Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00855 and BWI76_RS00860 are separated by 31 nucleotides BWI76_RS00860 and BWI76_RS00865 overlap by 1 nucleotides BWI76_RS00865 and BWI76_RS00870 overlap by 18 nucleotides
BWI76_RS00855: BWI76_RS00855 - argininosuccinate lyase, at 176,786 to 178,159
_RS00855
BWI76_RS00860: BWI76_RS00860 - hypothetical protein, at 178,191 to 178,430
_RS00860
BWI76_RS00865: BWI76_RS00865 - DNA-binding transcriptional regulator OxyR, at 178,430 to 179,347
_RS00865
BWI76_RS00870: BWI76_RS00870 - NAD(P)(+) transhydrogenase, at 179,330 to 180,730
_RS00870
Position (kb) 178
179
180 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 177.458 kb on + strand, within BWI76_RS00855 at 177.466 kb on - strand, within BWI76_RS00855 at 177.598 kb on + strand, within BWI76_RS00855 at 177.712 kb on + strand, within BWI76_RS00855 at 177.719 kb on - strand, within BWI76_RS00855 at 177.744 kb on - strand, within BWI76_RS00855 at 178.145 kb on - strand at 178.161 kb on - strand at 178.161 kb on - strand at 178.175 kb on + strand at 178.551 kb on - strand, within BWI76_RS00865 at 178.618 kb on - strand, within BWI76_RS00865 at 178.761 kb on - strand, within BWI76_RS00865 at 178.943 kb on + strand, within BWI76_RS00865 at 179.153 kb on + strand, within BWI76_RS00865 at 179.208 kb on - strand, within BWI76_RS00865 at 179.279 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 177,458 + BWI76_RS00855 0.49 -0.2 177,466 - BWI76_RS00855 0.49 +0.5 177,598 + BWI76_RS00855 0.59 +0.6 177,712 + BWI76_RS00855 0.67 +2.6 177,719 - BWI76_RS00855 0.68 +0.7 177,744 - BWI76_RS00855 0.70 +0.1 178,145 - -0.0 178,161 - +0.7 178,161 - -1.1 178,175 + -0.9 178,551 - BWI76_RS00865 0.13 -2.7 178,618 - BWI76_RS00865 0.20 -0.9 178,761 - BWI76_RS00865 0.36 -3.5 178,943 + BWI76_RS00865 0.56 -2.4 179,153 + BWI76_RS00865 0.79 -3.5 179,208 - BWI76_RS00865 0.85 -2.5 179,279 - -1.8
Or see this region's nucleotide sequence