Experiment: m.b. Polymyxin B sulfate 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_254 and HP15_255 are separated by 260 nucleotides HP15_255 and HP15_256 are separated by 7 nucleotides HP15_256 and HP15_257 are separated by 6 nucleotides HP15_257 and HP15_258 are separated by 83 nucleotides
HP15_254: HP15_254 - conserved hypothetical protein, at 244,162 to 244,485
_254
HP15_255: HP15_255 - conserved hypothetical protein, at 244,746 to 245,351
_255
HP15_256: HP15_256 - serine protease, at 245,359 to 247,575
_256
HP15_257: HP15_257 - transcriptional regulator, GntR family, at 247,582 to 248,259
_257
HP15_258: HP15_258 - phospholipase D/Transphosphatidylase, at 248,343 to 249,773
_258
Position (kb) 245
246
247
248 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 244.366 kb on + strand, within HP15_254 at 244.403 kb on + strand, within HP15_254 at 244.715 kb on - strand at 244.779 kb on + strand at 244.823 kb on + strand, within HP15_255 at 244.823 kb on + strand, within HP15_255 at 244.831 kb on - strand, within HP15_255 at 245.030 kb on - strand, within HP15_255 at 245.030 kb on - strand, within HP15_255 at 245.162 kb on - strand, within HP15_255 at 245.176 kb on + strand, within HP15_255 at 245.184 kb on - strand, within HP15_255 at 245.224 kb on + strand, within HP15_255 at 245.232 kb on - strand, within HP15_255 at 245.233 kb on + strand, within HP15_255 at 245.247 kb on - strand, within HP15_255 at 245.313 kb on - strand at 245.367 kb on + strand at 245.398 kb on - strand at 245.398 kb on - strand at 245.433 kb on + strand at 245.448 kb on - strand at 245.459 kb on - strand at 245.459 kb on - strand at 245.470 kb on - strand at 245.490 kb on + strand at 245.600 kb on - strand, within HP15_256 at 245.825 kb on - strand, within HP15_256 at 245.833 kb on + strand, within HP15_256 at 245.923 kb on - strand, within HP15_256 at 246.207 kb on - strand, within HP15_256 at 246.223 kb on + strand, within HP15_256 at 246.230 kb on - strand, within HP15_256 at 246.231 kb on - strand, within HP15_256 at 246.306 kb on - strand, within HP15_256 at 246.338 kb on - strand, within HP15_256 at 246.602 kb on - strand, within HP15_256 at 246.733 kb on - strand, within HP15_256 at 246.830 kb on + strand, within HP15_256 at 246.838 kb on - strand, within HP15_256 at 246.848 kb on - strand, within HP15_256 at 246.954 kb on + strand, within HP15_256 at 246.999 kb on + strand, within HP15_256 at 247.047 kb on - strand, within HP15_256 at 247.057 kb on - strand, within HP15_256 at 247.061 kb on - strand, within HP15_256 at 247.127 kb on - strand, within HP15_256 at 247.184 kb on + strand, within HP15_256 at 247.184 kb on + strand, within HP15_256 at 247.303 kb on + strand, within HP15_256 at 247.365 kb on + strand at 247.365 kb on + strand at 247.370 kb on + strand at 247.410 kb on - strand at 247.412 kb on + strand at 247.420 kb on - strand at 247.426 kb on + strand at 247.566 kb on + strand at 247.574 kb on - strand at 247.770 kb on - strand, within HP15_257 at 247.787 kb on + strand, within HP15_257 at 247.787 kb on + strand, within HP15_257 