Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_00890

Experiment: N1B4wt with N2E3m denitrifying replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_00885 and AO353_00890 are separated by 54 nucleotidesAO353_00890 and AO353_00895 are separated by 200 nucleotides AO353_00885: AO353_00885 - 3-methylitaconate isomerase, at 200,114 to 201,304 _00885 AO353_00890: AO353_00890 - Fe/S-dependent 2-methylisocitrate dehydratase AcnD, at 201,359 to 203,953 _00890 AO353_00895: AO353_00895 - 2-methylcitrate synthase, at 204,154 to 205,281 _00895 Position (kb) 201 202 203 204Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 200.394 kb on - strand, within AO353_00885at 200.394 kb on - strand, within AO353_00885at 200.394 kb on - strand, within AO353_00885at 200.443 kb on - strandat 200.538 kb on + strand, within AO353_00885at 200.539 kb on - strand, within AO353_00885at 200.921 kb on - strand, within AO353_00885at 200.999 kb on - strand, within AO353_00885at 200.999 kb on - strand, within AO353_00885at 201.125 kb on - strandat 201.265 kb on + strandat 201.267 kb on + strandat 201.267 kb on + strandat 201.267 kb on + strandat 201.267 kb on + strandat 201.268 kb on - strandat 201.268 kb on - strandat 201.268 kb on - strandat 201.268 kb on - strandat 201.268 kb on - strandat 201.268 kb on - strandat 201.293 kb on + strandat 201.293 kb on + strandat 201.293 kb on + strandat 201.294 kb on - strandat 201.304 kb on + strandat 201.305 kb on - strandat 201.305 kb on - strandat 201.305 kb on - strandat 201.314 kb on + strandat 201.315 kb on - strandat 201.360 kb on + strandat 201.424 kb on - strandat 201.424 kb on - strandat 201.424 kb on - strandat 201.424 kb on - strandat 201.424 kb on - strandat 201.500 kb on - strandat 201.775 kb on - strand, within AO353_00890at 201.940 kb on - strand, within AO353_00890at 201.982 kb on - strand, within AO353_00890at 201.982 kb on - strand, within AO353_00890at 201.982 kb on - strand, within AO353_00890at 201.982 kb on - strand, within AO353_00890at 202.131 kb on + strand, within AO353_00890at 202.131 kb on + strand, within AO353_00890at 202.131 kb on + strand, within AO353_00890at 202.132 kb on - strand, within AO353_00890at 202.156 kb on - strand, within AO353_00890at 202.156 kb on - strand, within AO353_00890at 202.209 kb on + strand, within AO353_00890at 202.210 kb on - strand, within AO353_00890at 202.210 kb on - strand, within AO353_00890at 202.210 kb on - strand, within AO353_00890at 202.468 kb on - strand, within AO353_00890at 202.605 kb on + strand, within AO353_00890at 202.676 kb on - strand, within AO353_00890at 202.676 kb on - strand, within AO353_00890at 202.915 kb on - strand, within AO353_00890at 202.915 kb on - strand, within AO353_00890at 202.915 kb on - strand, within AO353_00890at 202.963 kb on - strand, within AO353_00890at 203.037 kb on + strand, within AO353_00890at 203.038 kb on - strand, within AO353_00890at 203.848 kb on - strandat 203.863 kb on - strandat 203.977 kb on + strandat 203.978 kb on - strandat 204.021 kb on + strandat 204.021 kb on + strandat 204.022 kb on - strandat 204.107 kb on + strandat 204.107 kb on + strandat 204.108 kb on - strandat 204.108 kb on - strandat 204.108 kb on - strandat 204.108 kb on - strandat 204.200 kb on + strandat 204.200 kb on + strandat 204.201 kb on - strandat 204.341 kb on + strand, within AO353_00895at 204.341 kb on + strand, within AO353_00895at 204.341 kb on + strand, within AO353_00895at 204.341 kb on + strand, within AO353_00895at 204.342 kb on - strand, within AO353_00895at 204.342 kb on - strand, within AO353_00895at 204.342 kb on - strand, within AO353_00895at 204.342 kb on - strand, within AO353_00895at 204.342 kb on - strand, within AO353_00895at 204.342 kb on - strand, within AO353_00895at 204.491 kb on + strand, within AO353_00895at 204.491 kb on + strand, within AO353_00895at 204.491 kb on + strand, within AO353_00895at 204.492 kb on - strand, within AO353_00895at 204.492 kb on - strand, within AO353_00895at 204.731 kb on + strand, within AO353_00895at 204.875 kb on + strand, within AO353_00895at 204.875 kb on + strand, within AO353_00895at 204.876 kb on - strand, within AO353_00895

