Experiment: m.b. Polymyxin B sulfate 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3007 and HP15_3008 overlap by 4 nucleotides HP15_3008 and HP15_3009 overlap by 4 nucleotides HP15_3009 and HP15_3010 are separated by 9 nucleotides HP15_3010 and HP15_3011 overlap by 7 nucleotides
HP15_3007: HP15_3007 - carboxyl-terminal protease family protein, at 3,152,084 to 3,153,490
_3007
HP15_3008: HP15_3008 - secreted protein containing DUF610, YibQ, at 3,153,487 to 3,154,302
_3008
HP15_3009: HP15_3009 - protein containing histidine biosynthesis, HisF domain, at 3,154,299 to 3,155,072
_3009
HP15_3010: HP15_3010 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, at 3,155,082 to 3,155,819
_3010
HP15_3011: HP15_3011 - imidazole glycerol phosphate synthase subunit HisH, at 3,155,813 to 3,156,490
_3011
Position (kb)
3154
3155
3156 Strain fitness (log2 ratio)
-1
0
1 at 3153.351 kb on - strand at 3153.503 kb on - strand at 3153.505 kb on + strand at 3153.700 kb on - strand, within HP15_3008 at 3153.815 kb on - strand, within HP15_3008 at 3153.832 kb on + strand, within HP15_3008 at 3153.881 kb on - strand, within HP15_3008 at 3153.917 kb on - strand, within HP15_3008 at 3153.960 kb on - strand, within HP15_3008 at 3154.040 kb on + strand, within HP15_3008 at 3154.149 kb on + strand, within HP15_3008 at 3154.215 kb on + strand, within HP15_3008 at 3154.267 kb on - strand at 3154.320 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0004 mg/ml remove 3,153,351 - -0.8 3,153,503 - -1.7 3,153,505 + +0.1 3,153,700 - HP15_3008 0.26 +0.8 3,153,815 - HP15_3008 0.40 -0.3 3,153,832 + HP15_3008 0.42 -0.6 3,153,881 - HP15_3008 0.48 +0.7 3,153,917 - HP15_3008 0.53 -0.2 3,153,960 - HP15_3008 0.58 +0.3 3,154,040 + HP15_3008 0.68 +0.1 3,154,149 + HP15_3008 0.81 -0.1 3,154,215 + HP15_3008 0.89 +0.9 3,154,267 - +0.1 3,154,320 - -0.1
Or see this region's nucleotide sequence