Experiment: Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19950 and IAI47_19955 are separated by 422 nucleotides
IAI47_19950: IAI47_19950 - SDR family oxidoreductase, at 217,108 to 218,217
_19950
IAI47_19955: IAI47_19955 - site-specific integrase, at 218,640 to 219,722
_19955
Position (kb) 217
218
219 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 216.109 kb on + strand at 216.109 kb on + strand at 216.110 kb on - strand at 216.110 kb on - strand at 216.120 kb on + strand at 216.120 kb on + strand at 216.120 kb on + strand at 216.120 kb on + strand at 216.121 kb on - strand at 216.121 kb on - strand at 216.121 kb on - strand at 216.124 kb on + strand at 216.124 kb on + strand at 216.124 kb on + strand at 216.124 kb on + strand at 216.125 kb on - strand at 216.210 kb on + strand at 216.211 kb on - strand at 216.239 kb on - strand at 216.255 kb on + strand at 216.255 kb on + strand at 216.256 kb on - strand at 216.386 kb on + strand at 216.387 kb on - strand at 216.387 kb on - strand at 216.427 kb on + strand at 216.427 kb on + strand at 216.427 kb on + strand at 216.428 kb on - strand at 216.434 kb on - strand at 216.562 kb on - strand at 216.570 kb on + strand at 216.690 kb on + strand at 216.842 kb on + strand at 216.847 kb on + strand at 216.847 kb on + strand at 216.848 kb on - strand at 216.922 kb on + strand at 216.922 kb on + strand at 216.922 kb on + strand at 216.922 kb on + strand at 216.924 kb on + strand at 216.924 kb on + strand at 216.937 kb on + strand at 216.981 kb on + strand at 217.003 kb on + strand at 217.003 kb on + strand at 217.003 kb on + strand at 217.004 kb on - strand at 217.066 kb on + strand at 217.066 kb on + strand at 217.069 kb on + strand at 217.134 kb on + strand at 217.135 kb on - strand at 217.135 kb on - strand at 217.172 kb on + strand at 217.245 kb on + strand, within IAI47_19950 at 217.271 kb on + strand, within IAI47_19950 at 217.272 kb on - strand, within IAI47_19950 at 217.308 kb on + strand, within IAI47_19950 at 217.316 kb on + strand, within IAI47_19950 at 217.362 kb on + strand, within IAI47_19950 at 217.363 kb on - strand, within IAI47_19950 at 217.381 kb on - strand, within IAI47_19950 at 217.381 kb on - strand, within IAI47_19950 at 217.383 kb on + strand, within IAI47_19950 at 217.384 kb on - strand, within IAI47_19950 at 217.384 kb on - strand, within IAI47_19950 at 217.422 kb on + strand, within IAI47_19950 at 217.594 kb on + strand, within IAI47_19950 at 217.594 kb on + strand, within IAI47_19950 at 217.594 kb on + strand, within IAI47_19950 at 217.595 kb on - strand, within IAI47_19950 at 217.595 kb on - strand, within IAI47_19950 at 217.595 kb on - strand, within IAI47_19950 at 217.605 kb on + strand, within IAI47_19950 at 217.752 kb on - strand, within IAI47_19950 at 217.782 kb on + strand, within IAI47_19950 at 217.782 kb on + strand, within IAI47_19950 at 217.782 kb on + strand, within IAI47_19950 at 217.783 kb on - strand, within IAI47_19950 at 217.783 kb on - strand, within IAI47_19950 at 217.783 kb on - strand, within IAI47_19950 at 217.783 kb on - strand, within IAI47_19950 at 217.783 kb on - strand, within