Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02430 and PGA1_c02440 overlap by 4 nucleotides PGA1_c02440 and PGA1_c02450 overlap by 4 nucleotides
PGA1_c02430: PGA1_c02430 - type I secretion membrane fusion protein, HlyD family, at 219,676 to 220,845
_c02430
PGA1_c02440: PGA1_c02440 - putative ATP-binding/permease fusion ABC transporter, at 220,842 to 223,208
_c02440
PGA1_c02450: PGA1_c02450 - putative outer membrane efflux protein, at 223,205 to 224,524
_c02450
Position (kb) 220
221
222
223
224 Strain fitness (log2 ratio) -1
0
1
2
3 at 219.852 kb on - strand, within PGA1_c02430 at 219.931 kb on + strand, within PGA1_c02430 at 219.937 kb on + strand, within PGA1_c02430 at 219.945 kb on - strand, within PGA1_c02430 at 219.973 kb on + strand, within PGA1_c02430 at 219.991 kb on - strand, within PGA1_c02430 at 220.006 kb on - strand, within PGA1_c02430 at 220.006 kb on - strand, within PGA1_c02430 at 220.221 kb on + strand, within PGA1_c02430 at 220.221 kb on + strand, within PGA1_c02430 at 220.221 kb on + strand, within PGA1_c02430 at 220.295 kb on + strand, within PGA1_c02430 at 220.303 kb on - strand, within PGA1_c02430 at 220.305 kb on + strand, within PGA1_c02430 at 220.337 kb on + strand, within PGA1_c02430 at 220.345 kb on - strand, within PGA1_c02430 at 220.415 kb on + strand, within PGA1_c02430 at 220.415 kb on + strand, within PGA1_c02430 at 220.423 kb on - strand, within PGA1_c02430 at 220.436 kb on + strand, within PGA1_c02430 at 220.439 kb on - strand, within PGA1_c02430 at 220.439 kb on - strand, within PGA1_c02430 at 220.597 kb on + strand, within PGA1_c02430 at 220.603 kb on + strand, within PGA1_c02430 at 220.611 kb on - strand, within PGA1_c02430 at 220.613 kb on + strand, within PGA1_c02430 at 220.613 kb on + strand, within PGA1_c02430 at 220.621 kb on - strand, within PGA1_c02430 at 220.622 kb on + strand, within PGA1_c02430 at 220.622 kb on + strand, within PGA1_c02430 at 220.627 kb on + strand, within PGA1_c02430 at 220.630 kb on - strand, within PGA1_c02430 at 220.636 kb on - strand, within PGA1_c02430 at 220.652 kb on - strand, within PGA1_c02430 at 220.696 kb on + strand, within PGA1_c02430 at 220.704 kb on - strand, within PGA1_c02430 at 220.737 kb on + strand at 220.832 kb on - strand at 220.870 kb on + strand at 220.893 kb on + strand at 220.893 kb on + strand at 220.893 kb on + strand at 220.893 kb on + strand at 221.050 kb on + strand at 221.052 kb on - strand at 221.092 kb on - strand, within PGA1_c02440 at 221.092 kb on - strand, within PGA1_c02440 at 221.178 kb on + strand, within PGA1_c02440 at 221.189 kb on + strand, within PGA1_c02440 at 221.223 kb on - strand, within PGA1_c02440 at 221.246 kb on + strand, within PGA1_c02440 at 221.253 kb on - strand, within PGA1_c02440 at 221.299 kb on - strand, within PGA1_c02440 at 221.299 kb on - strand, within PGA1_c02440 at 221.333 kb on + strand, within PGA1_c02440 at 221.343 kb on + strand, within PGA1_c02440 at 221.351 kb on - strand, within PGA1_c02440 at 221.356 kb on - strand, within PGA1_c02440 at 221.404 kb on + strand, within PGA1_c02440 at 221.404 kb on + strand, within