Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09145

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09135 and BWI76_RS09140 are separated by 2 nucleotidesBWI76_RS09140 and BWI76_RS09145 are separated by 257 nucleotidesBWI76_RS09145 and BWI76_RS09150 are separated by 28 nucleotidesBWI76_RS09150 and BWI76_RS09155 are separated by 125 nucleotides BWI76_RS09135: BWI76_RS09135 - oxidoreductase, at 1,929,961 to 1,931,073 _RS09135 BWI76_RS09140: BWI76_RS09140 - glutathione S-transferase, at 1,931,076 to 1,931,702 _RS09140 BWI76_RS09145: BWI76_RS09145 - serine-type D-Ala-D-Ala carboxypeptidase, at 1,931,960 to 1,933,162 _RS09145 BWI76_RS09150: BWI76_RS09150 - DNA-binding transcriptional repressor DeoR, at 1,933,191 to 1,933,949 _RS09150 BWI76_RS09155: BWI76_RS09155 - undecaprenyl-diphosphatase, at 1,934,075 to 1,934,668 _RS09155 Position (kb) 1931 1932 1933 1934Strain fitness (log2 ratio) -1 0 1at 1931.042 kb on - strandat 1931.229 kb on + strand, within BWI76_RS09140at 1931.268 kb on + strand, within BWI76_RS09140at 1931.352 kb on - strand, within BWI76_RS09140at 1931.352 kb on - strand, within BWI76_RS09140at 1931.400 kb on - strand, within BWI76_RS09140at 1931.881 kb on + strandat 1932.218 kb on - strand, within BWI76_RS09145at 1932.451 kb on + strand, within BWI76_RS09145at 1932.507 kb on - strand, within BWI76_RS09145at 1933.337 kb on + strand, within BWI76_RS09150at 1933.391 kb on + strand, within BWI76_RS09150at 1933.393 kb on - strand, within BWI76_RS09150at 1933.765 kb on - strand, within BWI76_RS09150at 1933.892 kb on - strandat 1933.896 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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1,931,042 - +0.2
1,931,229 + BWI76_RS09140 0.24 +0.1
1,931,268 + BWI76_RS09140 0.31 -0.1
1,931,352 - BWI76_RS09140 0.44 +0.0
1,931,352 - BWI76_RS09140 0.44 -1.4
1,931,400 - BWI76_RS09140 0.52 +0.0
1,931,881 + +0.3
1,932,218 - BWI76_RS09145 0.21 +0.8
1,932,451 + BWI76_RS09145 0.41 +0.5
1,932,507 - BWI76_RS09145 0.45 -0.1
1,933,337 + BWI76_RS09150 0.19 +0.6
1,933,391 + BWI76_RS09150 0.26 -0.1
1,933,393 - BWI76_RS09150 0.27 +0.0
1,933,765 - BWI76_RS09150 0.76 -0.5
1,933,892 - -0.8
1,933,896 + -0.4

Or see this region's nucleotide sequence