Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c26710 and PGA1_c26720 are separated by 98 nucleotides PGA1_c26720 and PGA1_c26730 are separated by 2 nucleotides PGA1_c26730 and PGA1_c26740 are separated by 12 nucleotides
PGA1_c26710: PGA1_c26710 - glutathione synthetase GshB, at 2,785,944 to 2,786,876
_c26710
PGA1_c26720: PGA1_c26720 - Predicted endonuclease distantly related to archaeal Holliday junction resolvase, at 2,786,975 to 2,787,379
_c26720
PGA1_c26730: PGA1_c26730 - probable S-adenosylmethionine-dependent methyltransferase, YraL family, at 2,787,382 to 2,788,242
_c26730
PGA1_c26740: PGA1_c26740 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 2,788,255 to 2,789,499
_c26740
Position (kb)
2786
2787
2788 Strain fitness (log2 ratio)
-1
0
1 at 2786.896 kb on - strand at 2786.973 kb on + strand at 2786.973 kb on + strand at 2786.978 kb on + strand at 2786.981 kb on - strand at 2786.986 kb on - strand at 2786.991 kb on - strand at 2787.012 kb on - strand at 2787.108 kb on + strand, within PGA1_c26720 at 2787.123 kb on + strand, within PGA1_c26720 at 2787.123 kb on + strand, within PGA1_c26720 at 2787.123 kb on + strand, within PGA1_c26720 at 2787.141 kb on + strand, within PGA1_c26720 at 2787.169 kb on - strand, within PGA1_c26720 at 2787.265 kb on + strand, within PGA1_c26720 at 2787.751 kb on + strand, within PGA1_c26730 at 2787.874 kb on + strand, within PGA1_c26730 at 2787.895 kb on - strand, within PGA1_c26730 at 2787.970 kb on + strand, within PGA1_c26730 at 2787.970 kb on + strand, within PGA1_c26730 at 2787.997 kb on - strand, within PGA1_c26730 at 2788.012 kb on - strand, within PGA1_c26730 at 2788.028 kb on + strand, within PGA1_c26730 at 2788.031 kb on - strand, within PGA1_c26730 at 2788.072 kb on - strand, within PGA1_c26730 at 2788.112 kb on - strand, within PGA1_c26730 at 2788.166 kb on - strand at 2788.181 kb on + strand at 2788.181 kb on + strand at 2788.279 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 2,786,896 - -0.6 2,786,973 + +0.2 2,786,973 + +0.4 2,786,978 + -1.0 2,786,981 - -0.9 2,786,986 - -0.2 2,786,991 - +0.2 2,787,012 - +1.0 2,787,108 + PGA1_c26720 0.33 -1.2 2,787,123 + PGA1_c26720 0.37 -0.2 2,787,123 + PGA1_c26720 0.37 -0.1 2,787,123 + PGA1_c26720 0.37 -0.9 2,787,141 + PGA1_c26720 0.41 -0.5 2,787,169 - PGA1_c26720 0.48 -1.3 2,787,265 + PGA1_c26720 0.72 -0.2 2,787,751 + PGA1_c26730 0.43 -1.7 2,787,874 + PGA1_c26730 0.57 +0.5 2,787,895 - PGA1_c26730 0.60 +0.7 2,787,970 + PGA1_c26730 0.68 -1.6 2,787,970 + PGA1_c26730 0.68 -0.5 2,787,997 - PGA1_c26730 0.71 +0.1 2,788,012 - PGA1_c26730 0.73 -0.8 2,788,028 + PGA1_c26730 0.75 +0.2 2,788,031 - PGA1_c26730 0.75 -1.2 2,788,072 - PGA1_c26730 0.80 -0.4 2,788,112 - PGA1_c26730 0.85 -0.3 2,788,166 - +0.4 2,788,181 + +0.8 2,788,181 + -0.0 2,788,279 + -0.8
Or see this region's nucleotide sequence