PGA1_c02440 at 221.414 kb on + strand, within PGA1_c02440 at 221.414 kb on + strand, within PGA1_c02440 at 221.414 kb on + strand, within PGA1_c02440 at 221.414 kb on + strand, within PGA1_c02440 at 221.414 kb on + strand, within PGA1_c02440 at 221.414 kb on + strand, within PGA1_c02440 at 221.414 kb on + strand, within PGA1_c02440 at 221.414 kb on + strand, within PGA1_c02440 at 221.422 kb on - strand, within PGA1_c02440 at 221.422 kb on - strand, within PGA1_c02440 at 221.422 kb on - strand, within PGA1_c02440 at 221.423 kb on + strand, within PGA1_c02440 at 221.459 kb on + strand, within PGA1_c02440 at 221.459 kb on + strand, within PGA1_c02440 at 221.459 kb on + strand, within PGA1_c02440 at 221.459 kb on + strand, within PGA1_c02440 at 221.520 kb on + strand, within PGA1_c02440 at 221.528 kb on - strand, within PGA1_c02440 at 221.635 kb on + strand, within PGA1_c02440 at 221.635 kb on + strand, within PGA1_c02440 at 221.643 kb on - strand, within PGA1_c02440 at 221.643 kb on - strand, within PGA1_c02440 at 221.654 kb on - strand, within PGA1_c02440 at 221.690 kb on + strand, within PGA1_c02440 at 221.698 kb on - strand, within PGA1_c02440 at 221.708 kb on - strand, within PGA1_c02440 at 221.848 kb on + strand, within PGA1_c02440 at 221.945 kb on - strand, within PGA1_c02440 at 221.955 kb on - strand, within PGA1_c02440 at 221.973 kb on - strand, within PGA1_c02440 at 222.004 kb on + strand, within PGA1_c02440 at 222.007 kb on - strand, within PGA1_c02440 at 222.007 kb on - strand, within PGA1_c02440 at 222.052 kb on - strand, within PGA1_c02440 at 222.120 kb on - strand, within PGA1_c02440 at 222.122 kb on - strand, within PGA1_c02440 at 222.154 kb on + strand, within PGA1_c02440 at 222.260 kb on - strand, within PGA1_c02440 at 222.261 kb on + strand, within PGA1_c02440 at 222.269 kb on - strand, within PGA1_c02440 at 222.319 kb on + strand, within PGA1_c02440 at 222.327 kb on - strand, within PGA1_c02440 at 222.387 kb on + strand, within PGA1_c02440 at 222.390 kb on - strand, within PGA1_c02440 at 222.395 kb on - strand, within PGA1_c02440 at 222.395 kb on - strand, within PGA1_c02440 at 222.401 kb on - strand, within PGA1_c02440 at 222.408 kb on + strand, within PGA1_c02440 at 222.416 kb on + strand, within PGA1_c02440 at 222.416 kb on + strand, within PGA1_c02440 at 222.416 kb on - strand, within PGA1_c02440 at 222.416 kb on - strand, within PGA1_c02440 at 222.419 kb on + strand, within PGA1_c02440 at 222.422 kb on - strand, within PGA1_c02440 at 222.424 kb on - strand, within PGA1_c02440 at 222.461 kb on + strand, within PGA1_c02440 at 222.461 kb on + strand, within PGA1_c02440 at 222.532 kb on + strand, within PGA1_c02440 at 222.598 kb on - strand, within PGA1_c02440 at 222.598 kb on - strand, within PGA1_c02440 at 222.611 kb on + strand, within PGA1_c02440 at 222.704 kb on - strand, within PGA1_c02440 at 222.790 kb on - strand, within PGA1_c02440 at 222.819 kb on + strand, within PGA1_c02440 at 222.830 kb on + strand, within PGA1_c02440 at 222.897 kb on + strand, within PGA1_c02440 at 222.958 kb on + strand, within PGA1_c02440 at 222.966 