Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01060 and BWI76_RS01065 are separated by 134 nucleotides BWI76_RS01065 and BWI76_RS01070 are separated by 341 nucleotides
BWI76_RS01060: BWI76_RS01060 - guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase, at 216,812 to 218,311
_RS01060
BWI76_RS01065: BWI76_RS01065 - ATP-dependent RNA helicase RhlB, at 218,446 to 219,711
_RS01065
BWI76_RS01070: BWI76_RS01070 - thiol reductase thioredoxin, at 220,053 to 220,382
_RS01070
Position (kb) 218
219
220 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 217.717 kb on + strand, within BWI76_RS01060 at 217.720 kb on - strand, within BWI76_RS01060 at 217.835 kb on - strand, within BWI76_RS01060 at 217.993 kb on - strand, within BWI76_RS01060 at 218.297 kb on - strand at 218.299 kb on + strand at 218.321 kb on - strand at 218.332 kb on + strand at 218.340 kb on - strand at 218.448 kb on + strand at 218.451 kb on - strand at 218.533 kb on - strand at 218.533 kb on - strand at 218.965 kb on + strand, within BWI76_RS01065 at 218.966 kb on + strand, within BWI76_RS01065 at 219.065 kb on + strand, within BWI76_RS01065 at 219.124 kb on - strand, within BWI76_RS01065 at 219.124 kb on - strand, within BWI76_RS01065 at 219.254 kb on - strand, within BWI76_RS01065 at 219.323 kb on + strand, within BWI76_RS01065 at 219.377 kb on - strand, within BWI76_RS01065 at 219.742 kb on - strand at 219.863 kb on + strand at 219.901 kb on + strand at 220.103 kb on + strand, within BWI76_RS01070 at 220.103 kb on + strand, within BWI76_RS01070 at 220.103 kb on + strand, within BWI76_RS01070 at 220.111 kb on - strand, within BWI76_RS01070 at 220.125 kb on - strand, within BWI76_RS01070 at 220.125 kb on - strand, within BWI76_RS01070 at 220.155 kb on - strand, within BWI76_RS01070 at 220.226 kb on + strand, within BWI76_RS01070 at 220.337 kb on + strand, within BWI76_RS01070 at 220.344 kb on + strand, within BWI76_RS01070 at 220.345 kb on - strand, within BWI76_RS01070 at 220.345 kb on - strand, within BWI76_RS01070 at 220.414 kb on - strand at 220.433 kb on - strand at 220.448 kb on + strand at 220.504 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 217,717 + BWI76_RS01060 0.60 -1.0 217,720 - BWI76_RS01060 0.61 +1.1 217,835 - BWI76_RS01060 0.68 -0.4 217,993 - BWI76_RS01060 0.79 -1.2 218,297 - -1.6 218,299 + -2.0 218,321 - -1.2 218,332 + -1.6 218,340 - +0.1 218,448 + -1.2 218,451 - -0.6 218,533 - -0.1 218,533 - -2.5 218,965 + BWI76_RS01065 0.41 -1.3 218,966 + BWI76_RS01065 0.41 -2.2 219,065 + BWI76_RS01065 0.49 -1.2 219,124 - BWI76_RS01065 0.54 -1.1 219,124 - BWI76_RS01065 0.54 -0.3 219,254 - BWI76_RS01065 0.64 -1.7 219,323 + BWI76_RS01065 0.69 -0.9 219,377 - BWI76_RS01065 0.74 -1.5 219,742 - -1.8 219,863 + -3.0 219,901 + -1.2 220,103 + BWI76_RS01070 0.15 -1.3 220,103 + BWI76_RS01070 0.15 -2.3 220,103 + BWI76_RS01070 0.15 -2.8 220,111 - BWI76_RS01070 0.18 +0.5 220,125 - BWI76_RS01070 0.22 -1.7 220,125 - BWI76_RS01070 0.22 -0.6 220,155 - BWI76_RS01070 0.31 -0.5 220,226 + BWI76_RS01070 0.52 -1.7 220,337 + BWI76_RS01070 0.86 -0.3 220,344 + BWI76_RS01070 0.88 -1.6 220,345 - BWI76_RS01070 0.88 -3.0 220,345 - BWI76_RS01070 0.88 -1.8 220,414 - -3.2 220,433 - -0.5 220,448 + -1.7 220,504 + -1.1
Or see this region's nucleotide sequence