409.062 kb on - strand at 409.088 kb on + strand at 409.088 kb on + strand at 409.088 kb on + strand at 409.088 kb on + strand at 409.096 kb on - strand at 409.102 kb on + strand at 409.102 kb on + strand at 409.102 kb on + strand at 409.102 kb on + strand at 409.110 kb on - strand at 409.110 kb on - strand at 409.110 kb on - strand at 409.110 kb on - strand at 409.110 kb on - strand at 409.137 kb on + strand at 409.180 kb on + strand at 409.180 kb on + strand at 409.184 kb on + strand at 409.194 kb on - strand at 409.204 kb on + strand at 409.217 kb on + strand at 409.222 kb on + strand at 409.235 kb on - strand at 409.329 kb on - strand, within BWI76_RS01990 at 409.374 kb on + strand, within BWI76_RS01990 at 409.381 kb on - strand, within BWI76_RS01990 at 409.404 kb on + strand, within BWI76_RS01990 at 409.412 kb on - strand, within BWI76_RS01990 at 409.412 kb on - strand, within BWI76_RS01990 at 409.417 kb on + strand, within BWI76_RS01990 at 409.571 kb on + strand, within BWI76_RS01990 at 409.571 kb on + strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 407,317 + -0.4 407,317 + -0.8 407,317 + +0.5 407,325 - -0.0 407,325 - -1.2 407,325 - +0.7 407,336 + +2.6 407,338 + -2.3 407,341 - -0.4 407,346 + +0.0 407,346 - +0.6 407,354 - -0.3 407,356 + +0.8 407,356 + -1.2 407,357 + +1.0 407,368 + -0.9 407,370 + -0.7 407,376 + -0.3 407,376 + +0.1 407,376 + -0.7 407,384 - -1.7 407,409 + -0.7 407,409 + +0.3 407,409 + -0.5 407,409 + -0.1 407,409 + -0.7 407,409 + -0.0 407,409 + +0.4 407,409 + -0.5 407,409 + -0.2 407,409 + +0.3 407,409 + +0.1 407,409 + +0.3 407,409 + -1.8 407,409 + +0.6 407,414 + -1.9 407,414 + -0.2 407,414 + -3.2 407,414 + -1.7 407,414 + +1.7 407,416 + +0.6 407,417 - -1.2 407,417 - -1.1 407,417 - -1.2 407,417 - -0.7 407,417 - +0.2 407,417 - +1.5 407,417 - +1.3 407,417 - -1.7 407,417 - +0.4 407,417 - -1.4 407,417 - -0.4 407,417 - -1.5 407,422 - -0.2 407,422 - +0.6 407,422 - +0.2 407,422 - +2.6 407,422 - +0.3 407,424 + +5.9 407,424 + -1.1 407,468 - -0.4 407,471 + -0.3 407,476 + +0.6 407,476 + -1.2 407,479 - -0.7 407,482 - +0.6 407,507 + -2.0 407,507 + -0.8 407,507 + -0.4 407,507 + +0.1 407,507 + -1.2 407,507 + -0.4 407,507 + -1.0 407,507 + -0.6 407,507 + +0.2 407,507 + -1.1 407,507 + -1.0 407,507 + -0.6 407,507 + -0.9 407,507 + +0.6 407,515 - +0.1 407,515 - +0.7 407,515 - -2.0 407,520 + +1.1 407,520 + -0.1 407,520 + -0.4 407,520 + +1.9 407,527 + -0.7 407,527 + +0.8 407,527 + -1.7 407,528 - +0.2 407,528 - -1.1 407,528 - +1.0 407,530 - +1.6 407,538 - -1.0 407,575 + -0.1 407,583 - -1.4 407,583 - -1.0 407,594 - +0.2 407,639 + -1.8 407,639 + +0.8 407,639 + +0.6 407,647 - -1.3 407,651 - -0.9 407,653 - -0.0 407,655 + -0.2 407,655 + +0.0 407,655 + -0.8 407,655 + -1.2 407,663 - -1.7 407,663 - -0.2 407,663 - +0.1 407,663 - -0.1 407,663 - -1.3 407,663 - -2.2 407,663 - -1.6 407,663 - -1.9 407,710 - -0.0 407,714 + -3.0 407,715 - +0.1 407,719 + -1.0 407,722 - +0.5 407,727 - -0.5 407,729 + +0.1 407,731 + -0.7 407,737 - +0.4 