Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00315 and BWI76_RS00320 are separated by 58 nucleotides BWI76_RS00320 and BWI76_RS00325 are separated by 124 nucleotides BWI76_RS00325 and BWI76_RS00330 overlap by 4 nucleotides BWI76_RS00330 and BWI76_RS00335 are separated by 71 nucleotides BWI76_RS00335 and BWI76_RS00340 are separated by 75 nucleotides
BWI76_RS00315: BWI76_RS00315 - 23S ribosomal RNA, at 66,486 to 69,413
_RS00315
BWI76_RS00320: BWI76_RS00320 - 5S ribosomal RNA, at 69,472 to 69,587
_RS00320
BWI76_RS00325: BWI76_RS00325 - molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 69,712 to 70,218
_RS00325
BWI76_RS00330: BWI76_RS00330 - molybdenum cofactor guanylyltransferase, at 70,215 to 70,796
_RS00330
BWI76_RS00335: BWI76_RS00335 - hypothetical protein, at 70,868 to 71,137
_RS00335
BWI76_RS00340: BWI76_RS00340 - stress response kinase A, at 71,213 to 72,199
_RS00340
Position (kb) 70
71 Strain fitness (log2 ratio) -2
-1
0
1
2 at 69.863 kb on - strand, within BWI76_RS00325 at 69.926 kb on - strand, within BWI76_RS00325 at 70.056 kb on + strand, within BWI76_RS00325 at 70.124 kb on - strand, within BWI76_RS00325 at 70.364 kb on - strand, within BWI76_RS00330 at 70.386 kb on + strand, within BWI76_RS00330 at 70.525 kb on + strand, within BWI76_RS00330 at 70.791 kb on - strand at 70.829 kb on - strand at 70.835 kb on + strand at 70.844 kb on - strand at 70.913 kb on + strand, within BWI76_RS00335 at 71.051 kb on - strand, within BWI76_RS00335 at 71.115 kb on - strand at 71.188 kb on + strand at 71.224 kb on + strand at 71.232 kb on - strand at 71.303 kb on - strand at 71.317 kb on - strand, within BWI76_RS00340 at 71.529 kb on + strand, within BWI76_RS00340 at 71.637 kb on - strand, within BWI76_RS00340
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 69,863 - BWI76_RS00325 0.30 -0.1 69,926 - BWI76_RS00325 0.42 +0.2 70,056 + BWI76_RS00325 0.68 -1.9 70,124 - BWI76_RS00325 0.81 -0.3 70,364 - BWI76_RS00330 0.26 +2.2 70,386 + BWI76_RS00330 0.29 +1.7 70,525 + BWI76_RS00330 0.53 +2.2 70,791 - -1.4 70,829 - -0.0 70,835 + -0.3 70,844 - +0.5 70,913 + BWI76_RS00335 0.17 +0.5 71,051 - BWI76_RS00335 0.68 -1.1 71,115 - +0.2 71,188 + +0.3 71,224 + +0.1 71,232 - +0.2 71,303 - +0.5 71,317 - BWI76_RS00340 0.11 +0.5 71,529 + BWI76_RS00340 0.32 +0.4 71,637 - BWI76_RS00340 0.43 +0.3
Or see this region's nucleotide sequence