kb on - strand, within PGA1_c02440 at 222.966 kb on - strand, within PGA1_c02440 at 223.030 kb on - strand at 223.062 kb on - strand at 223.094 kb on - strand at 223.094 kb on - strand at 223.125 kb on - strand at 223.184 kb on - strand at 223.186 kb on + strand at 223.203 kb on + strand at 223.254 kb on + strand at 223.267 kb on - strand at 223.312 kb on + strand at 223.315 kb on - strand at 223.341 kb on + strand, within PGA1_c02450 at 223.354 kb on - strand, within PGA1_c02450 at 223.356 kb on + strand, within PGA1_c02450 at 223.356 kb on + strand, within PGA1_c02450 at 223.356 kb on + strand, within PGA1_c02450 at 223.356 kb on + strand, within PGA1_c02450 at 223.356 kb on + strand, within PGA1_c02450 at 223.356 kb on + strand, within PGA1_c02450 at 223.356 kb on + strand, within PGA1_c02450 at 223.356 kb on + strand, within PGA1_c02450 at 223.356 kb on + strand, within PGA1_c02450 at 223.364 kb on - strand, within PGA1_c02450 at 223.364 kb on - strand, within PGA1_c02450 at 223.364 kb on - strand, within PGA1_c02450 at 223.364 kb on - strand, within PGA1_c02450 at 223.364 kb on - strand, within PGA1_c02450 at 223.364 kb on - strand, within PGA1_c02450 at 223.410 kb on - strand, within PGA1_c02450 at 223.410 kb on - strand, within PGA1_c02450 at 223.444 kb on - strand, within PGA1_c02450 at 223.499 kb on + strand, within PGA1_c02450 at 223.569 kb on - strand, within PGA1_c02450 at 223.579 kb on + strand, within PGA1_c02450 at 223.587 kb on - strand, within PGA1_c02450 at 223.605 kb on - strand, within PGA1_c02450 at 223.606 kb on + strand, within PGA1_c02450 at 223.634 kb on + strand, within PGA1_c02450 at 223.640 kb on - strand, within PGA1_c02450 at 223.670 kb on - strand, within PGA1_c02450 at 223.670 kb on - strand, within PGA1_c02450 at 223.672 kb on + strand, within PGA1_c02450 at 223.672 kb on + strand, within PGA1_c02450 at 223.680 kb on - strand, within PGA1_c02450 at 223.680 kb on - strand, within PGA1_c02450 at 223.680 kb on - strand, within PGA1_c02450 at 223.700 kb on + strand, within PGA1_c02450 at 223.742 kb on + strand, within PGA1_c02450 at 223.747 kb on + strand, within PGA1_c02450 at 223.843 kb on - strand, within PGA1_c02450 at 223.872 kb on + strand, within PGA1_c02450 at 223.873 kb on - strand, within PGA1_c02450 at 223.892 kb on + strand, within PGA1_c02450 at 223.900 kb on - strand, within PGA1_c02450 at 223.924 kb on - strand, within PGA1_c02450 at 223.962 kb on - strand, within PGA1_c02450 at 223.962 kb on - strand, within PGA1_c02450 at 223.962 kb on - strand, within PGA1_c02450 at 224.002 kb on + strand, within PGA1_c02450 at 224.002 kb on + strand, within PGA1_c02450 at 224.002 kb on + strand, within PGA1_c02450 at 224.002 kb on + strand, within PGA1_c02450 at 224.010 kb on - strand, within PGA1_c02450 at 224.010 kb on - strand, within PGA1_c02450 at 224.010 kb on - strand, within PGA1_c02450 at 224.010 kb on - strand, within PGA1_c02450 at 224.010 kb on - strand, within PGA1_c02450 at 224.010 kb on - strand, within PGA1_c02450 at 224.019 kb on + strand, within PGA1_c02450 at 224.027 kb on - strand, within PGA1_c02450 at 224.050 kb on + strand, within PGA1_c02450
