Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01095 and BWI76_RS01100 overlap by 4 nucleotides BWI76_RS01100 and BWI76_RS01105 are separated by 18 nucleotides BWI76_RS01105 and BWI76_RS01110 overlap by 23 nucleotides
BWI76_RS01095: BWI76_RS01095 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 225,560 to 226,822
_RS01095
BWI76_RS01100: BWI76_RS01100 - dTDP-glucose 4,6-dehydratase, at 226,819 to 227,887
_RS01100
BWI76_RS01105: BWI76_RS01105 - glucose-1-phosphate thymidylyltransferase, at 227,906 to 228,787
_RS01105
BWI76_RS01110: BWI76_RS01110 - TDP-fucosamine acetyltransferase, at 228,765 to 229,439
_RS01110
Position (kb) 226
227
228 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 226.034 kb on - strand, within BWI76_RS01095 at 226.533 kb on + strand, within BWI76_RS01095 at 226.541 kb on - strand, within BWI76_RS01095 at 226.711 kb on + strand at 226.776 kb on + strand at 226.901 kb on + strand at 226.918 kb on + strand at 226.979 kb on + strand, within BWI76_RS01100 at 227.132 kb on + strand, within BWI76_RS01100 at 227.132 kb on + strand, within BWI76_RS01100 at 227.132 kb on + strand, within BWI76_RS01100 at 227.140 kb on - strand, within BWI76_RS01100 at 227.142 kb on + strand, within BWI76_RS01100 at 227.227 kb on + strand, within BWI76_RS01100 at 227.235 kb on - strand, within BWI76_RS01100 at 227.642 kb on - strand, within BWI76_RS01100 at 227.921 kb on - strand at 228.196 kb on - strand, within BWI76_RS01105 at 228.249 kb on + strand, within BWI76_RS01105 at 228.263 kb on - strand, within BWI76_RS01105 at 228.273 kb on - strand, within BWI76_RS01105 at 228.384 kb on + strand, within BWI76_RS01105 at 228.654 kb on + strand, within BWI76_RS01105 at 228.789 kb on - strand at 228.839 kb on - strand, within BWI76_RS01110
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 226,034 - BWI76_RS01095 0.38 -0.3 226,533 + BWI76_RS01095 0.77 -1.8 226,541 - BWI76_RS01095 0.78 -3.3 226,711 + -1.2 226,776 + -2.8 226,901 + -0.1 226,918 + -1.7 226,979 + BWI76_RS01100 0.15 -1.9 227,132 + BWI76_RS01100 0.29 -1.3 227,132 + BWI76_RS01100 0.29 +1.9 227,132 + BWI76_RS01100 0.29 -3.1 227,140 - BWI76_RS01100 0.30 -3.7 227,142 + BWI76_RS01100 0.30 -2.2 227,227 + BWI76_RS01100 0.38 -0.4 227,235 - BWI76_RS01100 0.39 -2.4 227,642 - BWI76_RS01100 0.77 -3.9 227,921 - +2.1 228,196 - BWI76_RS01105 0.33 -3.8 228,249 + BWI76_RS01105 0.39 -1.0 228,263 - BWI76_RS01105 0.40 -2.4 228,273 - BWI76_RS01105 0.42 -2.8 228,384 + BWI76_RS01105 0.54 -1.1 228,654 + BWI76_RS01105 0.85 -3.9 228,789 - -2.9 228,839 - BWI76_RS01110 0.11 -2.6
Or see this region's nucleotide sequence