at 247.787 kb on + strand, within HP15_257 at 247.795 kb on - strand, within HP15_257 at 247.795 kb on - strand, within HP15_257 at 247.829 kb on + strand, within HP15_257 at 247.842 kb on - strand, within HP15_257 at 247.844 kb on + strand, within HP15_257 at 247.844 kb on + strand, within HP15_257 at 247.852 kb on - strand, within HP15_257 at 247.862 kb on - strand, within HP15_257 at 248.003 kb on + strand, within HP15_257 at 248.054 kb on + strand, within HP15_257 at 248.131 kb on - strand, within HP15_257 at 248.164 kb on - strand, within HP15_257 at 248.232 kb on + strand at 248.301 kb on - strand at 248.341 kb on + strand at 248.341 kb on + strand at 248.396 kb on - strand at 248.434 kb on + strand at 248.491 kb on - strand, within HP15_258 at 248.501 kb on - strand, within HP15_258 at 248.501 kb on - strand, within HP15_258 at 248.548 kb on + strand, within HP15_258
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0004 mg/ml remove 244,366 + HP15_254 0.63 -1.8 244,403 + HP15_254 0.74 -2.2 244,715 - -0.2 244,779 + +1.5 244,823 + HP15_255 0.13 +0.8 244,823 + HP15_255 0.13 +0.7 244,831 - HP15_255 0.14 +1.2 245,030 - HP15_255 0.47 +1.9 245,030 - HP15_255 0.47 +1.8 245,162 - HP15_255 0.69 +2.1 245,176 + HP15_255 0.71 +0.5 245,184 - HP15_255 0.72 +2.0 245,224 + HP15_255 0.79 +0.3 245,232 - HP15_255 0.80 +1.0 245,233 + HP15_255 0.80 +1.9 245,247 - HP15_255 0.83 +2.5 245,313 - +1.5 245,367 + +2.5 245,398 - +2.6 245,398 - +2.4 245,433 + +2.3 245,448 - +1.8 245,459 - +1.7 245,459 - +1.2 245,470 - +1.6 245,490 + +2.0 245,600 - HP15_256 0.11 +2.4 245,825 - HP15_256 0.21 +2.2 245,833 + HP15_256 0.21 +1.9 245,923 - HP15_256 0.25 +2.7 246,207 - HP15_256 0.38 +2.2 246,223 + HP15_256 0.39 +1.9 246,230 - HP15_256 0.39 +1.6 246,231 - HP15_256 0.39 +1.9 246,306 - HP15_256 0.43 +1.9 246,338 - HP15_256 0.44 +2.8 246,602 - HP15_256 0.56 +2.0 246,733 - HP15_256 0.62 +1.6 246,830 + HP15_256 0.66 +1.9 246,838 - HP15_256 0.67 +2.4 246,848 - HP15_256 0.67 +1.9 246,954 + HP15_256 0.72 +1.9 246,999 + HP15_256 0.74 +2.0 247,047 - HP15_256 0.76 +1.8 247,057 - HP15_256 0.77 +2.5 247,061 - HP15_256 0.77 +1.8 247,127 - HP15_256 0.80 +2.7 247,184 + HP15_256 0.82 +1.8 247,184 + HP15_256 0.82 +1.0 247,303 + HP15_256 0.88 +2.5 247,365 + +1.7 247,365 + +1.9 247,370 + +2.0 247,410 - +2.5 247,412 + +2.0 247,420 - +1.7 247,426 + +1.9 247,566 + +1.5 247,574 - +1.9 247,770 - HP15_257 0.28 +0.1 247,787 + HP15_257 0.30 +0.1 247,787 + HP15_257 0.30 -0.3 247,787 + HP15_257 0.30 -0.1 247,795 - HP15_257 0.31 +0.7 247,795 - HP15_257 0.31 +0.6 247,829 + HP15_257 0.36 +1.0 247,842 - HP15_257 0.38 +1.0 247,844 + HP15_257 0.39 +0.7 247,844 + HP15_257 0.39 +1.8 247,852 - HP15_257 0.40 -0.3 247,862 - HP15_257 0.41 +0.8 248,003 + HP15_257 0.62 -0.1 248,054 + HP15_257 0.70 +0.0 248,131 - HP15_257 0.81 +0.2 248,164 - HP15_257 0.86 +0.1 248,232 + -0.0 248,301 - -1.9 248,341 + -1.2 248,341 + +0.4 248,396 - -0.1 248,434 + +0.2 248,491 - HP15_258 0.10 +0.4 248,501 - HP15_258 0.11 -0.0 248,501 - HP15_258 0.11 +0.2 248,548 + HP15_258 0.14 +0.2
Or see this region's nucleotide sequence