407,755 - +0.5 407,760 - -0.2 407,784 - -0.1 407,786 + +0.1 407,786 + +0.3 407,794 - -1.5 407,794 - -1.0 407,794 - -1.5 407,794 - -0.4 407,794 - -0.7 407,795 + +0.4 407,795 + -0.3 407,795 + +0.3 407,810 + +1.6 407,840 + BWI76_RS01980 0.17 +2.0 407,849 - BWI76_RS01980 0.19 -1.8 407,909 + BWI76_RS01980 0.35 -0.7 407,919 + BWI76_RS01980 0.37 -0.1 407,919 + BWI76_RS01980 0.37 -0.4 407,927 + BWI76_RS01980 0.39 -1.0 407,935 - BWI76_RS01980 0.41 -0.1 407,952 - BWI76_RS01980 0.46 -0.4 407,960 + BWI76_RS01980 0.48 -1.0 407,960 + BWI76_RS01980 0.48 -0.6 407,960 - BWI76_RS01980 0.48 -0.3 407,982 + BWI76_RS01980 0.53 -0.2 407,990 - BWI76_RS01980 0.55 -0.4 407,994 + BWI76_RS01980 0.56 -0.6 407,998 - BWI76_RS01980 0.57 -1.1 408,000 + BWI76_RS01980 0.58 -1.7 408,000 - BWI76_RS01980 0.58 -1.4 408,013 + BWI76_RS01980 0.61 +0.0 408,016 + BWI76_RS01980 0.62 +0.4 408,045 + BWI76_RS01980 0.69 -0.2 408,052 + BWI76_RS01980 0.71 -1.5 408,052 + BWI76_RS01980 0.71 +0.4 408,052 + BWI76_RS01980 0.71 +0.0 408,054 + BWI76_RS01980 0.72 +1.4 408,060 - BWI76_RS01980 0.73 +1.0 408,060 - BWI76_RS01980 0.73 +0.4 408,073 - BWI76_RS01980 0.77 -0.7 408,113 + BWI76_RS01980 0.87 -0.3 408,113 + BWI76_RS01980 0.87 -0.5 408,113 + BWI76_RS01980 0.87 +0.2 408,121 - BWI76_RS01980 0.89 -0.9 408,121 - BWI76_RS01980 0.89 +0.4 408,126 + -0.0 408,134 - -2.2 408,166 - -1.1 408,200 - -0.8 408,208 + -0.2 408,208 + -1.1 408,247 + -0.1 408,255 - -1.7 408,255 - -0.6 408,265 - +0.1 408,283 - -0.5 408,394 + BWI76_RS01985 0.30 +1.9 408,449 + BWI76_RS01985 0.50 +0.2 408,449 + BWI76_RS01985 0.50 +0.0 408,457 - BWI76_RS01985 0.52 +0.2 408,457 - BWI76_RS01985 0.52 +0.2 408,470 + BWI76_RS01985 0.57 +1.4 408,498 - +0.0 408,528 + BWI76_RS01985 0.78 +1.8 408,528 + BWI76_RS01985 0.78 +0.9 408,528 + BWI76_RS01985 0.78 -0.9 408,541 - BWI76_RS01985 0.82 +2.2 408,546 - BWI76_RS01985 0.84 +0.3 408,553 - BWI76_RS01985 0.87 +0.6 408,563 - +1.3 408,592 + -0.3 408,654 + +0.2 408,772 - +2.0 408,880 + +1.6 408,939 + +0.1 409,009 - -1.4 409,030 + -0.9 409,035 + +0.1 409,038 - -0.7 409,043 - -0.2 409,049 + -1.7 409,049 + +0.3 409,062 - +0.3 409,062 - +0.5 409,088 + -0.8 409,088 + -0.1 409,088 + +0.8 409,088 + +0.2 409,096 - -0.4 409,102 + -1.6 409,102 + +0.1 409,102 + -0.2 409,102 + -0.5 409,110 - +0.0 409,110 - -0.2 409,110 - -0.2 409,110 - -0.7 409,110 - -0.3 409,137 + -0.5 409,180 + +1.2 409,180 + -0.4 409,184 + +1.0 409,194 - +0.6 409,204 + -1.9 409,217 + +0.3 409,222 + +0.6 409,235 - -0.9 409,329 - BWI76_RS01990 0.12 -0.5 409,374 + BWI76_RS01990 0.15 +0.9 409,381 - BWI76_RS01990 0.15 -1.5 409,404 + BWI76_RS01990 0.17 -2.3 409,412 - BWI76_RS01990 0.17 -1.0 409,412 - BWI76_RS01990 0.17 -0.6 409,417 + BWI76_RS01990 0.18 +0.3 409,571 + BWI76_RS01990 0.27 +0.8 409,571 + BWI76_RS01990 0.27 +1.9 409,579 - BWI76_RS01990 0.28 +2.3 409,579 - BWI76_RS01990 0.28 +0.4 409,579 - BWI76_RS01990 0.28 +0.2 409,579 - BWI76_RS01990 0.28 +0.6 409,579 - BWI76_RS01990 0.28 -0.2
Or see this region's nucleotide sequence