Experiment: Sisomicin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00220 and BWI76_RS00225 are separated by 616 nucleotides BWI76_RS00225 and BWI76_RS00230 are separated by 112 nucleotides
BWI76_RS00220: BWI76_RS00220 - F0F1 ATP synthase subunit I, at 44,948 to 45,328
_RS00220
BWI76_RS00225: BWI76_RS00225 - 16S rRNA methyltransferase G, at 45,945 to 46,568
_RS00225
BWI76_RS00230: BWI76_RS00230 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 46,681 to 48,570
_RS00230
Position (kb) 45
46
47 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 45.090 kb on - strand, within BWI76_RS00220 at 45.115 kb on - strand, within BWI76_RS00220 at 45.518 kb on - strand at 45.684 kb on + strand at 45.818 kb on - strand at 45.858 kb on + strand at 45.968 kb on + strand at 45.968 kb on + strand at 45.981 kb on - strand at 45.985 kb on + strand at 45.985 kb on + strand at 45.985 kb on + strand at 45.993 kb on - strand at 45.997 kb on + strand at 46.005 kb on - strand at 46.064 kb on + strand, within BWI76_RS00225 at 46.064 kb on + strand, within BWI76_RS00225 at 46.077 kb on - strand, within BWI76_RS00225 at 46.203 kb on + strand, within BWI76_RS00225 at 46.208 kb on + strand, within BWI76_RS00225 at 46.211 kb on - strand, within BWI76_RS00225 at 46.211 kb on - strand, within BWI76_RS00225 at 46.271 kb on + strand, within BWI76_RS00225 at 46.279 kb on - strand, within BWI76_RS00225 at 46.290 kb on + strand, within BWI76_RS00225 at 46.337 kb on + strand, within BWI76_RS00225 at 46.372 kb on + strand, within BWI76_RS00225 at 46.380 kb on - strand, within BWI76_RS00225 at 46.427 kb on + strand, within BWI76_RS00225 at 46.427 kb on + strand, within BWI76_RS00225 at 46.471 kb on - strand, within BWI76_RS00225 at 46.523 kb on + strand at 46.531 kb on - strand at 46.578 kb on - strand at 46.595 kb on + strand at 46.641 kb on + strand at 46.681 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.008 mg/ml remove 45,090 - BWI76_RS00220 0.37 -1.4 45,115 - BWI76_RS00220 0.44 -0.8 45,518 - -0.4 45,684 + -2.4 45,818 - -2.2 45,858 + -2.8 45,968 + -2.3 45,968 + -1.5 45,981 - -0.0 45,985 + -1.6 45,985 + -2.2 45,985 + -0.4 45,993 - -2.2 45,997 + -1.9 46,005 - -3.1 46,064 + BWI76_RS00225 0.19 -2.0 46,064 + BWI76_RS00225 0.19 -1.5 46,077 - BWI76_RS00225 0.21 -1.4 46,203 + BWI76_RS00225 0.41 -4.1 46,208 + BWI76_RS00225 0.42 -1.2 46,211 - BWI76_RS00225 0.43 -1.7 46,211 - BWI76_RS00225 0.43 -1.0 46,271 + BWI76_RS00225 0.52 -0.9 46,279 - BWI76_RS00225 0.54 -0.9 46,290 + BWI76_RS00225 0.55 -2.0 46,337 + BWI76_RS00225 0.63 -2.9 46,372 + BWI76_RS00225 0.68 -2.3 46,380 - BWI76_RS00225 0.70 -2.0 46,427 + BWI76_RS00225 0.77 -2.2 46,427 + BWI76_RS00225 0.77 -0.9 46,471 - BWI76_RS00225 0.84 +0.5 46,523 + +0.8 46,531 - -0.4 46,578 - -0.7 46,595 + -1.3 46,641 + -1.2 46,681 - -1.0
Or see this region's nucleotide sequence