IAI47_19950 at 217.805 kb on - strand, within IAI47_19950 at 217.851 kb on + strand, within IAI47_19950 at 217.852 kb on - strand, within IAI47_19950 at 217.866 kb on + strand, within IAI47_19950 at 217.866 kb on + strand, within IAI47_19950 at 217.866 kb on + strand, within IAI47_19950 at 217.923 kb on + strand, within IAI47_19950 at 217.923 kb on + strand, within IAI47_19950 at 217.924 kb on - strand, within IAI47_19950 at 217.924 kb on - strand, within IAI47_19950 at 217.924 kb on - strand, within IAI47_19950 at 217.957 kb on + strand, within IAI47_19950 at 217.974 kb on + strand, within IAI47_19950 at 217.974 kb on + strand, within IAI47_19950 at 217.975 kb on - strand, within IAI47_19950 at 218.004 kb on + strand, within IAI47_19950 at 218.004 kb on + strand, within IAI47_19950 at 218.005 kb on - strand, within IAI47_19950 at 218.060 kb on - strand, within IAI47_19950 at 218.060 kb on - strand, within IAI47_19950 at 218.088 kb on - strand, within IAI47_19950 at 218.088 kb on - strand, within IAI47_19950 at 218.162 kb on + strand at 218.165 kb on + strand at 218.165 kb on + strand at 218.166 kb on - strand at 218.166 kb on - strand at 218.166 kb on - strand at 218.166 kb on - strand at 218.166 kb on - strand at 218.207 kb on - strand at 218.216 kb on - strand at 218.222 kb on + strand at 218.239 kb on + strand at 218.275 kb on + strand at 218.275 kb on + strand at 218.283 kb on + strand at 218.334 kb on - strand at 218.364 kb on + strand at 218.364 kb on + strand at 218.366 kb on + strand at 218.367 kb on - strand at 218.367 kb on - strand at 218.392 kb on + strand at 218.392 kb on + strand at 218.392 kb on + strand at 218.392 kb on + strand at 218.392 kb on + strand at 218.392 kb on + strand at 218.392 kb on + strand at 218.393 kb on - strand at 218.393 kb on - strand at 218.393 kb on - strand at 218.393 kb on - strand at 218.393 kb on - strand at 218.412 kb on + strand at 218.412 kb on + strand at 218.413 kb on - strand at 218.444 kb on + strand at 218.489 kb on + strand at 218.546 kb on + strand at 218.546 kb on + strand at 218.546 kb on + strand at 218.547 kb on - strand at 218.547 kb on - strand at 218.547 kb on - strand at 218.547 kb on - strand at 218.549 kb on + strand at 218.549 kb on + strand at 218.550 kb on - strand at 218.550 kb on - strand at 218.568 kb on - strand at 218.568 kb on - strand at 218.579 kb on - strand at 218.579 kb on - strand at 218.599 kb on + strand at 218.600 kb on - strand at 218.600 kb on - strand at 218.603 kb on + strand at 218.603 kb on + strand at 218.603 kb on + strand at 218.604 kb on - strand at 218.604 kb on - strand at 218.604 kb on - strand at 218.604 kb on - strand at 218.604 kb on - strand at 218.604 kb on - strand at 218.604 kb on - strand at 218.609 kb on + strand at 218.655 kb on + strand at 218.655 kb on + strand at 218.655 kb on + strand at 218.655 kb on + strand at 218.655 kb on + strand at 218.655 kb on + strand at 218.655 kb on + strand at 218.656 kb on - strand at 218.656 kb on - strand at 218.656 kb on - strand at 218.656 kb on - strand at 218.656 kb on - strand at 218.656 kb on - strand at 218.656 kb on - strand at 218.671 kb