Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c35610 and PGA1_c35620 overlap by 4 nucleotides PGA1_c35620 and PGA1_c35630 overlap by 8 nucleotides PGA1_c35630 and PGA1_c35640 overlap by 1 nucleotides
PGA1_c35610: PGA1_c35610 - flagellar biosynthetic protein FliP, at 3,734,566 to 3,735,309
_c35610
PGA1_c35620: PGA1_c35620 - flagellar motor switch protein FliN, at 3,735,306 to 3,735,602
_c35620
PGA1_c35630: PGA1_c35630 - putative flagellar biosynthesis protein, at 3,735,595 to 3,736,191
_c35630
PGA1_c35640: PGA1_c35640 - flagellar M-ring protein FliF, at 3,736,191 to 3,737,876
_c35640
Position (kb)
3735
3736 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3734.366 kb on + strand at 3734.388 kb on + strand at 3734.420 kb on + strand at 3734.420 kb on + strand at 3734.438 kb on - strand at 3734.438 kb on - strand at 3734.455 kb on + strand at 3734.463 kb on - strand at 3734.463 kb on - strand at 3734.515 kb on + strand at 3734.517 kb on + strand at 3734.518 kb on - strand at 3734.604 kb on + strand at 3734.604 kb on + strand at 3734.604 kb on + strand at 3734.612 kb on - strand at 3734.612 kb on - strand at 3734.612 kb on - strand at 3734.681 kb on - strand, within PGA1_c35610 at 3734.694 kb on + strand, within PGA1_c35610 at 3734.728 kb on - strand, within PGA1_c35610 at 3734.751 kb on + strand, within PGA1_c35610 at 3734.759 kb on - strand, within PGA1_c35610 at 3734.778 kb on + strand, within PGA1_c35610 at 3734.793 kb on - strand, within PGA1_c35610 at 3734.809 kb on + strand, within PGA1_c35610 at 3734.831 kb on - strand, within PGA1_c35610 at 3734.832 kb on + strand, within PGA1_c35610 at 3734.837 kb on + strand, within PGA1_c35610 at 3734.837 kb on + strand, within PGA1_c35610 at 3734.876 kb on + strand, within PGA1_c35610 at 3734.899 kb on - strand, within PGA1_c35610 at 3734.899 kb on - strand, within PGA1_c35610 at 3734.899 kb on - strand, within PGA1_c35610 at 3734.899 kb on - strand, within PGA1_c35610 at 3734.909 kb on - strand, within PGA1_c35610 at 3734.977 kb on + strand, within PGA1_c35610 at 3734.986 kb on - strand, within PGA1_c35610 at 3735.027 kb on + strand, within PGA1_c35610 at 3735.027 kb on + strand, within PGA1_c35610 at 3735.027 kb on + strand, within PGA1_c35610 at 3735.027 kb on + strand, within PGA1_c35610 at 3735.035 kb on - strand, within PGA1_c35610 at 3735.035 kb on - strand, within PGA1_c35610 at 3735.035 kb on - strand, within PGA1_c35610 at 3735.035 kb on - strand, within PGA1_c35610 at 3735.035 kb on - strand, within PGA1_c35610 at 3735.041 kb on + strand, within PGA1_c35610 at 3735.071 kb on + strand, within PGA1_c35610 at 3735.071 kb on + strand, within PGA1_c35610 at 3735.138 kb on + strand, within PGA1_c35610 at 3735.138 kb on + strand, within PGA1_c35610 at 3735.146 kb on - strand, within PGA1_c35610 at 3735.146 kb on - strand, within PGA1_c35610 at 3735.152 kb on - strand, within PGA1_c35610 at 3735.185 kb on - strand, within PGA1_c35610 at 3735.194 kb on + strand, within PGA1_c35610 