Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c34920 and PGA1_c34930 overlap by 4 nucleotides PGA1_c34930 and PGA1_c34940 are separated by 92 nucleotides PGA1_c34940 and PGA1_c34950 are separated by 89 nucleotides
PGA1_c34920: PGA1_c34920 - argininosuccinate synthase ArgG, at 3,664,269 to 3,665,495
_c34920
PGA1_c34930: PGA1_c34930 - Uncharacterized conserved protein, at 3,665,492 to 3,665,950
_c34930
PGA1_c34940: PGA1_c34940 - Putative sterol carrier protein, at 3,666,043 to 3,666,327
_c34940
PGA1_c34950: PGA1_c34950 - putative branched-chain-amino-acid aminotransferase, subunit B, at 3,666,417 to 3,667,130
_c34950
Position (kb)
3665
3666 Strain fitness (log2 ratio)
-1
0
1
2 at 3664.538 kb on - strand, within PGA1_c34920 at 3665.576 kb on + strand, within PGA1_c34930 at 3665.668 kb on + strand, within PGA1_c34930 at 3665.686 kb on - strand, within PGA1_c34930 at 3665.691 kb on - strand, within PGA1_c34930 at 3665.735 kb on + strand, within PGA1_c34930 at 3665.735 kb on + strand, within PGA1_c34930 at 3665.755 kb on - strand, within PGA1_c34930 at 3665.778 kb on + strand, within PGA1_c34930 at 3665.778 kb on + strand, within PGA1_c34930 at 3665.778 kb on + strand, within PGA1_c34930 at 3665.778 kb on + strand, within PGA1_c34930 at 3665.778 kb on + strand, within PGA1_c34930 at 3665.786 kb on - strand, within PGA1_c34930 at 3665.786 kb on - strand, within PGA1_c34930 at 3665.786 kb on - strand, within PGA1_c34930 at 3665.800 kb on + strand, within PGA1_c34930 at 3665.849 kb on - strand, within PGA1_c34930 at 3665.851 kb on - strand, within PGA1_c34930 at 3665.881 kb on + strand, within PGA1_c34930 at 3665.889 kb on - strand, within PGA1_c34930 at 3665.914 kb on - strand at 3665.917 kb on + strand at 3665.917 kb on + strand at 3665.925 kb on - strand at 3665.925 kb on - strand at 3665.931 kb on + strand at 3665.959 kb on - strand at 3665.977 kb on + strand at 3666.000 kb on + strand at 3666.001 kb on + strand at 3666.001 kb on + strand at 3666.001 kb on + strand at 3666.001 kb on + strand at 3666.009 kb on - strand at 3666.009 kb on - strand at 3666.009 kb on - strand at 3666.009 kb on - strand at 3666.009 kb on - strand at 3666.009 kb on - strand at 3666.009 kb on - strand at 3666.047 kb on - strand at 3666.074 kb on + strand, within PGA1_c34940 at 3666.074 kb on + strand, within PGA1_c34940 at 3666.144 kb on - strand, within PGA1_c34940 at 3666.182 kb on - strand, within PGA1_c34940 at 3666.191 kb on - strand, within PGA1_c34940 at 3666.193 kb on + strand, within PGA1_c34940 at 3666.206 kb on - strand, within PGA1_c34940 at 3666.292 kb on + strand, within PGA1_c34940 at 3666.323 kb on + strand at 3666.345 kb on + strand at 3666.404 kb on + strand at 3666.406 kb on + strand at 3666.406 kb on + strand at 3666.406 kb on + strand at 3666.440 kb on + strand at 3666.448 kb on - strand at 3666.448 kb on - strand at 3666.458 kb