Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04200 and PGA1_c04210 overlap by 4 nucleotides PGA1_c04210 and PGA1_c04220 are separated by 48 nucleotides PGA1_c04220 and PGA1_c04230 are separated by 43 nucleotides
PGA1_c04200: PGA1_c04200 - phosphate uptake ABC transporter permease protein PhoE, at 407,650 to 408,519
_c04200
PGA1_c04210: PGA1_c04210 - phosphate uptake ABC transporter permease protein PhoT, at 408,516 to 409,886
_c04210
PGA1_c04220: PGA1_c04220 - putative phosphonate metabolism, at 409,935 to 410,549
_c04220
PGA1_c04230: PGA1_c04230 - putative phosphonates metabolism transcriptional regulator phnF, at 410,593 to 411,327
_c04230
Position (kb) 408
409
410 Strain fitness (log2 ratio) -2
-1
0
1 at 407.517 kb on + strand at 407.520 kb on + strand at 407.528 kb on - strand at 407.537 kb on + strand at 407.569 kb on - strand at 407.571 kb on + strand at 407.571 kb on + strand at 407.571 kb on + strand at 407.571 kb on + strand at 407.571 kb on + strand at 407.571 kb on + strand at 407.571 kb on + strand at 407.579 kb on - strand at 407.579 kb on - strand at 407.579 kb on - strand at 407.579 kb on - strand at 407.579 kb on - strand at 407.579 kb on - strand at 407.579 kb on - strand at 407.579 kb on - strand at 407.579 kb on - strand at 407.579 kb on - strand at 407.582 kb on - strand at 407.665 kb on + strand at 407.737 kb on - strand, within PGA1_c04200 at 407.806 kb on + strand, within PGA1_c04200 at 407.859 kb on + strand, within PGA1_c04200 at 407.885 kb on - strand, within PGA1_c04200 at 407.902 kb on + strand, within PGA1_c04200 at 407.902 kb on + strand, within PGA1_c04200 at 407.907 kb on + strand, within PGA1_c04200 at 407.980 kb on + strand, within PGA1_c04200 at 407.980 kb on + strand, within PGA1_c04200 at 408.012 kb on - strand, within PGA1_c04200 at 408.124 kb on - strand, within PGA1_c04200 at 408.165 kb on + strand, within PGA1_c04200 at 408.225 kb on - strand, within PGA1_c04200 at 408.255 kb on + strand, within PGA1_c04200 at 408.256 kb on + strand, within PGA1_c04200 at 408.280 kb on + strand, within PGA1_c04200 at 408.304 kb on + strand, within PGA1_c04200 at 408.379 kb on - strand, within PGA1_c04200 at 408.395 kb on + strand, within PGA1_c04200 at 408.486 kb on - strand at 408.539 kb on + strand at 408.541 kb on + strand at 408.541 kb on + strand at 408.549 kb on - strand at 408.549 kb on - strand at 408.549 kb on - strand at 408.549 kb on - strand at 408.549 kb on - strand at 408.549 kb on - strand at 408.611 kb on - strand at 408.793 kb on - strand, within PGA1_c04210 at 408.897 kb on + strand, within PGA1_c04210 at 408.945 kb on + strand, within PGA1_c04210 at 409.062 kb on + strand, within PGA1_c04210 at 409.127 kb on - strand, within PGA1_c04210 at 409.127 kb on - strand, within PGA1_c04210 at 409.132 kb on - strand, within PGA1_c04210 at 409.137 kb on - strand, within PGA1_c04210 at 409.153 kb on - strand, within PGA1_c04210 at 409.215 kb on + strand, within PGA1_c04210 at 409.228 kb on - strand, within PGA1_c04210 at 409.231 kb on + strand, within PGA1_c04210 at 409.244 kb on - strand, within PGA1_c04210 at 409.296 kb on + strand, within PGA1_c04210 at 409.316 kb on + strand, within