Experiment: m.b. Polymyxin B sulfate 0.0004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1232 and HP15_1233 are separated by 29 nucleotides HP15_1233 and HP15_1234 are separated by 188 nucleotides HP15_1234 and HP15_1235 are separated by 13 nucleotides HP15_1235 and HP15_1236 overlap by 4 nucleotides
HP15_1232: HP15_1232 - fatty acid oxidation complex subunit alpha, at 1,281,708 to 1,283,858
_1232
HP15_1233: HP15_1233 - acetyl-CoA acetyltransferase, at 1,283,888 to 1,285,096
_1233
HP15_1234: HP15_1234 - conserved hypothetical protein, at 1,285,285 to 1,285,548
_1234
HP15_1235: HP15_1235 - conserved hypothetical protein, at 1,285,562 to 1,285,900
_1235
HP15_1236: HP15_1236 - conserved hypothetical protein, at 1,285,897 to 1,288,026
_1236
Position (kb)
1283
1284
1285
1286 Strain fitness (log2 ratio)
-1
0
1 at 1283.187 kb on - strand, within HP15_1232 at 1283.204 kb on + strand, within HP15_1232 at 1283.205 kb on + strand, within HP15_1232 at 1283.213 kb on - strand, within HP15_1232 at 1283.267 kb on - strand, within HP15_1232 at 1283.325 kb on - strand, within HP15_1232 at 1283.397 kb on - strand, within HP15_1232 at 1283.458 kb on - strand, within HP15_1232 at 1283.646 kb on - strand at 1283.743 kb on + strand at 1283.822 kb on - strand at 1283.825 kb on + strand at 1283.825 kb on + strand at 1283.846 kb on + strand at 1283.886 kb on + strand at 1283.894 kb on - strand at 1283.894 kb on - strand at 1284.034 kb on + strand, within HP15_1233 at 1284.034 kb on - strand, within HP15_1233 at 1284.121 kb on - strand, within HP15_1233 at 1284.122 kb on - strand, within HP15_1233 at 1284.214 kb on + strand, within HP15_1233 at 1284.215 kb on - strand, within HP15_1233 at 1284.222 kb on - strand, within HP15_1233 at 1284.222 kb on - strand, within HP15_1233 at 1284.224 kb on + strand, within HP15_1233 at 1284.263 kb on + strand, within HP15_1233 at 1284.270 kb on + strand, within HP15_1233 at 1284.278 kb on - strand, within HP15_1233 at 1284.278 kb on - strand, within HP15_1233 at 1284.330 kb on - strand, within HP15_1233 at 1284.438 kb on - strand, within HP15_1233 at 1284.468 kb on + strand, within HP15_1233 at 1284.508 kb on + strand, within HP15_1233 at 1284.522 kb on + strand, within HP15_1233 at 1284.616 kb on + strand, within HP15_1233 at 1284.616 kb on - strand, within HP15_1233 at 1284.634 kb on + strand, within HP15_1233 at 1284.637 kb on - strand, within HP15_1233 at 1284.642 kb on - strand, within HP15_1233 at 1284.655 kb on - strand, within HP15_1233 at 1284.736 kb on + strand, within HP15_1233 at 1284.744 kb on - strand, within HP15_1233 at 1284.830 kb on + strand, within HP15_1233 at 1284.843 kb on - strand, within HP15_1233 at 1284.926 kb on + strand, within HP15_1233 at 1284.939 kb on + strand, within HP15_1233 at 1284.954 kb on + strand, within HP15_1233 at 1284.960 kb on + strand, within HP15_1233 at 1284.981 kb on + strand at 1284.981 kb on - strand at 1285.010 kb on + strand at 1285.059 kb on - strand at 1285.059 kb on - strand at 1285.068 kb on + strand at 1285.085 kb on - strand at 1285.179 