at 3735.194 kb on + strand, within PGA1_c35610 at 3735.202 kb on - strand, within PGA1_c35610 at 3735.216 kb on + strand, within PGA1_c35610 at 3735.303 kb on + strand at 3735.344 kb on + strand, within PGA1_c35620 at 3735.352 kb on - strand, within PGA1_c35620 at 3735.352 kb on - strand, within PGA1_c35620 at 3735.354 kb on + strand, within PGA1_c35620 at 3735.395 kb on - strand, within PGA1_c35620 at 3735.411 kb on + strand, within PGA1_c35620 at 3735.421 kb on - strand, within PGA1_c35620 at 3735.479 kb on + strand, within PGA1_c35620 at 3735.486 kb on - strand, within PGA1_c35620 at 3735.488 kb on - strand, within PGA1_c35620 at 3735.510 kb on - strand, within PGA1_c35620 at 3735.511 kb on - strand, within PGA1_c35620 at 3735.612 kb on + strand at 3735.612 kb on + strand at 3735.629 kb on + strand at 3735.646 kb on + strand at 3735.654 kb on - strand at 3735.654 kb on - strand at 3735.666 kb on + strand, within PGA1_c35630 at 3735.666 kb on + strand, within PGA1_c35630 at 3735.674 kb on - strand, within PGA1_c35630 at 3735.674 kb on - strand, within PGA1_c35630 at 3735.710 kb on - strand, within PGA1_c35630 at 3735.710 kb on - strand, within PGA1_c35630 at 3735.717 kb on + strand, within PGA1_c35630 at 3735.720 kb on - strand, within PGA1_c35630 at 3735.720 kb on - strand, within PGA1_c35630 at 3735.725 kb on - strand, within PGA1_c35630 at 3735.725 kb on - strand, within PGA1_c35630 at 3735.727 kb on - strand, within PGA1_c35630 at 3735.789 kb on + strand, within PGA1_c35630 at 3735.797 kb on + strand, within PGA1_c35630 at 3735.805 kb on - strand, within PGA1_c35630 at 3735.842 kb on + strand, within PGA1_c35630 at 3735.842 kb on + strand, within PGA1_c35630 at 3735.914 kb on + strand, within PGA1_c35630 at 3735.917 kb on - strand, within PGA1_c35630 at 3735.917 kb on - strand, within PGA1_c35630 at 3735.917 kb on - strand, within PGA1_c35630 at 3735.919 kb on + strand, within PGA1_c35630 at 3735.919 kb on + strand, within PGA1_c35630 at 3735.919 kb on + strand, within PGA1_c35630 at 3735.919 kb on + strand, within PGA1_c35630 at 3735.922 kb on - strand, within PGA1_c35630 at 3735.927 kb on - strand, within PGA1_c35630 at 3735.927 kb on - strand, within PGA1_c35630 at 3736.001 kb on - strand, within PGA1_c35630 at 3736.011 kb on + strand, within PGA1_c35630 at 3736.011 kb on + strand, within PGA1_c35630 at 3736.011 kb on + strand, within PGA1_c35630 at 3736.019 kb on - strand, within PGA1_c35630 at 3736.019 kb on - strand, within PGA1_c35630 at 3736.019 kb on - strand, within PGA1_c35630 at 3736.019 kb on - strand, within PGA1_c35630 at 3736.019 kb on - strand, within PGA1_c35630 at 3736.019 kb on - strand, within PGA1_c35630 at 3736.019 kb on - strand, within PGA1_c35630 at 3736.019 kb on - strand, within PGA1_c35630 at 3736.019 kb on - strand, within PGA1_c35630 at 3736.031 kb on - strand, within PGA1_c35630 at 3736.035 kb on + strand, within PGA1_c35630 at 3736.041 kb on - strand, within PGA1_c35630 at 3736.041 kb on - strand, within