Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_65p00140 and PGA1_65p00150 are separated by 181 nucleotides PGA1_65p00150 and PGA1_65p00160 overlap by 4 nucleotides PGA1_65p00160 and PGA1_65p00170 are separated by 388 nucleotides
PGA1_65p00140: PGA1_65p00140 - hypothetical protein, at 18,661 to 18,867
_65p00140
PGA1_65p00150: PGA1_65p00150 - Protein of unknown function (DUF2886)., at 19,049 to 19,774
_65p00150
PGA1_65p00160: PGA1_65p00160 - Bacterial putative lipoprotein (DUF940)., at 19,771 to 21,903
_65p00160
PGA1_65p00170: PGA1_65p00170 - putative capsular polysaccharide biosynthesis protein, at 22,292 to 24,139
_65p00170
Position (kb) 19
20
21
22 Strain fitness (log2 ratio) -1
0
1
2 at 18.835 kb on + strand, within PGA1_65p00140 at 18.879 kb on + strand at 18.901 kb on + strand at 18.901 kb on + strand at 18.904 kb on - strand at 19.018 kb on - strand at 19.218 kb on + strand, within PGA1_65p00150 at 19.303 kb on - strand, within PGA1_65p00150 at 19.369 kb on + strand, within PGA1_65p00150 at 19.369 kb on + strand, within PGA1_65p00150 at 19.377 kb on - strand, within PGA1_65p00150 at 19.407 kb on - strand at 19.559 kb on - strand, within PGA1_65p00150 at 19.570 kb on + strand, within PGA1_65p00150 at 19.609 kb on + strand, within PGA1_65p00150 at 19.620 kb on + strand, within PGA1_65p00150 at 19.638 kb on - strand, within PGA1_65p00150 at 19.811 kb on - strand at 19.962 kb on + strand at 19.970 kb on - strand at 20.114 kb on - strand, within PGA1_65p00160 at 20.306 kb on - strand, within PGA1_65p00160 at 20.372 kb on + strand, within PGA1_65p00160 at 20.515 kb on + strand, within PGA1_65p00160 at 20.576 kb on + strand, within PGA1_65p00160 at 20.682 kb on + strand, within PGA1_65p00160 at 20.842 kb on + strand, within PGA1_65p00160 at 21.151 kb on + strand, within PGA1_65p00160 at 21.174 kb on - strand, within PGA1_65p00160 at 21.213 kb on + strand, within PGA1_65p00160 at 21.609 kb on + strand at 21.696 kb on + strand at 21.839 kb on - strand at 21.999 kb on + strand at 22.136 kb on + strand at 22.220 kb on + strand at 22.386 kb on + strand at 22.394 kb on - strand at 22.480 kb on + strand, within PGA1_65p00170 at 22.480 kb on + strand, within PGA1_65p00170 at 22.691 kb on - strand, within PGA1_65p00170
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 18,835 + PGA1_65p00140 0.84 -0.3 18,879 + -0.9 18,901 + +0.1 18,901 + +1.2 18,904 - -1.5 19,018 - +0.8 19,218 + PGA1_65p00150 0.23 +0.5 19,303 - PGA1_65p00150 0.35 -0.4 19,369 + PGA1_65p00150 0.44 +0.1 19,369 + PGA1_65p00150 0.44 -0.2 19,377 - PGA1_65p00150 0.45 +0.5 19,407 - -0.1 19,559 - PGA1_65p00150 0.70 -0.9 19,570 + PGA1_65p00150 0.72 +0.6 19,609 + PGA1_65p00150 0.77 +0.0 19,620 + PGA1_65p00150 0.79 +0.8 19,638 - PGA1_65p00150 0.81 +0.6 19,811 - +1.0 19,962 + +0.2 19,970 - -1.1 20,114 - PGA1_65p00160 0.16 -0.1 20,306 - PGA1_65p00160 0.25 +0.7 20,372 + PGA1_65p00160 0.28 +0.9 20,515 + PGA1_65p00160 0.35 +1.0 20,576 + PGA1_65p00160 0.38 +1.1 20,682 + PGA1_65p00160 0.43 +0.3 20,842 + PGA1_65p00160 0.50 -0.9 21,151 + PGA1_65p00160 0.65 -0.1 21,174 - PGA1_65p00160 0.66 +0.5 21,213 + PGA1_65p00160 0.68 -0.7 21,609 + +0.1 21,696 + +1.0 21,839 - +0.3 21,999 + +2.0 22,136 + +1.0 22,220 + -1.6 22,386 + -1.1 22,394 - +1.1 22,480 + PGA1_65p00170 0.10 -0.5 22,480 + PGA1_65p00170 0.10 -0.6 22,691 - PGA1_65p00170 0.22 -0.1
Or see this region's nucleotide sequence