Experiment: m.b. Polymyxin B sulfate 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_2169 and HP15_2170 are separated by 337 nucleotides HP15_2170 and HP15_2171 are separated by 51 nucleotides HP15_2171 and HP15_2172 are separated by 251 nucleotides
HP15_2169: HP15_2169 - conserved hypothetical protein, secreted, at 2,278,321 to 2,279,448
_2169
HP15_2170: HP15_2170 - ABC-type sugar transport system, periplasmic component, at 2,279,786 to 2,281,570
_2170
HP15_2171: HP15_2171 - uracil-DNA glycosylase superfamily, at 2,281,622 to 2,282,071
_2171
HP15_2172: HP15_2172 - nitrorecductase, at 2,282,323 to 2,283,063
_2172
Position (kb)
2279
2280
2281
2282 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2278.853 kb on + strand, within HP15_2169 at 2279.148 kb on + strand, within HP15_2169 at 2279.157 kb on + strand, within HP15_2169 at 2279.157 kb on + strand, within HP15_2169 at 2279.157 kb on + strand, within HP15_2169 at 2279.157 kb on + strand, within HP15_2169 at 2279.158 kb on - strand, within HP15_2169 at 2279.353 kb on + strand at 2279.356 kb on - strand at 2279.477 kb on + strand at 2279.490 kb on + strand at 2279.852 kb on + strand at 2279.997 kb on - strand, within HP15_2170 at 2280.009 kb on + strand, within HP15_2170 at 2280.038 kb on + strand, within HP15_2170 at 2280.129 kb on + strand, within HP15_2170 at 2280.168 kb on - strand, within HP15_2170 at 2280.175 kb on + strand, within HP15_2170 at 2280.175 kb on + strand, within HP15_2170 at 2280.246 kb on - strand, within HP15_2170 at 2280.302 kb on - strand, within HP15_2170 at 2280.434 kb on + strand, within HP15_2170 at 2280.465 kb on - strand, within HP15_2170 at 2280.465 kb on - strand, within HP15_2170 at 2280.661 kb on + strand, within HP15_2170 at 2280.786 kb on + strand, within HP15_2170 at 2280.786 kb on + strand, within HP15_2170 at 2280.843 kb on - strand, within HP15_2170 at 2281.193 kb on - strand, within HP15_2170 at 2281.250 kb on + strand, within HP15_2170 at 2281.366 kb on + strand, within HP15_2170 at 2281.366 kb on + strand, within HP15_2170 at 2281.374 kb on - strand, within HP15_2170 at 2281.374 kb on - strand, within HP15_2170 at 2281.379 kb on - strand, within HP15_2170 at 2281.547 kb on + strand at 2281.738 kb on - strand, within HP15_2171 at 2281.739 kb on - strand, within HP15_2171 at 2281.755 kb on + strand, within HP15_2171 at 2281.758 kb on + strand, within HP15_2171 at 2281.806 kb on + strand, within HP15_2171 at 2282.095 kb on - strand at 2282.226 kb on + strand at 2282.438 kb on - strand, within HP15_2172 at 2282.555 kb on + strand, within HP15_2172
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0004 mg/ml remove 2,278,853 + HP15_2169 0.47 +0.4 2,279,148 + HP15_2169 0.73 +0.2 2,279,157 + HP15_2169 0.74 +0.7 2,279,157 + HP15_2169 0.74 -0.7 2,279,157 + HP15_2169 0.74 +0.0 2,279,157 + HP15_2169 0.74 +0.2 2,279,158 - HP15_2169 0.74 +0.0 2,279,353 + +0.3 2,279,356 - +0.7 2,279,477 + -0.4 2,279,490 + +0.0 2,279,852 + -0.2 2,279,997 - HP15_2170 0.12 +0.8 2,280,009 + HP15_2170 0.12 +0.8 2,280,038 + HP15_2170 0.14 -1.4 2,280,129 + HP15_2170 0.19 +0.1 2,280,168 - HP15_2170 0.21 -0.3 2,280,175 + HP15_2170 0.22 -0.4 2,280,175 + HP15_2170 0.22 -0.2 2,280,246 - HP15_2170 0.26 -0.4 2,280,302 - HP15_2170 0.29 -0.4 2,280,434 + HP15_2170 0.36 +0.3 2,280,465 - HP15_2170 0.38 -1.1 2,280,465 - HP15_2170 0.38 +0.7 2,280,661 + HP15_2170 0.49 -0.1 2,280,786 + HP15_2170 0.56 +0.3 2,280,786 + HP15_2170 0.56 -3.9 2,280,843 - HP15_2170 0.59 -0.1 2,281,193 - HP15_2170 0.79 +0.4 2,281,250 + HP15_2170 0.82 -0.0 2,281,366 + HP15_2170 0.89 -0.2 2,281,366 + HP15_2170 0.89 -0.3 2,281,374 - HP15_2170 0.89 -0.4 2,281,374 - HP15_2170 0.89 -0.0 2,281,379 - HP15_2170 0.89 -0.1 2,281,547 + +0.0 2,281,738 - HP15_2171 0.26 -0.3 2,281,739 - HP15_2171 0.26 +0.2 2,281,755 + HP15_2171 0.30 +0.6 2,281,758 + HP15_2171 0.30 +0.5 2,281,806 + HP15_2171 0.41 -0.4 2,282,095 - +0.4 2,282,226 + -0.3 2,282,438 - HP15_2172 0.16 -0.3 2,282,555 + HP15_2172 0.31 +1.5
Or see this region's nucleotide sequence