Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00290 and BWI76_RS00295 are separated by 12 nucleotides BWI76_RS00295 and BWI76_RS00300 overlap by 4 nucleotides
BWI76_RS00290: BWI76_RS00290 - Ribokinase (EC 2.7.1.15) (from data) , at 60,235 to 61,164
_RS00290
BWI76_RS00295: BWI76_RS00295 - transcriptional regulator RbsR, at 61,177 to 62,160
_RS00295
BWI76_RS00300: BWI76_RS00300 - MFS transporter, at 62,157 to 63,554
_RS00300
Position (kb)
61
62
63 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 60.261 kb on - strand at 60.289 kb on + strand at 60.371 kb on + strand, within BWI76_RS00290 at 60.462 kb on + strand, within BWI76_RS00290 at 60.500 kb on - strand, within BWI76_RS00290 at 60.505 kb on - strand, within BWI76_RS00290 at 60.519 kb on - strand, within BWI76_RS00290 at 60.597 kb on - strand at 60.599 kb on - strand, within BWI76_RS00290 at 60.635 kb on + strand, within BWI76_RS00290 at 60.656 kb on + strand, within BWI76_RS00290 at 60.734 kb on - strand, within BWI76_RS00290 at 60.734 kb on - strand, within BWI76_RS00290 at 60.793 kb on + strand, within BWI76_RS00290 at 60.820 kb on - strand, within BWI76_RS00290 at 60.821 kb on - strand, within BWI76_RS00290 at 60.865 kb on - strand, within BWI76_RS00290 at 61.113 kb on - strand at 61.165 kb on + strand at 61.173 kb on - strand at 61.431 kb on + strand, within BWI76_RS00295 at 61.433 kb on + strand, within BWI76_RS00295 at 61.439 kb on + strand, within BWI76_RS00295 at 61.441 kb on - strand, within BWI76_RS00295 at 61.441 kb on - strand, within BWI76_RS00295 at 61.447 kb on - strand, within BWI76_RS00295 at 61.457 kb on - strand, within BWI76_RS00295 at 61.580 kb on - strand, within BWI76_RS00295 at 61.960 kb on + strand, within BWI76_RS00295 at 61.960 kb on + strand, within BWI76_RS00295 at 61.968 kb on - strand, within BWI76_RS00295 at 62.007 kb on + strand, within BWI76_RS00295 at 62.102 kb on + strand at 62.115 kb on - strand at 62.137 kb on - strand at 62.143 kb on - strand at 62.163 kb on - strand at 62.169 kb on - strand at 62.273 kb on + strand at 62.429 kb on + strand, within BWI76_RS00300 at 62.470 kb on + strand, within BWI76_RS00300 at 62.584 kb on - strand, within BWI76_RS00300 at 62.599 kb on - strand, within BWI76_RS00300 at 62.633 kb on + strand, within BWI76_RS00300 at 62.673 kb on + strand, within BWI76_RS00300 at 62.730 kb on - strand, within BWI76_RS00300 at 62.814 kb on - strand, within BWI76_RS00300 at 63.029 kb on - strand, within BWI76_RS00300 at 63.075 kb on + strand, within BWI76_RS00300 at 63.088 kb on - strand, within BWI76_RS00300 at 63.098 kb on - strand, within BWI76_RS00300 at 63.110 kb on - strand, within BWI76_RS00300 at 63.115 kb on - strand, within BWI76_RS00300 at 63.129 kb on - strand, within BWI76_RS00300 at 63.129 kb on - strand, within BWI76_RS00300 at 63.130 kb on - strand, within BWI76_RS00300
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 60,261 - +1.3 60,289 + +1.3 60,371 + BWI76_RS00290 0.15 +0.8 60,462 + BWI76_RS00290 0.24 +0.2 60,500 - BWI76_RS00290 0.28 +2.0 60,505 - BWI76_RS00290 0.29 +0.7 60,519 - BWI76_RS00290 0.31 -0.6 60,597 - +3.0 60,599 - BWI76_RS00290 0.39 -0.2 60,635 + BWI76_RS00290 0.43 +0.2 60,656 + BWI76_RS00290 0.45 +0.3 60,734 - BWI76_RS00290 0.54 +0.7 60,734 - BWI76_RS00290 0.54 +0.3 60,793 + BWI76_RS00290 0.60 -0.1 60,820 - BWI76_RS00290 0.63 -0.3 60,821 - BWI76_RS00290 0.63 -0.3 60,865 - BWI76_RS00290 0.68 +0.7 61,113 - +0.3 61,165 + +1.6 61,173 - +1.2 61,431 + BWI76_RS00295 0.26 +0.8 61,433 + BWI76_RS00295 0.26 +1.4 61,439 + BWI76_RS00295 0.27 +1.3 61,441 - BWI76_RS00295 0.27 -2.1 61,441 - BWI76_RS00295 0.27 +1.6 61,447 - BWI76_RS00295 0.27 -0.3 61,457 - BWI76_RS00295 0.28 +1.3 61,580 - BWI76_RS00295 0.41 +0.9 61,960 + BWI76_RS00295 0.80 +1.2 61,960 + BWI76_RS00295 0.80 +1.0 61,968 - BWI76_RS00295 0.80 +0.8 62,007 + BWI76_RS00295 0.84 +0.6 62,102 + +1.0 62,115 - +0.7 62,137 - +1.1 62,143 - +0.5 62,163 - +0.1 62,169 - -0.2 62,273 + +0.3 62,429 + BWI76_RS00300 0.19 -0.2 62,470 + BWI76_RS00300 0.22 -0.1 62,584 - BWI76_RS00300 0.31 -0.5 62,599 - BWI76_RS00300 0.32 -0.5 62,633 + BWI76_RS00300 0.34 -0.2 62,673 + BWI76_RS00300 0.37 +0.8 62,730 - BWI76_RS00300 0.41 -0.4 62,814 - BWI76_RS00300 0.47 +0.2 63,029 - BWI76_RS00300 0.62 +2.6 63,075 + BWI76_RS00300 0.66 +0.4 63,088 - BWI76_RS00300 0.67 -0.9 63,098 - BWI76_RS00300 0.67 -0.2 63,110 - BWI76_RS00300 0.68 +0.2 63,115 - BWI76_RS00300 0.69 -0.9 63,129 - BWI76_RS00300 0.70 +0.3 63,129 - BWI76_RS00300 0.70 -0.9 63,130 - BWI76_RS00300 0.70 +1.3
Or see this region's nucleotide sequence