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 219,852 - PGA1_c02430 0.15 +1.7 219,931 + PGA1_c02430 0.22 +1.2 219,937 + PGA1_c02430 0.22 +1.0 219,945 - PGA1_c02430 0.23 +0.4 219,973 + PGA1_c02430 0.25 +1.6 219,991 - PGA1_c02430 0.27 +1.8 220,006 - PGA1_c02430 0.28 +1.3 220,006 - PGA1_c02430 0.28 +1.7 220,221 + PGA1_c02430 0.47 +1.6 220,221 + PGA1_c02430 0.47 +1.0 220,221 + PGA1_c02430 0.47 +2.4 220,295 + PGA1_c02430 0.53 +1.3 220,303 - PGA1_c02430 0.54 +0.9 220,305 + PGA1_c02430 0.54 +1.5 220,337 + PGA1_c02430 0.56 +1.0 220,345 - PGA1_c02430 0.57 +2.1 220,415 + PGA1_c02430 0.63 +1.6 220,415 + PGA1_c02430 0.63 +1.6 220,423 - PGA1_c02430 0.64 +1.5 220,436 + PGA1_c02430 0.65 +1.5 220,439 - PGA1_c02430 0.65 +1.8 220,439 - PGA1_c02430 0.65 +1.8 220,597 + PGA1_c02430 0.79 +1.8 220,603 + PGA1_c02430 0.79 +1.6 220,611 - PGA1_c02430 0.80 +0.6 220,613 + PGA1_c02430 0.80 +0.7 220,613 + PGA1_c02430 0.80 +1.6 220,621 - PGA1_c02430 0.81 +1.7 220,622 + PGA1_c02430 0.81 +2.2 220,622 + PGA1_c02430 0.81 +2.2 220,627 + PGA1_c02430 0.81 +1.2 220,630 - PGA1_c02430 0.82 +0.9 220,636 - PGA1_c02430 0.82 +1.2 220,652 - PGA1_c02430 0.83 +1.9 220,696 + PGA1_c02430 0.87 +2.3 220,704 - PGA1_c02430 0.88 +2.5 220,737 + +1.3 220,832 - +2.3 220,870 + +1.5 220,893 + +2.0 220,893 + +2.0 220,893 + +1.3 220,893 + +0.9 221,050 + +2.1 221,052 - +1.5 221,092 - PGA1_c02440 0.11 +1.5 221,092 - PGA1_c02440 0.11 +1.0 221,178 + PGA1_c02440 0.14 +0.6 221,189 + PGA1_c02440 0.15 +2.2 221,223 - PGA1_c02440 0.16 +1.7 221,246 + PGA1_c02440 0.17 +1.5 221,253 - PGA1_c02440 0.17 +1.3 221,299 - PGA1_c02440 0.19 +0.8 221,299 - PGA1_c02440 0.19 +1.2 221,333 + PGA1_c02440 0.21 +2.3 221,343 + PGA1_c02440 0.21 +2.0 221,351 - PGA1_c02440 0.22 +1.2 221,356 - PGA1_c02440 0.22 +2.5 221,404 + PGA1_c02440 0.24 +1.9 221,404 + PGA1_c02440 0.24 +1.5 221,414 + PGA1_c02440 0.24 +2.2 221,414 + PGA1_c02440 0.24 +1.8 221,414 + PGA1_c02440 0.24 +2.2 221,414 + PGA1_c02440 0.24 +1.4 221,414 + PGA1_c02440 0.24 +1.1 221,414 + PGA1_c02440 0.24 +1.1 221,414 + PGA1_c02440 0.24 +1.7 221,414 + PGA1_c02440 0.24 +1.5 221,422 - PGA1_c02440 0.25 +1.2 221,422 - PGA1_c02440 0.25 +1.1 221,422 - PGA1_c02440 0.25 +1.3 221,423 + PGA1_c02440 0.25 +1.6 221,459 + PGA1_c02440 0.26 +1.6 221,459 + PGA1_c02440 0.26 +1.8 221,459 + PGA1_c02440 0.26 +1.9 221,459 + PGA1_c02440 0.26 +1.5 221,520 + PGA1_c02440 0.29 +1.5 221,528 - PGA1_c02440 0.29 +1.6 221,635 + PGA1_c02440 0.34 +1.4 221,635 + PGA1_c02440 0.34 +1.6 221,643 - PGA1_c02440 0.34 +1.1 221,643 - PGA1_c02440 0.34 +1.4 221,654 - PGA1_c02440 0.34 +2.4 221,690 + PGA1_c02440 0.36 +1.4 221,698 - PGA1_c02440 0.36 +2.1 221,708 - PGA1_c02440 0.37 +2.1 221,848 + PGA1_c02440 0.43 +0.9 221,945 - PGA1_c02440 0.47 +1.9 221,955 - PGA1_c02440 0.47 +1.3 221,973 - PGA1_c02440 0.48 +1.3 222,004 + PGA1_c02440 0.49 +1.3 222,007 - PGA1_c02440 0.49 +1.1 222,007 - PGA1_c02440 0.49 +1.0 222,052 - PGA1_c02440 0.51 +1.4 222,120 - PGA1_c02440 0.54 +1.4 222,122 - PGA1_c02440 0.54 +2.5 222,154 + PGA1_c02440 0.55 +1.9 222,260 - PGA1_c02440 0.60 +1.8 222,261 + PGA1_c02440 