PGA1_c35630 at 3736.056 kb on + strand, within PGA1_c35630 at 3736.060 kb on + strand, within PGA1_c35630 at 3736.060 kb on + strand, within PGA1_c35630 at 3736.060 kb on + strand, within PGA1_c35630 at 3736.068 kb on - strand, within PGA1_c35630 at 3736.072 kb on + strand, within PGA1_c35630 at 3736.139 kb on + strand at 3736.211 kb on + strand at 3736.233 kb on - strand at 3736.248 kb on - strand at 3736.293 kb on - strand at 3736.307 kb on + strand at 3736.316 kb on - strand at 3736.323 kb on + strand at 3736.328 kb on - strand at 3736.348 kb on + strand at 3736.349 kb on - strand at 3736.351 kb on - strand at 3736.355 kb on + strand at 3736.356 kb on - strand at 3736.387 kb on + strand, within PGA1_c35640 at 3736.387 kb on + strand, within PGA1_c35640 at 3736.387 kb on + strand, within PGA1_c35640 at 3736.387 kb on + strand, within PGA1_c35640 at 3736.388 kb on - strand, within PGA1_c35640 at 3736.395 kb on - strand, within PGA1_c35640 at 3736.402 kb on + strand, within PGA1_c35640 at 3736.428 kb on - strand, within PGA1_c35640 at 3736.429 kb on + strand, within PGA1_c35640 at 3736.577 kb on + strand, within PGA1_c35640 at 3736.580 kb on - strand, within PGA1_c35640 at 3736.601 kb on - strand, within PGA1_c35640
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 3,734,366 + +0.4 3,734,388 + +0.2 3,734,420 + +0.9 3,734,420 + -0.9 3,734,438 - -0.9 3,734,438 - -0.5 3,734,455 + +0.6 3,734,463 - -0.9 3,734,463 - +0.7 3,734,515 + +0.0 3,734,517 + -0.1 3,734,518 - -0.8 3,734,604 + -0.9 3,734,604 + -0.8 3,734,604 + -0.6 3,734,612 - -3.1 3,734,612 - -1.0 3,734,612 - -1.7 3,734,681 - PGA1_c35610 0.15 -1.2 3,734,694 + PGA1_c35610 0.17 -0.4 3,734,728 - PGA1_c35610 0.22 -1.1 3,734,751 + PGA1_c35610 0.25 -1.0 3,734,759 - PGA1_c35610 0.26 -2.8 3,734,778 + PGA1_c35610 0.28 -0.6 3,734,793 - PGA1_c35610 0.31 -1.4 3,734,809 + PGA1_c35610 0.33 -0.5 3,734,831 - PGA1_c35610 0.36 -1.4 3,734,832 + PGA1_c35610 0.36 -2.4 3,734,837 + PGA1_c35610 0.36 -2.0 3,734,837 + PGA1_c35610 0.36 -0.5 3,734,876 + PGA1_c35610 0.42 -1.1 3,734,899 - PGA1_c35610 0.45 -0.2 3,734,899 - PGA1_c35610 0.45 +1.1 3,734,899 - PGA1_c35610 0.45 +0.3 3,734,899 - PGA1_c35610 0.45 -0.0 3,734,909 - PGA1_c35610 0.46 -0.8 3,734,977 + PGA1_c35610 0.55 +0.0 3,734,986 - PGA1_c35610 0.56 -0.4 3,735,027 + PGA1_c35610 0.62 -0.1 3,735,027 + PGA1_c35610 0.62 -0.9 3,735,027 + PGA1_c35610 0.62 -0.5 3,735,027 + PGA1_c35610 0.62 +0.3 3,735,035 - PGA1_c35610 0.63 -2.4 3,735,035 - PGA1_c35610 0.63 -1.3 3,735,035 - PGA1_c35610 0.63 -3.5 3,735,035 - PGA1_c35610 0.63 -1.4 3,735,035 - PGA1_c35610 0.63 -2.6 3,735,041 + PGA1_c35610 0.64 +0.1 3,735,071 + PGA1_c35610 0.68 -2.5 3,735,071 + PGA1_c35610 0.68 -3.3 3,735,138 + PGA1_c35610 0.77 -2.4 3,735,138 + PGA1_c35610 0.77 -0.9 3,735,146 - PGA1_c35610 0.78 -1.5 3,735,146 - PGA1_c35610 0.78 -0.3 3,735,152 - PGA1_c35610 0.79 -1.8 3,735,185 - PGA1_c35610 0.83 -1.4 3,735,194 + PGA1_c35610 0.84 -1.4 3,735,194 + PGA1_c35610 0.84 -0.9 3,735,202 - PGA1_c35610 