PGA1_c04210 at 409.354 kb on + strand, within PGA1_c04210 at 409.377 kb on - strand, within PGA1_c04210 at 409.396 kb on - strand, within PGA1_c04210 at 409.417 kb on - strand, within PGA1_c04210 at 409.417 kb on - strand, within PGA1_c04210 at 409.417 kb on - strand, within PGA1_c04210 at 409.422 kb on - strand, within PGA1_c04210 at 409.441 kb on + strand, within PGA1_c04210 at 409.494 kb on - strand, within PGA1_c04210 at 409.570 kb on + strand, within PGA1_c04210 at 409.573 kb on - strand, within PGA1_c04210 at 409.578 kb on - strand, within PGA1_c04210 at 409.588 kb on + strand, within PGA1_c04210 at 409.594 kb on + strand, within PGA1_c04210 at 409.596 kb on - strand, within PGA1_c04210 at 409.596 kb on - strand, within PGA1_c04210 at 409.596 kb on - strand, within PGA1_c04210 at 409.612 kb on + strand, within PGA1_c04210 at 409.681 kb on - strand, within PGA1_c04210 at 409.718 kb on + strand, within PGA1_c04210 at 409.839 kb on - strand at 409.877 kb on - strand at 409.880 kb on + strand at 409.888 kb on - strand at 409.910 kb on + strand at 409.918 kb on - strand at 409.918 kb on - strand at 409.923 kb on - strand at 409.943 kb on + strand at 409.970 kb on + strand at 410.027 kb on - strand, within PGA1_c04220 at 410.027 kb on - strand, within PGA1_c04220 at 410.029 kb on + strand, within PGA1_c04220 at 410.037 kb on - strand, within PGA1_c04220 at 410.108 kb on + strand, within PGA1_c04220 at 410.128 kb on - strand, within PGA1_c04220 at 410.133 kb on - strand, within PGA1_c04220 at 410.159 kb on + strand, within PGA1_c04220 at 410.212 kb on + strand, within PGA1_c04220 at 410.246 kb on + strand, within PGA1_c04220 at 410.254 kb on - strand, within PGA1_c04220 at 410.254 kb on - strand, within PGA1_c04220 at 410.305 kb on + strand, within PGA1_c04220 at 410.305 kb on + strand, within PGA1_c04220 at 410.305 kb on + strand, within PGA1_c04220 at 410.308 kb on - strand, within PGA1_c04220 at 410.317 kb on + strand, within PGA1_c04220 at 410.323 kb on + strand, within PGA1_c04220 at 410.329 kb on + strand, within PGA1_c04220 at 410.385 kb on + strand, within PGA1_c04220 at 410.419 kb on + strand, within PGA1_c04220 at 410.483 kb on + strand, within PGA1_c04220 at 410.491 kb on - strand at 410.496 kb on + strand at 410.518 kb on + strand at 410.549 kb on + strand at 410.557 kb on - strand at 410.559 kb on + strand at 410.572 kb on - strand at 410.631 kb on + strand at 410.639 kb on - strand at 410.639 kb on - strand at 410.639 kb on - strand at 410.657 kb on + strand at 410.706 kb on + strand, within PGA1_c04230 at 410.707 kb on - strand, within PGA1_c04230 at 410.709 kb on - strand, within PGA1_c04230 at 410.768 kb on + strand, within PGA1_c04230 at 410.768 kb on - strand, within PGA1_c04230 at 410.832 kb on - strand, within PGA1_c04230
