Experiment: Varel_Bryant medium with Tetrazolium violet 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0657 and BT0658 are separated by 23 nucleotides BT0658 and BT0659 are separated by 89 nucleotides BT0659 and BT0660 are separated by 188 nucleotides
BT0657: BT0657 - ATP-dependent DNA helicase (NCBI ptt file), at 808,554 to 810,920
BT0657
BT0658: BT0658 - hypothetical protein (NCBI ptt file), at 810,944 to 811,639
BT0658
BT0659: BT0659 - hypothetical protein (NCBI ptt file), at 811,729 to 812,766
BT0659
BT0660: BT0660 - conserved hypothetical protein (NCBI ptt file), at 812,955 to 814,106
BT0660
Position (kb)
811
812
813 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 810.884 kb on - strand at 810.934 kb on - strand at 810.963 kb on + strand at 811.010 kb on - strand at 811.032 kb on + strand, within BT0658 at 811.138 kb on + strand, within BT0658 at 811.212 kb on - strand, within BT0658 at 811.217 kb on + strand, within BT0658 at 811.217 kb on + strand, within BT0658 at 811.253 kb on - strand, within BT0658 at 811.421 kb on - strand, within BT0658 at 811.421 kb on - strand, within BT0658 at 811.470 kb on + strand, within BT0658 at 811.509 kb on - strand, within BT0658 at 811.517 kb on - strand, within BT0658 at 811.648 kb on + strand at 811.682 kb on - strand at 811.682 kb on - strand at 811.711 kb on - strand at 811.717 kb on - strand at 811.717 kb on - strand at 811.726 kb on - strand at 811.747 kb on + strand at 811.760 kb on + strand at 811.777 kb on + strand at 811.777 kb on + strand at 811.778 kb on - strand at 811.822 kb on + strand at 811.822 kb on + strand at 811.823 kb on - strand at 811.825 kb on - strand at 811.830 kb on + strand at 811.892 kb on - strand, within BT0659 at 811.936 kb on - strand, within BT0659 at 811.938 kb on - strand, within BT0659 at 811.938 kb on - strand, within BT0659 at 811.943 kb on - strand, within BT0659 at 811.947 kb on - strand, within BT0659 at 811.947 kb on - strand, within BT0659 at 811.947 kb on - strand, within BT0659 at 811.952 kb on + strand, within BT0659 at 811.953 kb on - strand, within BT0659 at 811.953 kb on - strand, within BT0659 at 811.953 kb on - strand, within BT0659 at 811.956 kb on + strand, within BT0659 at 812.015 kb on + strand, within BT0659 at 812.016 kb on - strand, within BT0659 at 812.052 kb on - strand, within BT0659 at 812.097 kb on + strand, within BT0659 at 812.116 kb on + strand at 812.252 kb on - strand, within BT0659 at 812.253 kb on + strand, within BT0659 at 812.253 kb on + strand, within BT0659 at 812.277 kb on + strand, within BT0659 at 812.365 kb on + strand, within BT0659 at 812.411 kb on - strand, within BT0659 at 812.412 kb on + strand, within BT0659 at 812.562 kb on - strand, within BT0659 at 812.563 kb on + strand, within BT0659 at 812.597 kb on - strand, within BT0659 at 812.597 kb on - strand, within BT0659 at 812.602 kb on - strand, within BT0659 at 812.791 kb