on + strand at 218.671 kb on + strand at 218.671 kb on + strand at 218.671 kb on + strand at 218.671 kb on + strand at 218.672 kb on - strand at 218.672 kb on - strand at 218.692 kb on + strand at 218.693 kb on - strand at 218.693 kb on - strand at 218.782 kb on + strand, within IAI47_19955 at 218.782 kb on + strand, within IAI47_19955 at 218.782 kb on + strand, within IAI47_19955 at 218.782 kb on + strand, within IAI47_19955 at 218.782 kb on + strand, within IAI47_19955 at 218.782 kb on + strand, within IAI47_19955 at 218.782 kb on + strand, within IAI47_19955 at 218.782 kb on + strand, within IAI47_19955 at 218.782 kb on + strand, within IAI47_19955 at 218.782 kb on + strand, within IAI47_19955 at 218.783 kb on - strand, within IAI47_19955 at 218.783 kb on - strand, within IAI47_19955 at 218.783 kb on - strand, within IAI47_19955 at 218.783 kb on - strand, within IAI47_19955 at 218.783 kb on - strand, within IAI47_19955 at 218.783 kb on - strand, within IAI47_19955 at 218.786 kb on + strand, within IAI47_19955 at 218.787 kb on - strand, within IAI47_19955 at 218.879 kb on - strand, within IAI47_19955 at 218.954 kb on + strand, within IAI47_19955 at 218.988 kb on + strand, within IAI47_19955 at 218.988 kb on + strand, within IAI47_19955 at 219.118 kb on + strand, within IAI47_19955 at 219.118 kb on + strand, within IAI47_19955 at 219.185 kb on - strand, within IAI47_19955 at 219.217 kb on + strand, within IAI47_19955
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4) with 1.0 mM Glucose; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 216,109 + +1.6 216,109 + -0.4 216,110 - +0.8 216,110 - -0.2 216,120 + +0.3 216,120 + -0.5 216,120 + -0.1 216,120 + +0.5 216,121 - -1.4 216,121 - -0.1 216,121 - -0.5 216,124 + +0.3 216,124 + -2.3 216,124 + +1.3 216,124 + +0.3 216,125 - +0.1 216,210 + -0.3 216,211 - -0.5 216,239 - +1.1 216,255 + -1.2 216,255 + -0.1 216,256 - -1.2 216,386 + +0.1 216,387 - +1.3 216,387 - -1.1 216,427 + +1.0 216,427 + -0.4 216,427 + -1.7 216,428 - -1.3 216,434 - -0.0 216,562 - -0.7 216,570 + +0.1 216,690 + +2.0 216,842 + -0.7 216,847 + -0.3 216,847 + +0.6 216,848 - -0.2 216,922 + +0.3 216,922 + +1.0 216,922 + -0.8 216,922 + -0.3 216,924 + +0.7 216,924 + +0.7 216,937 + +0.7 216,981 + +1.6 217,003 + -0.2 217,003 + +0.7 217,003 + +0.1 217,004 - -0.2 217,066 + -0.7 217,066 + +1.7 217,069 + +0.3 217,134 + -0.2 217,135 - -0.9 217,135 - +0.1 217,172 + +1.8 217,245 + IAI47_19950 0.12 +0.8 217,271 + IAI47_19950 0.15 -0.7 217,272 - IAI47_19950 0.15 +0.6 217,308 + IAI47_19950 0.18 -0.4 217,316 + IAI47_19950 0.19 +0.5 217,362 + IAI47_19950 0.23 +1.3 217,363 - IAI47_19950 0.23 -0.0 217,381 - IAI47_19950 0.25 -0.7 217,381 - IAI47_19950 0.25 -2.6 217,383 + IAI47_19950 0.25 -0.4 217,384 - IAI47_19950 0.25 -0.4 217,384 - IAI47_19950 0.25 +1.0 217,422 + IAI47_19950 0.28 -0.0 217,594 + IAI47_19950 0.44 -1.4 217,594 + IAI47_19950 0.44 +1.4 217,594 + IAI47_19950 0.44 +0.1 217,595 - IAI47_19950 0.44 -0.3 217,595 - IAI47_19950 0.44 -0.0 217,595 - IAI47_19950 0.44 +0.4 217,605 + IAI47_19950 0.45 -0.5 217,752 - IAI47_19950 0.58 +2.0 217,782 + IAI47_19950 0.61 -1.4 217,782 + IAI47_19950 