Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04215

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04205 and BWI76_RS04210 are separated by 209 nucleotidesBWI76_RS04210 and BWI76_RS04215 are separated by 12 nucleotidesBWI76_RS04215 and BWI76_RS04220 overlap by 1 nucleotides BWI76_RS04205: BWI76_RS04205 - right oriC-binding transcriptional activator, at 864,977 to 865,846 _RS04205 BWI76_RS04210: BWI76_RS04210 - hypothetical protein, at 866,056 to 866,529 _RS04210 BWI76_RS04215: BWI76_RS04215 - DNA-binding response regulator, at 866,542 to 867,231 _RS04215 BWI76_RS04220: BWI76_RS04220 - two-component sensor histidine kinase, at 867,231 to 868,655 _RS04220 Position (kb) 866 867 868Strain fitness (log2 ratio) -2 -1 0 1 2 3at 865.551 kb on - strand, within BWI76_RS04205at 865.653 kb on - strand, within BWI76_RS04205at 865.759 kb on - strand, within BWI76_RS04205at 865.767 kb on + strandat 865.800 kb on + strandat 865.809 kb on + strandat 865.811 kb on + strandat 865.811 kb on + strandat 865.811 kb on + strandat 865.811 kb on + strandat 865.811 kb on + strandat 865.811 kb on + strandat 865.811 kb on + strandat 865.819 kb on - strandat 865.819 kb on - strandat 865.819 kb on - strandat 865.819 kb on - strandat 865.819 kb on - strandat 865.859 kb on + strandat 865.859 kb on + strandat 865.859 kb on + strandat 865.866 kb on + strandat 866.247 kb on + strand, within BWI76_RS04210at 866.258 kb on + strand, within BWI76_RS04210at 866.279 kb on + strand, within BWI76_RS04210at 866.279 kb on + strand, within BWI76_RS04210at 866.287 kb on - strand, within BWI76_RS04210at 866.551 kb on + strandat 866.598 kb on + strandat 866.606 kb on - strandat 866.606 kb on - strandat 866.734 kb on - strand, within BWI76_RS04215at 866.786 kb on - strand, within BWI76_RS04215at 866.786 kb on - strand, within BWI76_RS04215at 866.803 kb on - strand, within BWI76_RS04215at 867.088 kb on + strand, within BWI76_RS04215at 867.103 kb on + strand, within BWI76_RS04215at 867.132 kb on - strand, within BWI76_RS04215at 867.215 kb on + strandat 867.215 kb on + strandat 867.356 kb on + strandat 867.533 kb on + strand, within BWI76_RS04220at 867.541 kb on - strand, within BWI76_RS04220at 867.541 kb on - strand, within BWI76_RS04220at 867.707 kb on + strand, within BWI76_RS04220at 867.714 kb on + strand, within BWI76_RS04220at 867.775 kb on + strand, within BWI76_RS04220at 867.928 kb on + strand, within BWI76_RS04220at 867.928 kb on + strand, within BWI76_RS04220at 867.936 kb on - strand, within BWI76_RS04220at 867.936 kb on - strand, within BWI76_RS04220at 867.936 kb on - strand, within BWI76_RS04220at 867.948 kb on + strand, within BWI76_RS04220at 867.970 kb on + strand, within BWI76_RS04220at 867.970 kb on + strand, within BWI76_RS04220at 868.189 kb on + strand, within BWI76_RS04220

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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865,551 - BWI76_RS04205 0.66 -0.1
865,653 - BWI76_RS04205 0.78 +0.4
865,759 - BWI76_RS04205 0.90 +0.0
865,767 + -0.2
865,800 + -0.0
865,809 + -0.2
865,811 + +0.3
865,811 + +0.3
865,811 + +0.3
865,811 + -1.3
865,811 + +0.3
865,811 + -0.7
865,811 + -2.0
865,819 - +0.6
865,819 - +0.3
865,819 - -0.1
865,819 - +0.3
865,819 - +0.5
865,859 + -0.4
865,859 + -0.6
865,859 + +0.8
865,866 + -0.9
866,247 + BWI76_RS04210 0.40 +3.2
866,258 + BWI76_RS04210 0.43 -0.0
866,279 + BWI76_RS04210 0.47 +0.0
866,279 + BWI76_RS04210 0.47 -0.1
866,287 - BWI76_RS04210 0.49 -0.6
866,551 + +1.0
866,598 + +0.8
866,606 - +0.7
866,606 - -0.4
866,734 - BWI76_RS04215 0.28 +0.6
866,786 - BWI76_RS04215 0.35 +1.1
866,786 - BWI76_RS04215 0.35 +0.5
866,803 - BWI76_RS04215 0.38 +0.5
867,088 + BWI76_RS04215 0.79 -0.3
867,103 + BWI76_RS04215 0.81 -0.2
867,132 - BWI76_RS04215 0.86 +2.6
867,215 + +0.1
867,215 + +0.2
867,356 + -0.3
867,533 + BWI76_RS04220 0.21 +0.5
867,541 - BWI76_RS04220 0.22 +0.1
867,541 - BWI76_RS04220 0.22 +0.1
867,707 + BWI76_RS04220 0.33 +0.7
867,714 + BWI76_RS04220 0.34 -0.1
867,775 + BWI76_RS04220 0.38 -1.6
867,928 + BWI76_RS04220 0.49 +0.7
867,928 + BWI76_RS04220 0.49 +0.5
867,936 - BWI76_RS04220 0.49 +0.1
867,936 - BWI76_RS04220 0.49 +0.5
867,936 - BWI76_RS04220 0.49 +0.6
867,948 + BWI76_RS04220 0.50 -0.2
867,970 + BWI76_RS04220 0.52 -0.2
867,970 + BWI76_RS04220 0.52 +1.0
868,189 + BWI76_RS04220 0.67 +0.0

Or see this region's nucleotide sequence