Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_02020

Experiment: N1B4wt with N2E3m denitrifying replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_02010 and AO353_02015 overlap by 17 nucleotidesAO353_02015 and AO353_02020 are separated by 170 nucleotidesAO353_02020 and AO353_02025 are separated by 98 nucleotides AO353_02010: AO353_02010 - glycosyl transferase family 1, at 428,473 to 429,720 _02010 AO353_02015: AO353_02015 - acetyltransferase, at 429,704 to 430,333 _02015 AO353_02020: AO353_02020 - hypothetical protein, at 430,504 to 431,469 _02020 AO353_02025: AO353_02025 - mannose-1-phosphate guanyltransferase, at 431,568 to 433,028 _02025 Position (kb) 430 431 432Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 429.508 kb on + strand, within AO353_02010at 429.508 kb on + strand, within AO353_02010at 429.508 kb on + strand, within AO353_02010at 429.508 kb on + strand, within AO353_02010at 429.510 kb on + strand, within AO353_02010at 429.537 kb on - strand, within AO353_02010at 429.590 kb on + strand, within AO353_02010at 429.631 kb on - strandat 429.642 kb on - strandat 429.642 kb on - strandat 429.642 kb on - strandat 429.642 kb on - strandat 429.642 kb on - strandat 429.715 kb on + strandat 429.767 kb on - strand, within AO353_02015at 429.771 kb on + strand, within AO353_02015at 429.771 kb on + strand, within AO353_02015at 429.772 kb on - strand, within AO353_02015at 429.887 kb on + strand, within AO353_02015at 429.887 kb on + strand, within AO353_02015at 429.888 kb on - strand, within AO353_02015at 429.888 kb on - strand, within AO353_02015at 429.893 kb on + strand, within AO353_02015at 429.893 kb on + strand, within AO353_02015at 429.894 kb on - strand, within AO353_02015at 429.894 kb on - strand, within AO353_02015at 429.982 kb on + strand, within AO353_02015at 429.982 kb on + strand, within AO353_02015at 430.005 kb on - strand, within AO353_02015at 430.042 kb on + strand, within AO353_02015at 430.068 kb on + strand, within AO353_02015at 430.069 kb on - strand, within AO353_02015at 430.069 kb on - strand, within AO353_02015at 430.070 kb on + strand, within AO353_02015at 430.070 kb on + strand, within AO353_02015at 430.070 kb on + strand, within AO353_02015at 430.070 kb on + strand, within AO353_02015at 430.070 kb on + strand, within AO353_02015at 430.071 kb on - strand, within AO353_02015at 430.071 kb on - strand, within AO353_02015at 430.071 kb on - strand, within AO353_02015at 430.071 kb on - strand, within AO353_02015at 430.071 kb on - strand, within AO353_02015at 430.071 kb on - strand, within AO353_02015at 430.072 kb on + strand, within AO353_02015at 430.073 kb on - strand, within AO353_02015at 430.073 kb on - strand, within AO353_02015at 430.082 kb on - strand, within AO353_02015at 430.094 kb on - strand, within AO353_02015at 430.159 kb on + strand, within AO353_02015at 430.254 kb on + strand, within AO353_02015at 430.275 kb on + strandat 430.275 kb on + strandat 430.275 kb on + strandat 430.275 kb on + strandat 430.276 kb on - strandat 430.276 kb on - strandat 430.276 kb on - strandat 430.276 kb on - strandat 430.278 kb on + strandat 430.278 kb on + strandat 430.278 kb on + strandat 430.279 kb on - strandat 430.324 kb on + strandat 430.324 kb on + strandat 430.325 kb on - strandat 430.372 kb on + strandat 430.373 kb on - strandat 430.373 