0.61 +1.7 217,782 + IAI47_19950 0.61 -0.5 217,783 - IAI47_19950 0.61 -2.3 217,783 - IAI47_19950 0.61 -1.1 217,783 - IAI47_19950 0.61 +0.5 217,783 - IAI47_19950 0.61 -0.6 217,783 - IAI47_19950 0.61 -0.3 217,805 - IAI47_19950 0.63 -0.2 217,851 + IAI47_19950 0.67 -0.0 217,852 - IAI47_19950 0.67 -0.1 217,866 + IAI47_19950 0.68 -0.5 217,866 + IAI47_19950 0.68 +0.1 217,866 + IAI47_19950 0.68 -0.4 217,923 + IAI47_19950 0.73 +0.6 217,923 + IAI47_19950 0.73 +0.3 217,924 - IAI47_19950 0.74 -1.5 217,924 - IAI47_19950 0.74 -0.4 217,924 - IAI47_19950 0.74 -1.7 217,957 + IAI47_19950 0.76 +0.1 217,974 + IAI47_19950 0.78 -0.7 217,974 + IAI47_19950 0.78 +0.2 217,975 - IAI47_19950 0.78 -2.0 218,004 + IAI47_19950 0.81 -0.8 218,004 + IAI47_19950 0.81 +0.0 218,005 - IAI47_19950 0.81 -0.4 218,060 - IAI47_19950 0.86 +1.6 218,060 - IAI47_19950 0.86 -0.8 218,088 - IAI47_19950 0.88 -0.2 218,088 - IAI47_19950 0.88 -0.6 218,162 + +0.1 218,165 + -0.1 218,165 + +0.4 218,166 - -2.2 218,166 - -0.8 218,166 - -1.2 218,166 - -1.4 218,166 - -0.9 218,207 - -0.8 218,216 - -0.4 218,222 + +0.7 218,239 + -1.6 218,275 + +0.1 218,275 + +1.7 218,283 + +0.7 218,334 - -0.4 218,364 + -1.5 218,364 + -1.2 218,366 + +0.5 218,367 - +0.0 218,367 - -1.1 218,392 + -1.5 218,392 + -1.5 218,392 + -2.2 218,392 + -0.3 218,392 + +0.8 218,392 + -2.8 218,392 + -1.2 218,393 - -0.7 218,393 - -2.5 218,393 - +0.4 218,393 - -0.5 218,393 - -0.2 218,412 + -0.5 218,412 + +0.0 218,413 - -1.4 218,444 + +0.5 218,489 + -0.7 218,546 + +0.3 218,546 + -0.0 218,546 + -0.2 218,547 - -1.0 218,547 - +0.1 218,547 - +1.0 218,547 - -1.5 218,549 + -0.5 218,549 + -1.5 218,550 - -0.7 218,550 - -0.3 218,568 - -0.2 218,568 - -0.3 218,579 - -0.8 218,579 - -0.4 218,599 + +1.6 218,600 - +0.5 218,600 - +1.0 218,603 + -0.3 218,603 + -2.6 218,603 + +0.2 218,604 - -0.2 218,604 - +0.8 218,604 - +0.3 218,604 - +0.5 218,604 - -1.7 218,604 - +0.6 218,604 - +0.3 218,609 + +1.4 218,655 + +0.7 218,655 + +0.1 218,655 + +0.9 218,655 + +0.8 218,655 + +0.4 218,655 + +1.7 218,655 + +1.2 218,656 - +0.8 218,656 - +0.5 218,656 - -0.1 218,656 - -0.6 218,656 - +0.7 218,656 - +0.6 218,656 - -0.3 218,671 + +1.0 218,671 + -0.3 218,671 + +0.3 218,671 + +1.0 218,671 + +0.4 218,672 - +1.2 218,672 - -0.8 218,692 + +0.5 218,693 - +1.8 218,693 - -0.5 218,782 + IAI47_19955 0.13 +2.8 218,782 + IAI47_19955 0.13 -0.0 218,782 + IAI47_19955 0.13 +0.0 218,782 + IAI47_19955 0.13 +1.7 218,782 + IAI47_19955 0.13 +0.1 218,782 + IAI47_19955 0.13 +3.4 218,782 + IAI47_19955 0.13 -0.1 218,782 + IAI47_19955 0.13 +0.6 218,782 + IAI47_19955 0.13 +1.0 218,782 + IAI47_19955 0.13 +1.0 218,783 - IAI47_19955 0.13 -1.4 218,783 - IAI47_19955 0.13 -1.1 218,783 - IAI47_19955 0.13 -2.2 218,783 - IAI47_19955 0.13 -1.6 218,783 - IAI47_19955 0.13 +0.7 218,783 - IAI47_19955 0.13 -1.0 218,786 + IAI47_19955 0.13 +0.9 218,787 - IAI47_19955 0.14 +1.0 218,879 - IAI47_19955 0.22 -1.8 218,954 + IAI47_19955 0.29 +2.8 218,988 + IAI47_19955 0.32 -0.4 218,988 + IAI47_19955 0.32 +0.3 219,118 + IAI47_19955 0.44 -1.0 219,118 + IAI47_19955 0.44 +1.4 219,185 - IAI47_19955 0.50 -0.9 219,217 + IAI47_19955 0.53 +0.2
Or see this region's nucleotide sequence