0.85 -1.6 3,735,216 + PGA1_c35610 0.87 -1.1 3,735,303 + -1.7 3,735,344 + PGA1_c35620 0.13 -1.6 3,735,352 - PGA1_c35620 0.15 +0.2 3,735,352 - PGA1_c35620 0.15 -2.5 3,735,354 + PGA1_c35620 0.16 -0.2 3,735,395 - PGA1_c35620 0.30 -1.6 3,735,411 + PGA1_c35620 0.35 -1.0 3,735,421 - PGA1_c35620 0.39 -2.2 3,735,479 + PGA1_c35620 0.58 +0.0 3,735,486 - PGA1_c35620 0.61 -0.7 3,735,488 - PGA1_c35620 0.61 -1.6 3,735,510 - PGA1_c35620 0.69 +0.2 3,735,511 - PGA1_c35620 0.69 -0.6 3,735,612 + -0.8 3,735,612 + -0.8 3,735,629 + -0.5 3,735,646 + +0.0 3,735,654 - -0.3 3,735,654 - -0.7 3,735,666 + PGA1_c35630 0.12 -0.8 3,735,666 + PGA1_c35630 0.12 -1.4 3,735,674 - PGA1_c35630 0.13 -1.7 3,735,674 - PGA1_c35630 0.13 -2.1 3,735,710 - PGA1_c35630 0.19 -1.9 3,735,710 - PGA1_c35630 0.19 -0.9 3,735,717 + PGA1_c35630 0.20 -0.6 3,735,720 - PGA1_c35630 0.21 -0.9 3,735,720 - PGA1_c35630 0.21 -1.7 3,735,725 - PGA1_c35630 0.22 -0.5 3,735,725 - PGA1_c35630 0.22 -1.5 3,735,727 - PGA1_c35630 0.22 -1.1 3,735,789 + PGA1_c35630 0.32 -1.5 3,735,797 + PGA1_c35630 0.34 -0.4 3,735,805 - PGA1_c35630 0.35 -1.7 3,735,842 + PGA1_c35630 0.41 -1.3 3,735,842 + PGA1_c35630 0.41 -0.4 3,735,914 + PGA1_c35630 0.53 -1.6 3,735,917 - PGA1_c35630 0.54 -2.2 3,735,917 - PGA1_c35630 0.54 -0.9 3,735,917 - PGA1_c35630 0.54 -1.2 3,735,919 + PGA1_c35630 0.54 -0.4 3,735,919 + PGA1_c35630 0.54 -0.9 3,735,919 + PGA1_c35630 0.54 +0.7 3,735,919 + PGA1_c35630 0.54 -2.4 3,735,922 - PGA1_c35630 0.55 -1.7 3,735,927 - PGA1_c35630 0.56 -0.2 3,735,927 - PGA1_c35630 0.56 -1.8 3,736,001 - PGA1_c35630 0.68 -0.5 3,736,011 + PGA1_c35630 0.70 -1.1 3,736,011 + PGA1_c35630 0.70 -0.3 3,736,011 + PGA1_c35630 0.70 -1.6 3,736,019 - PGA1_c35630 0.71 -1.3 3,736,019 - PGA1_c35630 0.71 -1.0 3,736,019 - PGA1_c35630 0.71 -1.7 3,736,019 - PGA1_c35630 0.71 -1.0 3,736,019 - PGA1_c35630 0.71 -2.2 3,736,019 - PGA1_c35630 0.71 -0.9 3,736,019 - PGA1_c35630 0.71 -1.2 3,736,019 - PGA1_c35630 0.71 -0.6 3,736,019 - PGA1_c35630 0.71 -1.7 3,736,031 - PGA1_c35630 0.73 -1.9 3,736,035 + PGA1_c35630 0.74 -0.7 3,736,041 - PGA1_c35630 0.75 -0.4 3,736,041 - PGA1_c35630 0.75 +0.2 3,736,056 + PGA1_c35630 0.77 -0.8 3,736,060 + PGA1_c35630 0.78 -2.5 3,736,060 + PGA1_c35630 0.78 -1.4 3,736,060 + PGA1_c35630 0.78 -0.8 3,736,068 - PGA1_c35630 0.79 -1.2 3,736,072 + PGA1_c35630 0.80 -0.1 3,736,139 + +0.3 3,736,211 + +1.0 3,736,233 - -0.7 3,736,248 - -2.4 3,736,293 - -1.0 3,736,307 + -0.0 3,736,316 - -1.3 3,736,323 + -0.7 3,736,328 - -1.0 3,736,348 + -0.9 3,736,349 - -1.9 3,736,351 - -1.3 3,736,355 + -0.4 3,736,356 - -2.0 3,736,387 + PGA1_c35640 0.12 -1.1 3,736,387 + PGA1_c35640 0.12 +0.0 3,736,387 + PGA1_c35640 0.12 -0.6 3,736,387 + PGA1_c35640 0.12 -0.0 3,736,388 - PGA1_c35640 0.12 -1.3 3,736,395 - PGA1_c35640 0.12 -0.8 3,736,402 + PGA1_c35640 0.13 -1.7 3,736,428 - PGA1_c35640 0.14 +0.8 3,736,429 + PGA1_c35640 0.14 -1.5 3,736,577 + PGA1_c35640 0.23 -1.1 3,736,580 - PGA1_c35640 0.23 -0.7 3,736,601 - PGA1_c35640 0.24 -2.1
Or see this region's nucleotide sequence