kb on - strandat 430.396 kb on + strandat 430.396 kb on + strandat 430.396 kb on + strandat 430.420 kb on + strandat 430.450 kb on + strandat 430.450 kb on + strandat 430.464 kb on + strandat 430.464 kb on + strandat 430.464 kb on + strandat 430.464 kb on + strandat 430.464 kb on + strandat 430.465 kb on - strandat 430.465 kb on - strandat 430.505 kb on + strandat 430.505 kb on + strandat 430.505 kb on + strandat 430.516 kb on + strandat 430.632 kb on + strand, within AO353_02020at 430.632 kb on + strand, within AO353_02020at 430.702 kb on - strand, within AO353_02020at 430.748 kb on - strand, within AO353_02020at 430.786 kb on - strand, within AO353_02020at 430.862 kb on + strand, within AO353_02020at 430.862 kb on + strand, within AO353_02020at 430.863 kb on - strand, within AO353_02020at 430.951 kb on - strand, within AO353_02020at 431.064 kb on - strand, within AO353_02020at 431.064 kb on - strand, within AO353_02020at 431.133 kb on + strand, within AO353_02020at 431.159 kb on + strand, within AO353_02020at 431.159 kb on + strand, within AO353_02020at 431.189 kb on - strand, within AO353_02020at 431.189 kb on - strand, within AO353_02020at 431.189 kb on - strand, within AO353_02020at 431.231 kb on + strand, within AO353_02020at 431.231 kb on + strand, within AO353_02020at 431.232 kb on - strand, within AO353_02020at 431.232 kb on - strand, within AO353_02020at 431.375 kb on + strandat 431.407 kb on - strandat 431.452 kb on - strandat 431.452 kb on - strandat 431.455 kb on + strandat 431.456 kb on - strandat 431.495 kb on + strandat 431.522 kb on - strandat 431.549 kb on - strandat 431.577 kb on - strandat 431.580 kb on - strandat 431.596 kb on - strandat 431.606 kb on - strandat 431.628 kb on - strandat 431.670 kb on + strandat 431.671 kb on - strandat 431.671 kb on - strandat 431.709 kb on - strandat 431.861 kb on + strand, within AO353_02025at 431.902 kb on + strand, within AO353_02025at 431.902 kb on + strand, within AO353_02025at 431.902 kb on + strand, within AO353_02025at 431.903 kb on - strand, within AO353_02025at 431.903 kb on - strand, within AO353_02025at 431.903 kb on - strand, within AO353_02025at 431.903 kb on - strand, within AO353_02025at 431.903 kb on - strand, within AO353_02025at 432.090 kb on + strand, within AO353_02025at 432.090 kb on + strand, within AO353_02025at 432.091 kb on - strand, within AO353_02025at 432.091 kb on - strand, within AO353_02025at 432.091 kb on - strand, within AO353_02025at 432.091 kb on - strand, within AO353_02025at 432.091 kb on - strand, within AO353_02025at 432.091 kb on - strand, within AO353_02025at 432.094 kb on + strand, within AO353_02025at 432.094 kb on + strand, within AO353_02025at 432.094 kb on + strand, within AO353_02025at 432.094 kb on + strand, within AO353_02025at 432.094 kb on + strand, within AO353_02025at 432.094 kb on + strand, within AO353_02025at 432.094 kb on + strand, within AO353_02025at 432.094 kb on + strand, within AO353_02025at 432.094 kb on + strand, within AO353_02025at 432.095 kb on - strand, within AO353_02025at 432.095 kb on - strand, within AO353_02025at 432.095 kb on - strand, within AO353_02025at 432.095 kb on - strand, within AO353_02025at 432.095 kb on - strand, within AO353_02025at 432.117 kb on + strand, within AO353_02025at 432.117 kb on + strand, within AO353_02025at 432.254 