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Per-strain Table

Position Strand Gene LocusTag Fraction N1B4wt with N2E3m denitrifying replicate C
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200,394 - AO353_00885 0.24 +1.0
200,394 - AO353_00885 0.24 -0.0
200,394 - AO353_00885 0.24 +2.2
200,443 - +3.0
200,538 + AO353_00885 0.36 -1.2
200,539 - AO353_00885 0.36 -0.1
200,921 - AO353_00885 0.68 -0.4
200,999 - AO353_00885 0.74 -1.6
200,999 - AO353_00885 0.74 -2.7
201,125 - -1.4
201,265 + -0.0
201,267 + +1.0
201,267 + +1.0
201,267 + -1.0
201,267 + -0.0
201,268 - -3.3
201,268 - +1.0
201,268 - -1.0
201,268 - +1.3
201,268 - -1.7
201,268 - +1.5
201,293 + -0.0
201,293 + -1.0
201,293 + -2.7
201,294 - -0.3
201,304 + -1.6
201,305 - -1.4
201,305 - +0.7
201,305 - -1.3
201,314 + +1.4
201,315 - -1.4
201,360 + +0.2
201,424 - +0.2
201,424 - +0.8
201,424 - -1.2
201,424 - +1.0
201,424 - +0.4
201,500 - -0.3
201,775 - AO353_00890 0.16 +1.1
201,940 - AO353_00890 0.22 -1.0
201,982 - AO353_00890 0.24 +2.2
201,982 - AO353_00890 0.24 +0.5
201,982 - AO353_00890 0.24 -0.4
201,982 - AO353_00890 0.24 -1.4
202,131 + AO353_00890 0.30 -0.6
202,131 + AO353_00890 0.30 +0.1
202,131 + AO353_00890 0.30 -1.4
202,132 - AO353_00890 0.30 -0.6
202,156 - AO353_00890 0.31 +0.1
202,156 - AO353_00890 0.31 +0.4
202,209 + AO353_00890 0.33 -1.6
202,210 - AO353_00890 0.33 +1.3
202,210 - AO353_00890 0.33 -1.0
202,210 - AO353_00890 0.33 +0.5
202,468 - AO353_00890 0.43 +0.6
202,605 + AO353_00890 0.48 -1.4
202,676 - AO353_00890 0.51 +2.2
202,676 - AO353_00890 0.51 -0.0
202,915 - AO353_00890 0.60 +0.1
202,915 - AO353_00890 0.60 -0.0
202,915 - AO353_00890 0.60 -1.6
202,963 - AO353_00890 0.62 -0.5
203,037 + AO353_00890 0.65 +1.0
203,038 - AO353_00890 0.65 -2.0
203,848 - -0.0
203,863 - -0.9
203,977 + +2.5
203,978 - +1.4
204,021 + -0.6
204,021 + -1.9
204,022 - -1.0
204,107 + -2.4
204,107 + -1.0
204,108 - +1.6
204,108 - -1.4
204,108 - -2.4
204,108 - -0.5
204,200 + -0.6
204,200 + -0.8
204,201 - +1.3
204,341 + AO353_00895 0.17 -0.2
204,341 + AO353_00895 0.17 -0.2
204,341 + AO353_00895 0.17 -1.6
204,341 + AO353_00895 0.17 +0.5
204,342 - AO353_00895 0.17 +1.6
204,342 - AO353_00895 0.17 +1.7
204,342 - AO353_00895 0.17 +0.5
204,342 - AO353_00895 0.17 +1.0
204,342 - AO353_00895 0.17 +1.5
204,342 - AO353_00895 0.17 -1.6
204,491 + AO353_00895 0.30 -0.0
204,491 + AO353_00895 0.30 +1.5
204,491 + AO353_00895 0.30 -0.0
204,492 - AO353_00895 0.30 -1.0
204,492 - AO353_00895 0.30 +1.5
204,731 + AO353_00895 0.51 -1.0
204,875 + AO353_00895 0.64 -0.0
204,875 + AO353_00895 0.64 +1.5
204,876 - AO353_00895 0.64 -1.4

Or see this region's nucleotide sequence