Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02205

Experiment: LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02195 and BWI76_RS02200 overlap by 4 nucleotidesBWI76_RS02200 and BWI76_RS02205 are separated by 196 nucleotidesBWI76_RS02205 and BWI76_RS02210 overlap by 4 nucleotidesBWI76_RS02210 and BWI76_RS02215 overlap by 8 nucleotides BWI76_RS02195: BWI76_RS02195 - alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, at 454,745 to 455,881 _RS02195 BWI76_RS02200: BWI76_RS02200 - phosphonate C-P lyase system protein PhnL, at 455,878 to 456,564 _RS02200 BWI76_RS02205: BWI76_RS02205 - phosphonate C-P lyase system protein PhnK, at 456,761 to 457,519 _RS02205 BWI76_RS02210: BWI76_RS02210 - carbon-phosphorus lyase complex subunit PhnJ, at 457,516 to 458,364 _RS02210 BWI76_RS02215: BWI76_RS02215 - carbon-phosphorus lyase complex subunit PhnI, at 458,357 to 459,421 _RS02215 Position (kb) 456 457 458Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 455.860 kb on + strandat 455.860 kb on + strandat 455.868 kb on - strandat 455.904 kb on + strandat 456.015 kb on - strand, within BWI76_RS02200at 456.016 kb on + strand, within BWI76_RS02200at 456.268 kb on - strand, within BWI76_RS02200at 456.309 kb on - strand, within BWI76_RS02200at 456.316 kb on - strand, within BWI76_RS02200at 456.316 kb on - strand, within BWI76_RS02200at 456.326 kb on - strand, within BWI76_RS02200at 456.439 kb on + strand, within BWI76_RS02200at 456.648 kb on - strandat 456.716 kb on + strandat 456.724 kb on - strandat 456.747 kb on + strandat 456.795 kb on + strandat 456.795 kb on + strandat 456.803 kb on - strandat 456.931 kb on + strand, within BWI76_RS02205at 457.034 kb on + strand, within BWI76_RS02205at 457.064 kb on - strand, within BWI76_RS02205at 457.074 kb on - strand, within BWI76_RS02205at 457.164 kb on + strand, within BWI76_RS02205at 457.164 kb on + strand, within BWI76_RS02205at 457.194 kb on - strand, within BWI76_RS02205at 457.436 kb on + strand, within BWI76_RS02205at 457.473 kb on + strandat 457.637 kb on + strand, within BWI76_RS02210at 457.654 kb on + strand, within BWI76_RS02210at 457.701 kb on + strandat 457.718 kb on + strand, within BWI76_RS02210at 457.752 kb on + strand, within BWI76_RS02210at 457.760 kb on - strand, within BWI76_RS02210at 457.826 kb on + strand, within BWI76_RS02210at 457.854 kb on - strand, within BWI76_RS02210at 457.854 kb on - strand, within BWI76_RS02210at 457.854 kb on - strand, within BWI76_RS02210at 457.925 kb on - strand, within BWI76_RS02210at 457.974 kb on + strand, within BWI76_RS02210at 458.223 kb on - strand, within BWI76_RS02210at 458.385 kb on + strandat 458.385 kb on + strandat 458.393 kb on - strandat 458.393 kb on - strandat 458.393 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB
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455,860 + -0.3
455,860 + +0.0
455,868 - +0.6
455,904 + +0.5
456,015 - BWI76_RS02200 0.20 -1.3
456,016 + BWI76_RS02200 0.20 -0.1
456,268 - BWI76_RS02200 0.57 +1.2
456,309 - BWI76_RS02200 0.63 +0.6
456,316 - BWI76_RS02200 0.64 -1.1
456,316 - BWI76_RS02200 0.64 -0.1
456,326 - BWI76_RS02200 0.65 +0.4
456,439 + BWI76_RS02200 0.82 -1.8
456,648 - +0.3
456,716 + -0.1
456,724 - -0.0
456,747 + +0.4
456,795 + +0.4
456,795 + +0.9
456,803 - +0.1
456,931 + BWI76_RS02205 0.22 -0.5
457,034 + BWI76_RS02205 0.36 -0.3
457,064 - BWI76_RS02205 0.40 +0.7
457,074 - BWI76_RS02205 0.41 -0.1
457,164 + BWI76_RS02205 0.53 +0.0
457,164 + BWI76_RS02205 0.53 -0.5
457,194 - BWI76_RS02205 0.57 +2.3
457,436 + BWI76_RS02205 0.89 +0.3
457,473 + +0.5
457,637 + BWI76_RS02210 0.14 +0.8
457,654 + BWI76_RS02210 0.16 -0.5
457,701 + -0.0
457,718 + BWI76_RS02210 0.24 +0.1
457,752 + BWI76_RS02210 0.28 +2.4
457,760 - BWI76_RS02210 0.29 -0.2
457,826 + BWI76_RS02210 0.37 +0.3
457,854 - BWI76_RS02210 0.40 -1.3
457,854 - BWI76_RS02210 0.40 +0.2
457,854 - BWI76_RS02210 0.40 +1.2
457,925 - BWI76_RS02210 0.48 -2.9
457,974 + BWI76_RS02210 0.54 -0.4
458,223 - BWI76_RS02210 0.83 +0.1
458,385 + +0.8
458,385 + -0.9
458,393 - +0.9
458,393 - +0.3
458,393 - +0.3

Or see this region's nucleotide sequence