kb on - strand at 1285.179 kb on - strand at 1285.188 kb on + strand at 1285.274 kb on - strand at 1285.346 kb on - strand, within HP15_1234 at 1285.357 kb on - strand, within HP15_1234 at 1285.407 kb on - strand, within HP15_1234 at 1285.407 kb on - strand, within HP15_1234 at 1285.468 kb on + strand, within HP15_1234 at 1285.542 kb on - strand at 1285.543 kb on + strand at 1285.632 kb on + strand, within HP15_1235 at 1285.694 kb on - strand, within HP15_1235 at 1285.774 kb on - strand, within HP15_1235 at 1285.845 kb on + strand, within HP15_1235 at 1285.845 kb on + strand, within HP15_1235 at 1285.850 kb on + strand, within HP15_1235 at 1285.853 kb on - strand, within HP15_1235 at 1285.853 kb on - strand, within HP15_1235 at 1285.858 kb on - strand, within HP15_1235 at 1285.940 kb on + strand at 1285.994 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0004 mg/ml remove 1,283,187 - HP15_1232 0.69 +0.0 1,283,204 + HP15_1232 0.70 -0.8 1,283,205 + HP15_1232 0.70 +0.9 1,283,213 - HP15_1232 0.70 -0.8 1,283,267 - HP15_1232 0.72 -0.2 1,283,325 - HP15_1232 0.75 -0.3 1,283,397 - HP15_1232 0.79 -0.7 1,283,458 - HP15_1232 0.81 +0.1 1,283,646 - -0.2 1,283,743 + -0.9 1,283,822 - +0.1 1,283,825 + -0.0 1,283,825 + -1.3 1,283,846 + +0.0 1,283,886 + -0.9 1,283,894 - +0.3 1,283,894 - +0.2 1,284,034 + HP15_1233 0.12 -0.4 1,284,034 - HP15_1233 0.12 +0.3 1,284,121 - HP15_1233 0.19 +1.3 1,284,122 - HP15_1233 0.19 -0.0 1,284,214 + HP15_1233 0.27 +0.6 1,284,215 - HP15_1233 0.27 +0.8 1,284,222 - HP15_1233 0.28 +0.1 1,284,222 - HP15_1233 0.28 -0.2 1,284,224 + HP15_1233 0.28 -0.7 1,284,263 + HP15_1233 0.31 -0.3 1,284,270 + HP15_1233 0.32 +0.8 1,284,278 - HP15_1233 0.32 -0.1 1,284,278 - HP15_1233 0.32 +0.6 1,284,330 - HP15_1233 0.37 -0.2 1,284,438 - HP15_1233 0.45 -1.3 1,284,468 + HP15_1233 0.48 -0.2 1,284,508 + HP15_1233 0.51 -0.4 1,284,522 + HP15_1233 0.52 +0.8 1,284,616 + HP15_1233 0.60 +0.3 1,284,616 - HP15_1233 0.60 +0.5 1,284,634 + HP15_1233 0.62 +0.9 1,284,637 - HP15_1233 0.62 +0.3 1,284,642 - HP15_1233 0.62 +1.4 1,284,655 - HP15_1233 0.63 -0.0 1,284,736 + HP15_1233 0.70 +0.3 1,284,744 - HP15_1233 0.71 -0.1 1,284,830 + HP15_1233 0.78 -0.4 1,284,843 - HP15_1233 0.79 -0.4 1,284,926 + HP15_1233 0.86 -0.2 1,284,939 + HP15_1233 0.87 -0.4 1,284,954 + HP15_1233 0.88 +0.1 1,284,960 + HP15_1233 0.89 -0.4 1,284,981 + +0.3 1,284,981 - +0.9 1,285,010 + +0.3 1,285,059 - -0.4 1,285,059 - -0.1 1,285,068 + -0.7 1,285,085 - +0.1 1,285,179 - -0.2 1,285,179 - -0.5 1,285,188 + +0.9 1,285,274 - +0.2 1,285,346 - HP15_1234 0.23 +0.7 1,285,357 - HP15_1234 0.27 -0.6 1,285,407 - HP15_1234 0.46 -0.1 1,285,407 - HP15_1234 0.46 +0.9 1,285,468 + HP15_1234 0.69 -0.0 1,285,542 - -0.9 1,285,543 + -0.2 1,285,632 + HP15_1235 0.21 +0.5 1,285,694 - HP15_1235 0.39 +0.1 1,285,774 - HP15_1235 0.63 +1.0 1,285,845 + HP15_1235 0.83 +0.0 1,285,845 + HP15_1235 0.83 -0.6 1,285,850 + HP15_1235 0.85 +0.7 1,285,853 - HP15_1235 0.86 +1.5 1,285,853 - HP15_1235 0.86 +0.1 1,285,858 - HP15_1235 0.87 +0.6 1,285,940 + -0.4 1,285,994 + -0.1
Or see this region's nucleotide sequence