Experiment: m.b. Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c17480 and PGA1_c17490 are separated by 241 nucleotides PGA1_c17490 and PGA1_c17500 are separated by 87 nucleotides
PGA1_c17480: PGA1_c17480 - hemolysin-type calcium-binding repeat-containing protein, at 1,831,915 to 1,833,159
_c17480
PGA1_c17490: PGA1_c17490 - hemolysin-type calcium-binding repeat-containing protein, at 1,833,401 to 1,834,897
_c17490
PGA1_c17500: PGA1_c17500 - Protein of unknown function (DUF3179)., at 1,834,985 to 1,835,992
_c17500
Position (kb)
1833
1834
1835 Strain fitness (log2 ratio)
-2
-1
0
1 at 1832.498 kb on + strand, within PGA1_c17480 at 1832.638 kb on + strand, within PGA1_c17480 at 1832.646 kb on - strand, within PGA1_c17480 at 1832.648 kb on + strand, within PGA1_c17480 at 1832.765 kb on - strand, within PGA1_c17480 at 1832.804 kb on - strand, within PGA1_c17480 at 1832.838 kb on + strand, within PGA1_c17480 at 1832.846 kb on - strand, within PGA1_c17480 at 1833.063 kb on + strand at 1833.073 kb on + strand at 1833.106 kb on - strand at 1833.137 kb on - strand at 1833.153 kb on + strand at 1833.244 kb on + strand at 1833.333 kb on - strand at 1833.333 kb on - strand at 1833.379 kb on + strand at 1833.392 kb on + strand at 1833.392 kb on + strand at 1833.392 kb on + strand at 1833.392 kb on + strand at 1833.405 kb on - strand at 1833.563 kb on - strand, within PGA1_c17490 at 1833.563 kb on - strand, within PGA1_c17490 at 1833.636 kb on - strand, within PGA1_c17490 at 1833.644 kb on + strand, within PGA1_c17490 at 1833.645 kb on + strand, within PGA1_c17490 at 1833.645 kb on + strand, within PGA1_c17490 at 1833.645 kb on + strand, within PGA1_c17490 at 1833.645 kb on + strand, within PGA1_c17490 at 1833.650 kb on + strand, within PGA1_c17490 at 1833.825 kb on + strand, within PGA1_c17490 at 1833.846 kb on - strand, within PGA1_c17490 at 1833.854 kb on + strand, within PGA1_c17490 at 1833.854 kb on + strand, within PGA1_c17490 at 1833.862 kb on - strand, within PGA1_c17490 at 1833.891 kb on + strand, within PGA1_c17490 at 1833.906 kb on + strand, within PGA1_c17490 at 1833.914 kb on - strand, within PGA1_c17490 at 1833.914 kb on - strand, within PGA1_c17490 at 1833.914 kb on - strand, within PGA1_c17490 at 1833.992 kb on + strand, within PGA1_c17490 at 1834.178 kb on - strand, within PGA1_c17490 at 1834.296 kb on + strand, within PGA1_c17490 at 1834.498 kb on + strand, within PGA1_c17490 at 1834.501 kb on - strand, within PGA1_c17490 at 1834.646 kb on + strand, within PGA1_c17490 at 1834.650 kb on - strand, within PGA1_c17490 at 1834.796 kb on - strand at 1834.858 kb on - strand at 1834.870 kb on + strand at 1834.899 kb on - strand at 1834.914 kb on + strand at 1835.054 kb on - strand at 1835.059 kb on - strand at 1835.059 kb on - strand