Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01100 and BWI76_RS01105 are separated by 18 nucleotides BWI76_RS01105 and BWI76_RS01110 overlap by 23 nucleotides BWI76_RS01110 and BWI76_RS01115 are separated by 4 nucleotides
BWI76_RS01100: BWI76_RS01100 - dTDP-glucose 4,6-dehydratase, at 226,819 to 227,887
_RS01100
BWI76_RS01105: BWI76_RS01105 - glucose-1-phosphate thymidylyltransferase, at 227,906 to 228,787
_RS01105
BWI76_RS01110: BWI76_RS01110 - TDP-fucosamine acetyltransferase, at 228,765 to 229,439
_RS01110
BWI76_RS01115: BWI76_RS01115 - dTDP-4-amino-4,6-dideoxygalactose transaminase, at 229,444 to 230,574
_RS01115
Position (kb) 228
229
230 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 227.921 kb on - strand at 228.196 kb on - strand, within BWI76_RS01105 at 228.249 kb on + strand, within BWI76_RS01105 at 228.263 kb on - strand, within BWI76_RS01105 at 228.273 kb on - strand, within BWI76_RS01105 at 228.384 kb on + strand, within BWI76_RS01105 at 228.654 kb on + strand, within BWI76_RS01105 at 228.789 kb on - strand at 228.839 kb on - strand, within BWI76_RS01110 at 228.927 kb on + strand, within BWI76_RS01110 at 229.276 kb on - strand, within BWI76_RS01110 at 229.279 kb on + strand, within BWI76_RS01110 at 229.315 kb on + strand, within BWI76_RS01110 at 229.536 kb on - strand at 229.602 kb on + strand, within BWI76_RS01115 at 229.711 kb on - strand, within BWI76_RS01115 at 229.716 kb on - strand, within BWI76_RS01115
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 227,921 - +2.1 228,196 - BWI76_RS01105 0.33 -3.8 228,249 + BWI76_RS01105 0.39 -1.0 228,263 - BWI76_RS01105 0.40 -2.4 228,273 - BWI76_RS01105 0.42 -2.8 228,384 + BWI76_RS01105 0.54 -1.1 228,654 + BWI76_RS01105 0.85 -3.9 228,789 - -2.9 228,839 - BWI76_RS01110 0.11 -2.6 228,927 + BWI76_RS01110 0.24 +0.1 229,276 - BWI76_RS01110 0.76 +0.2 229,279 + BWI76_RS01110 0.76 -3.9 229,315 + BWI76_RS01110 0.81 -3.2 229,536 - -3.6 229,602 + BWI76_RS01115 0.14 -3.0 229,711 - BWI76_RS01115 0.24 -1.8 229,716 - BWI76_RS01115 0.24 -4.1
Or see this region's nucleotide sequence