Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00875 and BWI76_RS00880 are separated by 15 nucleotides BWI76_RS00880 and BWI76_RS00885 are separated by 46 nucleotides BWI76_RS00885 and BWI76_RS00890 are separated by 364 nucleotides
BWI76_RS00875: BWI76_RS00875 - DNA-binding transcriptional regulator FabR, at 180,927 to 181,562
_RS00875
BWI76_RS00880: BWI76_RS00880 - membrane protein, at 181,578 to 181,937
_RS00880
BWI76_RS00885: BWI76_RS00885 - tRNA (uridine(54)-C5)-methyltransferase TrmA, at 181,984 to 183,084
_RS00885
BWI76_RS00890: BWI76_RS00890 - vitamin B12/cobalamin outer membrane transporter, at 183,449 to 185,317
_RS00890
Position (kb) 181
182
183
184 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 181.021 kb on + strand, within BWI76_RS00875 at 181.021 kb on + strand, within BWI76_RS00875 at 181.021 kb on + strand, within BWI76_RS00875 at 181.109 kb on + strand, within BWI76_RS00875 at 181.129 kb on + strand, within BWI76_RS00875 at 181.294 kb on + strand, within BWI76_RS00875 at 181.383 kb on + strand, within BWI76_RS00875 at 181.388 kb on + strand, within BWI76_RS00875 at 181.396 kb on - strand, within BWI76_RS00875 at 181.444 kb on + strand, within BWI76_RS00875 at 181.586 kb on - strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.590 kb on + strand at 181.595 kb on + strand at 181.595 kb on + strand at 181.598 kb on - strand at 181.603 kb on - strand at 181.659 kb on + strand, within BWI76_RS00880 at 181.661 kb on + strand, within BWI76_RS00880 at 181.661 kb on + strand, within BWI76_RS00880 at 181.886 kb on + strand, within BWI76_RS00880 at 182.436 kb on + strand, within BWI76_RS00885 at 182.439 kb on - strand, within BWI76_RS00885 at 182.575 kb on - strand, within BWI76_RS00885 at 182.637 kb on + strand, within BWI76_RS00885 at 182.713 kb on + strand, within BWI76_RS00885 at 182.772 kb on + strand, within BWI76_RS00885 at 182.812 kb on - strand, within BWI76_RS00885 at 183.052 kb on + strand at 183.062 kb on + strand at 183.078 kb on - strand at 183.079 kb on + strand at 183.121 kb on - strand at 183.129 kb on + strand at 183.140 kb on + strand at 183.140 kb on + strand at 183.140 kb on + strand at 183.145 kb on + strand at 183.231 kb on + strand at 183.236 kb on + strand at 183.290 kb on - strand at 183.328 kb on + strand at 183.354 kb on - strand at 183.360 kb on - strand at 183.413 kb on - strand at 183.419 kb on - strand at 183.428 kb on + strand at 183.527 kb on - strand at 183.535 kb on + strand at 183.536 kb on - strand at 183.567 kb on + strand at 183.613 kb on - strand at 183.753 kb on - strand, within BWI76_RS00890 at 183.758 kb on + strand, within BWI76_RS00890 at 183.786 kb on + strand, within BWI76_RS00890 at 183.830 kb on + strand, within BWI76_RS00890 at 183.872 kb on + strand, within BWI76_RS00890 at 183.880 kb on - strand, within BWI76_RS00890 at 183.894 kb on - strand, within BWI76_RS00890 at 183.942 kb on + strand, within BWI76_RS00890 at 183.942 kb on + strand, within BWI76_RS00890 at 183.942 kb on + strand, within BWI76_RS00890 at 183.945 kb on - strand, within BWI76_RS00890 at 183.951 kb on - strand, within BWI76_RS00890 at 184.005 kb on - strand, within BWI76_RS00890 at 184.017 kb on + strand, within BWI76_RS00890 at 184.017 kb on + strand, within BWI76_RS00890 at 184.066 kb on + strand, within BWI76_RS00890
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 181,021 + BWI76_RS00875 0.15 -0.6 181,021 + BWI76_RS00875 0.15 -0.2 181,021 + BWI76_RS00875 0.15 +0.4 181,109 + BWI76_RS00875 0.29 +0.2 181,129 + BWI76_RS00875 0.32 +0.7 181,294 + BWI76_RS00875 0.58 +0.9 181,383 + BWI76_RS00875 0.72 -0.2 181,388 + BWI76_RS00875 0.72 -0.3 181,396 - BWI76_RS00875 0.74 +1.7 181,444 + BWI76_RS00875 0.81 -0.4 181,586 - +1.3 181,590 + +0.6 181,590 + +0.8 181,590 + +0.4 181,590 + -0.4 181,590 + +1.8 181,595 + +0.2 181,595 + +0.1 181,598 - +1.9 181,603 - -0.4 181,659 + BWI76_RS00880 0.23 +0.2 181,661 + BWI76_RS00880 0.23 +0.0 181,661 + BWI76_RS00880 0.23 -2.2 181,886 + BWI76_RS00880 0.86 +1.4 182,436 + BWI76_RS00885 0.41 -0.6 182,439 - BWI76_RS00885 0.41 -1.2 182,575 - BWI76_RS00885 0.54 -0.2 182,637 + BWI76_RS00885 0.59 -0.9 182,713 + BWI76_RS00885 0.66 -0.5 182,772 + BWI76_RS00885 0.72 -0.9 182,812 - BWI76_RS00885 0.75 -1.4 183,052 + -0.8 183,062 + -1.2 183,078 - +1.0 183,079 + +3.6 183,121 - -0.8 183,129 + -0.8 183,140 + -1.9 183,140 + -0.4 183,140 + -2.3 183,145 + -0.0 183,231 + -0.7 183,236 + -0.1 183,290 - -1.1 183,328 + +0.0 183,354 - -1.1 183,360 - -0.3 183,413 - +0.1 183,419 - +0.2 183,428 + +0.2 183,527 - +0.2 183,535 + -0.4 183,536 - +0.1 183,567 + -0.6 183,613 - -0.0 183,753 - BWI76_RS00890 0.16 +0.9 183,758 + BWI76_RS00890 0.17 -0.8 183,786 + BWI76_RS00890 0.18 -0.6 183,830 + BWI76_RS00890 0.20 -0.2 183,872 + BWI76_RS00890 0.23 -1.0 183,880 - BWI76_RS00890 0.23 -0.5 183,894 - BWI76_RS00890 0.24 -0.2 183,942 + BWI76_RS00890 0.26 +0.2 183,942 + BWI76_RS00890 0.26 +0.3 183,942 + BWI76_RS00890 0.26 +0.2 183,945 - BWI76_RS00890 0.27 +0.5 183,951 - BWI76_RS00890 0.27 -1.4 184,005 - BWI76_RS00890 0.30 +1.0 184,017 + BWI76_RS00890 0.30 +0.5 184,017 + BWI76_RS00890 0.30 -0.2 184,066 + BWI76_RS00890 0.33 +0.1
Or see this region's nucleotide sequence