on + strand at 812.792 kb on - strand at 812.803 kb on + strand at 812.843 kb on + strand at 813.197 kb on + strand, within BT0660 at 813.222 kb on - strand, within BT0660 at 813.230 kb on - strand, within BT0660 at 813.230 kb on - strand, within BT0660 at 813.242 kb on + strand, within BT0660 at 813.246 kb on + strand, within BT0660 at 813.272 kb on + strand, within BT0660 at 813.273 kb on - strand, within BT0660 at 813.336 kb on - strand, within BT0660 at 813.427 kb on + strand, within BT0660 at 813.428 kb on - strand, within BT0660 at 813.475 kb on + strand, within BT0660 at 813.516 kb on - strand, within BT0660 at 813.545 kb on + strand, within BT0660 at 813.556 kb on - strand, within BT0660 at 813.579 kb on + strand, within BT0660 at 813.641 kb on + strand, within BT0660 at 813.642 kb on - strand, within BT0660
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Tetrazolium violet 0.025 mM remove 810,884 - -0.0 810,934 - -0.6 810,963 + -0.3 811,010 - -0.6 811,032 + BT0658 0.13 -1.6 811,138 + BT0658 0.28 -1.6 811,212 - BT0658 0.39 +0.5 811,217 + BT0658 0.39 -1.7 811,217 + BT0658 0.39 -0.6 811,253 - BT0658 0.44 +1.0 811,421 - BT0658 0.69 -1.0 811,421 - BT0658 0.69 -2.2 811,470 + BT0658 0.76 +1.0 811,509 - BT0658 0.81 -0.6 811,517 - BT0658 0.82 -0.6 811,648 + -0.9 811,682 - -0.8 811,682 - -1.4 811,711 - +0.3 811,717 - -1.3 811,717 - -2.8 811,726 - +1.0 811,747 + -0.6 811,760 + -0.3 811,777 + -0.1 811,777 + +0.2 811,778 - -0.5 811,822 + +0.5 811,822 + +0.4 811,823 - -1.9 811,825 - -0.4 811,830 + -1.4 811,892 - BT0659 0.16 -0.6 811,936 - BT0659 0.20 -1.6 811,938 - BT0659 0.20 -0.8 811,938 - BT0659 0.20 -0.1 811,943 - BT0659 0.21 +1.9 811,947 - BT0659 0.21 -0.1 811,947 - BT0659 0.21 -0.9 811,947 - BT0659 0.21 +0.7 811,952 + BT0659 0.21 -0.1 811,953 - BT0659 0.22 -0.6 811,953 - BT0659 0.22 +1.4 811,953 - BT0659 0.22 +0.4 811,956 + BT0659 0.22 -0.3 812,015 + BT0659 0.28 +0.9 812,016 - BT0659 0.28 -1.2 812,052 - BT0659 0.31 -1.0 812,097 + BT0659 0.35 +2.3 812,116 + -0.1 812,252 - BT0659 0.50 -0.5 812,253 + BT0659 0.50 -1.2 812,253 + BT0659 0.50 -1.4 812,277 + BT0659 0.53 +0.9 812,365 + BT0659 0.61 -1.4 812,411 - BT0659 0.66 +0.2 812,412 + BT0659 0.66 -1.9 812,562 - BT0659 0.80 +1.7 812,563 + BT0659 0.80 +0.9 812,597 - BT0659 0.84 -0.9 812,597 - BT0659 0.84 +1.5 812,602 - BT0659 0.84 -0.4 812,791 + -1.1 812,792 - -0.1 812,803 + -0.1 812,843 + -1.9 813,197 + BT0660 0.21 -1.7 813,222 - BT0660 0.23 -0.0 813,230 - BT0660 0.24 -1.0 813,230 - BT0660 0.24 -0.0 813,242 + BT0660 0.25 -0.0 813,246 + BT0660 0.25 -0.0 813,272 + BT0660 0.28 -1.9 813,273 - BT0660 0.28 -0.0 813,336 - BT0660 0.33 -0.6 813,427 + BT0660 0.41 -0.3 813,428 - BT0660 0.41 -0.0 813,475 + BT0660 0.45 +2.5 813,516 - BT0660 0.49 -3.2 813,545 + BT0660 0.51 -1.4 813,556 - BT0660 0.52 -0.6 813,579 + BT0660 0.54 -1.0 813,641 + BT0660 0.60 -0.6 813,642 - BT0660 0.60 -0.2
Or see this region's nucleotide sequence