on - strand at 3666.473 kb on + strand at 3666.489 kb on - strand, within PGA1_c34950 at 3666.531 kb on + strand, within PGA1_c34950 at 3666.549 kb on - strand, within PGA1_c34950 at 3666.629 kb on + strand, within PGA1_c34950 at 3666.629 kb on + strand, within PGA1_c34950 at 3666.687 kb on - strand, within PGA1_c34950 at 3666.729 kb on + strand, within PGA1_c34950 at 3666.842 kb on + strand, within PGA1_c34950 at 3666.871 kb on + strand, within PGA1_c34950 at 3666.871 kb on + strand, within PGA1_c34950 at 3666.871 kb on + strand, within PGA1_c34950 at 3666.871 kb on + strand, within PGA1_c34950 at 3666.878 kb on + strand, within PGA1_c34950 at 3666.883 kb on + strand, within PGA1_c34950 at 3666.938 kb on - strand, within PGA1_c34950
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 3,664,538 - PGA1_c34920 0.22 +1.1 3,665,576 + PGA1_c34930 0.18 +0.3 3,665,668 + PGA1_c34930 0.38 +0.8 3,665,686 - PGA1_c34930 0.42 -0.3 3,665,691 - PGA1_c34930 0.43 +0.5 3,665,735 + PGA1_c34930 0.53 +0.8 3,665,735 + PGA1_c34930 0.53 +0.5 3,665,755 - PGA1_c34930 0.57 -0.0 3,665,778 + PGA1_c34930 0.62 -1.3 3,665,778 + PGA1_c34930 0.62 +1.0 3,665,778 + PGA1_c34930 0.62 +0.1 3,665,778 + PGA1_c34930 0.62 +0.3 3,665,778 + PGA1_c34930 0.62 +0.3 3,665,786 - PGA1_c34930 0.64 +0.1 3,665,786 - PGA1_c34930 0.64 +0.7 3,665,786 - PGA1_c34930 0.64 +1.7 3,665,800 + PGA1_c34930 0.67 -1.5 3,665,849 - PGA1_c34930 0.78 +0.7 3,665,851 - PGA1_c34930 0.78 -1.1 3,665,881 + PGA1_c34930 0.85 +0.8 3,665,889 - PGA1_c34930 0.86 -0.1 3,665,914 - +0.3 3,665,917 + +0.8 3,665,917 + -0.3 3,665,925 - +0.4 3,665,925 - +0.7 3,665,931 + -1.1 3,665,959 - -0.0 3,665,977 + +0.3 3,666,000 + -1.5 3,666,001 + +0.3 3,666,001 + -0.3 3,666,001 + +0.3 3,666,001 + -0.1 3,666,009 - +0.9 3,666,009 - +0.3 3,666,009 - +1.8 3,666,009 - -0.6 3,666,009 - +0.3 3,666,009 - -0.4 3,666,009 - +0.0 3,666,047 - -0.8 3,666,074 + PGA1_c34940 0.11 +0.3 3,666,074 + PGA1_c34940 0.11 +1.0 3,666,144 - PGA1_c34940 0.35 +0.4 3,666,182 - PGA1_c34940 0.49 +0.6 3,666,191 - PGA1_c34940 0.52 -1.2 3,666,193 + PGA1_c34940 0.53 +0.1 3,666,206 - PGA1_c34940 0.57 -0.6 3,666,292 + PGA1_c34940 0.87 -1.5 3,666,323 + -0.3 3,666,345 + -1.0 3,666,404 + -1.3 3,666,406 + -0.6 3,666,406 + -0.3 3,666,406 + +0.2 3,666,440 + +0.4 3,666,448 - +0.5 3,666,448 - -0.8 3,666,458 - +0.2 3,666,473 + +0.5 3,666,489 - PGA1_c34950 0.10 -0.1 3,666,531 + PGA1_c34950 0.16 +0.7 3,666,549 - PGA1_c34950 0.18 +0.7 3,666,629 + PGA1_c34950 0.30 +0.1 3,666,629 + PGA1_c34950 0.30 -0.0 3,666,687 - PGA1_c34950 0.38 -0.5 3,666,729 + PGA1_c34950 0.44 +1.5 3,666,842 + PGA1_c34950 0.60 -1.0 3,666,871 + PGA1_c34950 0.64 +0.1 3,666,871 + PGA1_c34950 0.64 +0.0 3,666,871 + PGA1_c34950 0.64 -0.1 3,666,871 + PGA1_c34950 0.64 -0.5 3,666,878 + PGA1_c34950 0.65 -0.1 3,666,883 + PGA1_c34950 0.65 +1.1 3,666,938 - PGA1_c34950 0.73 +0.6
Or see this region's nucleotide sequence