0.60 +1.8 222,269 - PGA1_c02440 0.60 +2.2 222,319 + PGA1_c02440 0.62 +1.5 222,327 - PGA1_c02440 0.63 +2.4 222,387 + PGA1_c02440 0.65 +1.0 222,390 - PGA1_c02440 0.65 +1.5 222,395 - PGA1_c02440 0.66 +1.4 222,395 - PGA1_c02440 0.66 +1.0 222,401 - PGA1_c02440 0.66 +1.3 222,408 + PGA1_c02440 0.66 +2.2 222,416 + PGA1_c02440 0.66 +1.6 222,416 + PGA1_c02440 0.66 +1.9 222,416 - PGA1_c02440 0.66 +1.4 222,416 - PGA1_c02440 0.66 +1.5 222,419 + PGA1_c02440 0.67 +1.0 222,422 - PGA1_c02440 0.67 +0.9 222,424 - PGA1_c02440 0.67 +1.8 222,461 + PGA1_c02440 0.68 +1.3 222,461 + PGA1_c02440 0.68 +1.5 222,532 + PGA1_c02440 0.71 +1.5 222,598 - PGA1_c02440 0.74 +1.6 222,598 - PGA1_c02440 0.74 +1.7 222,611 + PGA1_c02440 0.75 +0.1 222,704 - PGA1_c02440 0.79 +2.1 222,790 - PGA1_c02440 0.82 +2.2 222,819 + PGA1_c02440 0.84 +1.7 222,830 + PGA1_c02440 0.84 +2.2 222,897 + PGA1_c02440 0.87 +1.2 222,958 + PGA1_c02440 0.89 +1.9 222,966 - PGA1_c02440 0.90 +1.1 222,966 - PGA1_c02440 0.90 +1.6 223,030 - +1.8 223,062 - +1.8 223,094 - +1.2 223,094 - +2.3 223,125 - +1.0 223,184 - +1.6 223,186 + +1.5 223,203 + +1.5 223,254 + +1.7 223,267 - +1.4 223,312 + +1.9 223,315 - +2.0 223,341 + PGA1_c02450 0.10 +1.7 223,354 - PGA1_c02450 0.11 +1.9 223,356 + PGA1_c02450 0.11 +1.0 223,356 + PGA1_c02450 0.11 +1.2 223,356 + PGA1_c02450 0.11 +1.4 223,356 + PGA1_c02450 0.11 +1.2 223,356 + PGA1_c02450 0.11 +2.0 223,356 + PGA1_c02450 0.11 -0.0 223,356 + PGA1_c02450 0.11 +1.9 223,356 + PGA1_c02450 0.11 +1.6 223,356 + PGA1_c02450 0.11 +1.7 223,364 - PGA1_c02450 0.12 +1.7 223,364 - PGA1_c02450 0.12 +3.0 223,364 - PGA1_c02450 0.12 +2.5 223,364 - PGA1_c02450 0.12 +1.3 223,364 - PGA1_c02450 0.12 +2.6 223,364 - PGA1_c02450 0.12 +1.9 223,410 - PGA1_c02450 0.16 +1.8 223,410 - PGA1_c02450 0.16 +2.2 223,444 - PGA1_c02450 0.18 +2.1 223,499 + PGA1_c02450 0.22 +1.3 223,569 - PGA1_c02450 0.28 +1.1 223,579 + PGA1_c02450 0.28 +1.3 223,587 - PGA1_c02450 0.29 +1.0 223,605 - PGA1_c02450 0.30 +2.3 223,606 + PGA1_c02450 0.30 +1.0 223,634 + PGA1_c02450 0.33 -0.2 223,640 - PGA1_c02450 0.33 +1.0 223,670 - PGA1_c02450 0.35 +0.9 223,670 - PGA1_c02450 0.35 +2.0 223,672 + PGA1_c02450 0.35 +1.4 223,672 + PGA1_c02450 0.35 +2.0 223,680 - PGA1_c02450 0.36 +1.6 223,680 - PGA1_c02450 0.36 +0.8 223,680 - PGA1_c02450 0.36 +0.5 223,700 + PGA1_c02450 0.38 +1.1 223,742 + PGA1_c02450 0.41 +1.3 223,747 + PGA1_c02450 0.41 +1.3 223,843 - PGA1_c02450 0.48 +2.4 223,872 + PGA1_c02450 0.51 +0.8 223,873 - PGA1_c02450 0.51 +1.2 223,892 + PGA1_c02450 0.52 +1.6 223,900 - PGA1_c02450 0.53 +2.0 223,924 - PGA1_c02450 0.54 +1.4 223,962 - PGA1_c02450 0.57 +1.9 223,962 - PGA1_c02450 0.57 +1.4 223,962 - PGA1_c02450 0.57 +1.9 224,002 + PGA1_c02450 0.60 +1.3 224,002 + PGA1_c02450 0.60 +1.6 224,002 + PGA1_c02450 0.60 +2.0 224,002 + PGA1_c02450 0.60 +2.0 224,010 - PGA1_c02450 0.61 +1.4 224,010 - PGA1_c02450 0.61 +1.6 224,010 - PGA1_c02450 0.61 +0.2 224,010 - PGA1_c02450 0.61 +0.4 224,010 - PGA1_c02450 0.61 +1.5 224,010 - PGA1_c02450 0.61 +1.4 224,019 + PGA1_c02450 0.62 +1.4 224,027 - PGA1_c02450 0.62 +1.3 224,050 + PGA1_c02450 0.64 +1.4
Or see this region's nucleotide sequence