at 1835.059 kb on - strand at 1835.208 kb on + strand, within PGA1_c17500 at 1835.263 kb on - strand, within PGA1_c17500 at 1835.301 kb on - strand, within PGA1_c17500 at 1835.322 kb on - strand, within PGA1_c17500 at 1835.463 kb on + strand, within PGA1_c17500 at 1835.520 kb on - strand, within PGA1_c17500 at 1835.636 kb on - strand, within PGA1_c17500 at 1835.659 kb on + strand, within PGA1_c17500 at 1835.672 kb on - strand, within PGA1_c17500 at 1835.731 kb on + strand, within PGA1_c17500 at 1835.739 kb on + strand, within PGA1_c17500 at 1835.739 kb on + strand, within PGA1_c17500 at 1835.836 kb on - strand, within PGA1_c17500 at 1835.892 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Spectinomycin 0.00625 mg/ml remove 1,832,498 + PGA1_c17480 0.47 -0.6 1,832,638 + PGA1_c17480 0.58 -0.7 1,832,646 - PGA1_c17480 0.59 -0.1 1,832,648 + PGA1_c17480 0.59 +0.5 1,832,765 - PGA1_c17480 0.68 +0.6 1,832,804 - PGA1_c17480 0.71 -0.2 1,832,838 + PGA1_c17480 0.74 +0.0 1,832,846 - PGA1_c17480 0.75 -0.2 1,833,063 + -0.4 1,833,073 + +0.7 1,833,106 - +1.0 1,833,137 - +1.3 1,833,153 + -0.5 1,833,244 + +0.3 1,833,333 - +0.9 1,833,333 - +1.3 1,833,379 + +0.1 1,833,392 + +0.2 1,833,392 + +0.7 1,833,392 + -0.1 1,833,392 + +0.8 1,833,405 - -0.6 1,833,563 - PGA1_c17490 0.11 -0.6 1,833,563 - PGA1_c17490 0.11 +0.7 1,833,636 - PGA1_c17490 0.16 +0.6 1,833,644 + PGA1_c17490 0.16 -1.3 1,833,645 + PGA1_c17490 0.16 +0.6 1,833,645 + PGA1_c17490 0.16 +0.5 1,833,645 + PGA1_c17490 0.16 +1.1 1,833,645 + PGA1_c17490 0.16 -1.0 1,833,650 + PGA1_c17490 0.17 -1.8 1,833,825 + PGA1_c17490 0.28 -0.1 1,833,846 - PGA1_c17490 0.30 -0.2 1,833,854 + PGA1_c17490 0.30 +0.9 1,833,854 + PGA1_c17490 0.30 +0.2 1,833,862 - PGA1_c17490 0.31 +0.9 1,833,891 + PGA1_c17490 0.33 +0.5 1,833,906 + PGA1_c17490 0.34 +0.7 1,833,914 - PGA1_c17490 0.34 +1.0 1,833,914 - PGA1_c17490 0.34 -0.5 1,833,914 - PGA1_c17490 0.34 +0.1 1,833,992 + PGA1_c17490 0.39 +0.7 1,834,178 - PGA1_c17490 0.52 -0.1 1,834,296 + PGA1_c17490 0.60 +0.2 1,834,498 + PGA1_c17490 0.73 +0.7 1,834,501 - PGA1_c17490 0.73 +0.4 1,834,646 + PGA1_c17490 0.83 +0.1 1,834,650 - PGA1_c17490 0.83 +0.6 1,834,796 - +1.3 1,834,858 - -0.4 1,834,870 + -2.3 1,834,899 - +0.6 1,834,914 + +0.7 1,835,054 - +0.4 1,835,059 - +0.2 1,835,059 - +1.0 1,835,059 - +0.8 1,835,208 + PGA1_c17500 0.22 +0.1 1,835,263 - PGA1_c17500 0.28 +0.2 1,835,301 - PGA1_c17500 0.31 +0.4 1,835,322 - PGA1_c17500 0.33 -0.5 1,835,463 + PGA1_c17500 0.47 +0.5 1,835,520 - PGA1_c17500 0.53 -0.3 1,835,636 - PGA1_c17500 0.65 +1.2 1,835,659 + PGA1_c17500 0.67 -0.7 1,835,672 - PGA1_c17500 0.68 -0.2 1,835,731 + PGA1_c17500 0.74 +0.3 1,835,739 + PGA1_c17500 0.75 -0.3 1,835,739 + PGA1_c17500 0.75 +0.3 1,835,836 - PGA1_c17500 0.84 -0.7 1,835,892 + -0.6
Or see this region's nucleotide sequence