kb on - strand, within AO353_02025at 432.254 kb on - strand, within AO353_02025at 432.313 kb on + strand, within AO353_02025at 432.313 kb on + strand, within AO353_02025at 432.314 kb on - strand, within AO353_02025at 432.330 kb on - strand, within AO353_02025at 432.369 kb on + strand, within AO353_02025at 432.370 kb on - strand, within AO353_02025at 432.370 kb on - strand, within AO353_02025at 432.442 kb on + strand, within AO353_02025at 432.445 kb on + strand, within AO353_02025at 432.445 kb on + strand, within AO353_02025at 432.445 kb on + strand, within AO353_02025at 432.446 kb on - strand, within AO353_02025at 432.446 kb on - strand, within AO353_02025at 432.446 kb on - strand, within AO353_02025at 432.446 kb on - strand, within AO353_02025at 432.459 kb on + strand, within AO353_02025at 432.459 kb on + strand, within AO353_02025at 432.459 kb on + strand, within AO353_02025at 432.459 kb on + strand, within AO353_02025at 432.459 kb on + strand, within AO353_02025at 432.460 kb on - strand, within AO353_02025at 432.460 kb on - strand, within AO353_02025at 432.460 kb on - strand, within AO353_02025at 432.460 kb on - strand, within AO353_02025at 432.460 kb on - strand, within AO353_02025at 432.460 kb on - strand, within AO353_02025at 432.460 kb on - strand, within AO353_02025at 432.463 kb on + strand, within AO353_02025at 432.464 kb on - strand, within AO353_02025

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Per-strain Table

Position Strand Gene LocusTag Fraction N1B4wt with N2E3m denitrifying replicate C
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429,508 + AO353_02010 0.83 -1.6
429,508 + AO353_02010 0.83 +1.4
429,508 + AO353_02010 0.83 -2.3
429,508 + AO353_02010 0.83 +0.4
429,510 + AO353_02010 0.83 +0.4
429,537 - AO353_02010 0.85 -1.3
429,590 + AO353_02010 0.90 -1.8
429,631 - -0.6
429,642 - -1.3
429,642 - -1.2
429,642 - -1.6
429,642 - -2.2
429,642 - +0.3
429,715 + +1.0
429,767 - AO353_02015 0.10 -2.5
429,771 + AO353_02015 0.11 -0.8
429,771 + AO353_02015 0.11 -1.8
429,772 - AO353_02015 0.11 +1.3
429,887 + AO353_02015 0.29 -0.0
429,887 + AO353_02015 0.29 +1.0
429,888 - AO353_02015 0.29 +2.0
429,888 - AO353_02015 0.29 -2.0
429,893 + AO353_02015 0.30 +1.3
429,893 + AO353_02015 0.30 +1.0
429,894 - AO353_02015 0.30 -2.2
429,894 - AO353_02015 0.30 -2.2
429,982 + AO353_02015 0.44 -1.8
429,982 + AO353_02015 0.44 -1.8
430,005 - AO353_02015 0.48 -1.3
430,042 + AO353_02015 0.54 -0.0
430,068 + AO353_02015 0.58 -0.6
430,069 - AO353_02015 0.58 -0.0
430,069 - AO353_02015 0.58 -0.1
430,070 + AO353_02015 0.58 +1.0
430,070 + AO353_02015 0.58 +1.6
430,070 + AO353_02015 0.58 -0.6
430,070 + AO353_02015 0.58 -2.6
430,070 + AO353_02015 0.58 -0.0
430,071 - AO353_02015 0.58 -1.5
430,071 - AO353_02015 0.58 +1.0
430,071 - AO353_02015 0.58 +0.2
430,071 - AO353_02015 0.58 -1.6
430,071 - AO353_02015 0.58 -0.3
430,071 - AO353_02015 0.58 -1.0
430,072 + AO353_02015 0.58 -1.3
430,073 - AO353_02015 0.59 -0.0
430,073 - AO353_02015 0.59 -1.3
430,082 - AO353_02015 0.60 -0.0
430,094 - AO353_02015 0.62 -2.0
430,159 + AO353_02015 0.72 +0.2
430,254 + AO353_02015 0.87 -0.3
430,275 + -1.6
430,275 + -0.6
430,275 + +1.0
430,275 + -2.0
430,276 - -0.3
430,276 - +1.0
430,276 - -1.0
430,276 - -2.5
430,278 + -0.0
430,278 + -0.6
430,278 + +1.0
430,279 - -0.0
430,324 + -2.5
430,324 + -0.0
430,325 - -0.9
