Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16225 and BWI76_RS16230 are separated by 36 nucleotides BWI76_RS16230 and BWI76_RS16235 overlap by 4 nucleotides BWI76_RS16235 and BWI76_RS16240 are separated by 990 nucleotides
BWI76_RS16225: BWI76_RS16225 - arginine:ornithine antiporter, at 3,365,468 to 3,366,850
_RS16225
BWI76_RS16230: BWI76_RS16230 - dihydromonapterin reductase, at 3,366,887 to 3,367,612
_RS16230
BWI76_RS16235: BWI76_RS16235 - membrane protein, at 3,367,609 to 3,367,944
_RS16235
BWI76_RS16240: BWI76_RS16240 - anti-adapter protein IraM, at 3,368,935 to 3,369,282
_RS16240
Position (kb)
3367
3368 Strain fitness (log2 ratio)
-1
0
1 at 3367.111 kb on + strand, within BWI76_RS16230 at 3367.342 kb on - strand, within BWI76_RS16230 at 3367.527 kb on + strand at 3367.557 kb on - strand at 3367.647 kb on + strand, within BWI76_RS16235 at 3367.654 kb on + strand, within BWI76_RS16235 at 3367.816 kb on - strand, within BWI76_RS16235 at 3368.773 kb on - strand at 3368.900 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,367,111 + BWI76_RS16230 0.31 -0.2 3,367,342 - BWI76_RS16230 0.63 +0.1 3,367,527 + +1.0 3,367,557 - -0.2 3,367,647 + BWI76_RS16235 0.11 +0.6 3,367,654 + BWI76_RS16235 0.13 +1.3 3,367,816 - BWI76_RS16235 0.62 -0.2 3,368,773 - -0.4 3,368,900 - -0.4
Or see this region's nucleotide sequence