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 407,517 + -1.2 407,520 + -0.7 407,528 - +0.4 407,537 + -0.1 407,569 - -0.8 407,571 + +0.2 407,571 + -0.1 407,571 + -0.3 407,571 + +0.6 407,571 + +0.7 407,571 + -0.3 407,571 + -0.1 407,579 - -1.8 407,579 - -0.2 407,579 - +0.3 407,579 - +0.2 407,579 - -0.7 407,579 - +0.0 407,579 - +0.4 407,579 - +0.4 407,579 - -0.8 407,579 - -0.1 407,582 - -1.5 407,665 + -1.3 407,737 - PGA1_c04200 0.10 -0.3 407,806 + PGA1_c04200 0.18 +0.3 407,859 + PGA1_c04200 0.24 -1.8 407,885 - PGA1_c04200 0.27 -1.7 407,902 + PGA1_c04200 0.29 -0.9 407,902 + PGA1_c04200 0.29 -1.7 407,907 + PGA1_c04200 0.30 -2.0 407,980 + PGA1_c04200 0.38 -0.5 407,980 + PGA1_c04200 0.38 +0.9 408,012 - PGA1_c04200 0.42 -0.5 408,124 - PGA1_c04200 0.54 +0.1 408,165 + PGA1_c04200 0.59 -0.1 408,225 - PGA1_c04200 0.66 +0.6 408,255 + PGA1_c04200 0.70 -0.1 408,256 + PGA1_c04200 0.70 +0.2 408,280 + PGA1_c04200 0.72 +1.6 408,304 + PGA1_c04200 0.75 +0.0 408,379 - PGA1_c04200 0.84 -0.3 408,395 + PGA1_c04200 0.86 -1.1 408,486 - +1.7 408,539 + +0.4 408,541 + -1.3 408,541 + -0.1 408,549 - -0.6 408,549 - -1.5 408,549 - -2.3 408,549 - -0.1 408,549 - -0.7 408,549 - -0.6 408,611 - -0.1 408,793 - PGA1_c04210 0.20 +0.1 408,897 + PGA1_c04210 0.28 -0.4 408,945 + PGA1_c04210 0.31 -0.4 409,062 + PGA1_c04210 0.40 -0.1 409,127 - PGA1_c04210 0.45 +0.9 409,127 - PGA1_c04210 0.45 -0.7 409,132 - PGA1_c04210 0.45 -1.0 409,137 - PGA1_c04210 0.45 +0.3 409,153 - PGA1_c04210 0.46 -1.6 409,215 + PGA1_c04210 0.51 -0.7 409,228 - PGA1_c04210 0.52 +0.0 409,231 + PGA1_c04210 0.52 -0.5 409,244 - PGA1_c04210 0.53 +0.3 409,296 + PGA1_c04210 0.57 -0.7 409,316 + PGA1_c04210 0.58 -0.8 409,354 + PGA1_c04210 0.61 +0.1 409,377 - PGA1_c04210 0.63 +0.6 409,396 - PGA1_c04210 0.64 +0.2 409,417 - PGA1_c04210 0.66 +0.3 409,417 - PGA1_c04210 0.66 -0.1 409,417 - PGA1_c04210 0.66 -0.5 409,422 - PGA1_c04210 0.66 -1.1 409,441 + PGA1_c04210 0.67 -0.6 409,494 - PGA1_c04210 0.71 -1.4 409,570 + PGA1_c04210 0.77 -0.7 409,573 - PGA1_c04210 0.77 -0.7 409,578 - PGA1_c04210 0.77 +1.0 409,588 + PGA1_c04210 0.78 -0.4 409,594 + PGA1_c04210 0.79 -0.1 409,596 - PGA1_c04210 0.79 -0.9 409,596 - PGA1_c04210 0.79 +0.1 409,596 - PGA1_c04210 0.79 +0.3 409,612 + PGA1_c04210 0.80 -1.0 409,681 - PGA1_c04210 0.85 -0.5 409,718 + PGA1_c04210 0.88 +1.1 409,839 - +0.3 409,877 - -0.4 409,880 + +0.6 409,888 - +0.7 409,910 + +0.4 409,918 - -0.5 409,918 - +0.2 409,923 - +0.5 409,943 + -2.3 409,970 + +0.7 410,027 - PGA1_c04220 0.15 -1.2 410,027 - PGA1_c04220 0.15 -0.2 410,029 + PGA1_c04220 0.15 -0.6 410,037 - PGA1_c04220 0.17 -0.8 410,108 + PGA1_c04220 0.28 +0.6 410,128 - PGA1_c04220 0.31 -1.2 410,133 - PGA1_c04220 0.32 -0.3 410,159 + PGA1_c04220 0.36 -0.3 410,212 + PGA1_c04220 0.45 +0.9 410,246 + PGA1_c04220 0.51 -0.6 410,254 - PGA1_c04220 0.52 -0.5 410,254 - PGA1_c04220 0.52 +1.7 410,305 + PGA1_c04220 0.60 -1.4 410,305 + PGA1_c04220 0.60 -0.4 410,305 + PGA1_c04220 0.60 -0.5 410,308 - PGA1_c04220 0.61 -0.4 410,317 + PGA1_c04220 0.62 -0.3 410,323 + PGA1_c04220 0.63 -0.0 410,329 + PGA1_c04220 0.64 +0.8 410,385 + PGA1_c04220 0.73 +1.1 410,419 + PGA1_c04220 0.79 -0.1 410,483 + PGA1_c04220 0.89 +0.7 410,491 - +0.4 410,496 + +0.5 410,518 + +0.7 410,549 + +0.1 410,557 - +0.9 410,559 + +0.7 410,572 - +0.4 410,631 + +0.7 410,639 - -1.0 410,639 - -1.6 410,639 - +0.4 410,657 + -1.6 410,706 + PGA1_c04230 0.15 +1.0 410,707 - PGA1_c04230 0.16 +0.4 410,709 - PGA1_c04230 0.16 -0.3 410,768 + PGA1_c04230 0.24 -1.9 410,768 - PGA1_c04230 0.24 -0.6 410,832 - PGA1_c04230 0.33 -0.1
Or see this region's nucleotide sequence