430,372 + -2.3
430,373 - -1.6
430,373 - -0.2
430,396 + -1.3
430,396 + -0.6
430,396 + -1.8
430,420 + -0.6
430,450 + -1.0
430,450 + -2.0
430,464 + +0.2
430,464 + +0.7
430,464 + -1.3
430,464 + +0.5
430,464 + -0.3
430,465 - -0.6
430,465 - +0.2
430,505 + +3.8
430,505 + -1.0
430,505 + +0.5
430,516 + -1.6
430,632 + AO353_02020 0.13 +1.0
430,632 + AO353_02020 0.13 -1.8
430,702 - AO353_02020 0.20 -0.6
430,748 - AO353_02020 0.25 -0.0
430,786 - AO353_02020 0.29 +1.0
430,862 + AO353_02020 0.37 -2.5
430,862 + AO353_02020 0.37 -1.3
430,863 - AO353_02020 0.37 -0.0
430,951 - AO353_02020 0.46 -1.8
431,064 - AO353_02020 0.58 +1.0
431,064 - AO353_02020 0.58 -0.6
431,133 + AO353_02020 0.65 -1.0
431,159 + AO353_02020 0.68 -0.0
431,159 + AO353_02020 0.68 -0.6
431,189 - AO353_02020 0.71 -0.6
431,189 - AO353_02020 0.71 +0.4
431,189 - AO353_02020 0.71 -1.3
431,231 + AO353_02020 0.75 -0.0
431,231 + AO353_02020 0.75 -1.0
431,232 - AO353_02020 0.75 -1.2
431,232 - AO353_02020 0.75 -1.8
431,375 + -1.0
431,407 - -2.3
431,452 - -1.6
431,452 - -2.0
431,455 + -2.0
431,456 - -0.6
431,495 + -1.0
431,522 - -0.7
431,549 - -1.2
431,577 - +2.0
431,580 - +2.3
431,596 - -1.3
431,606 - -0.0
431,628 - -1.8
431,670 + +0.4
431,671 - +1.7
431,671 - +0.8
431,709 - +0.1
431,861 + AO353_02025 0.20 -1.0
431,902 + AO353_02025 0.23 -0.8
431,902 + AO353_02025 0.23 -1.0
431,902 + AO353_02025 0.23 -0.0
431,903 - AO353_02025 0.23 +1.4
431,903 - AO353_02025 0.23 -0.0
431,903 - AO353_02025 0.23 -0.6
431,903 - AO353_02025 0.23 -1.3
431,903 - AO353_02025 0.23 +0.8
432,090 + AO353_02025 0.36 -0.2
432,090 + AO353_02025 0.36 -0.0
432,091 - AO353_02025 0.36 -2.5
432,091 - AO353_02025 0.36 -0.0
432,091 - AO353_02025 0.36 -1.0
432,091 - AO353_02025 0.36 -1.0
432,091 - AO353_02025 0.36 +1.0
432,091 - AO353_02025 0.36 -2.2
432,094 + AO353_02025 0.36 -1.0
432,094 + AO353_02025 0.36 +1.0
432,094 + AO353_02025 0.36 -0.0
432,094 + AO353_02025 0.36 +2.3
432,094 + AO353_02025 0.36 -2.2
432,094 + AO353_02025 0.36 +0.6
432,094 + AO353_02025 0.36 -2.2
432,094 + AO353_02025 0.36 -1.8
432,094 + AO353_02025 0.36 +1.0
432,095 - AO353_02025 0.36 -1.0
432,095 - AO353_02025 0.36 -1.3
432,095 - AO353_02025 0.36 +0.6
432,095 - AO353_02025 0.36 -0.2
432,095 - AO353_02025 0.36 -0.0
432,117 + AO353_02025 0.38 -0.3
432,117 + AO353_02025 0.38 +0.2
432,254 - AO353_02025 0.47 -0.6
432,254 - AO353_02025 0.47 +0.5
432,313 + AO353_02025 0.51 +2.3
432,313 + AO353_02025 0.51 -2.0
432,314 - AO353_02025 0.51 -2.5
432,330 - AO353_02025 0.52 +0.7
432,369 + AO353_02025 0.55 +2.4
432,370 - AO353_02025 0.55 -2.0
432,370 - AO353_02025 0.55 -0.3
432,442 + AO353_02025 0.60 -0.6
432,445 + AO353_02025 0.60 +0.4
432,445 + AO353_02025 0.60 -0.2
432,445 + AO353_02025 0.60 +2.0
432,446 - AO353_02025 0.60 -0.2
432,446 - AO353_02025 0.60 -0.0
432,446 - AO353_02025 0.60 -0.6
432,446 - AO353_02025 0.60 -1.0
432,459 + AO353_02025 0.61 -0.0
432,459 + AO353_02025 0.61 -1.3
432,459 + AO353_02025 0.61 +1.0
432,459 + AO353_02025 0.61 -2.2
432,459 + AO353_02025 0.61 -1.8
432,460 - AO353_02025 0.61 +1.6
432,460 - AO353_02025 0.61 -0.6
432,460 - AO353_02025 0.61 +2.0
432,460 - AO353_02025 0.61 -0.6
432,460 - AO353_02025 0.61 -1.3
432,460 - AO353_02025 0.61 -1.0
432,460 - AO353_02025 0.61 +1.0
432,463 + AO353_02025 0.61 -1.0
432,464 - AO353_02025 0.61 -0.8

Or see this region's nucleotide sequence