Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08410

Experiment: nitrite 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08405 and BWI76_RS08410 are separated by 15 nucleotidesBWI76_RS08410 and BWI76_RS08415 are separated by 380 nucleotides BWI76_RS08405: BWI76_RS08405 - succinate dehydrogenase flavoprotein subunit, at 1,776,346 to 1,778,112 _RS08405 BWI76_RS08410: BWI76_RS08410 - succinate dehydrogenase iron-sulfur subunit, at 1,778,128 to 1,778,844 _RS08410 BWI76_RS08415: BWI76_RS08415 - 2-oxoglutarate dehydrogenase subunit E1, at 1,779,225 to 1,782,032 _RS08415 Position (kb) 1778 1779Strain fitness (log2 ratio) -2 -1 0 1at 1777.484 kb on + strand, within BWI76_RS08405at 1777.793 kb on + strand, within BWI76_RS08405at 1778.846 kb on - strandat 1778.981 kb on + strandat 1779.007 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 20 mM
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1,777,484 + BWI76_RS08405 0.64 -1.8
1,777,793 + BWI76_RS08405 0.82 -0.0
1,778,846 - -1.4
1,778,981 + -1.3
1,779,007 + +0.3

Or see this region's nucleotide sequence