Experiment: m.b. Zinc 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_322 and HP15_323 are separated by 22 nucleotides HP15_323 and HP15_324 overlap by 4 nucleotides HP15_324 and HP15_325 are separated by 59 nucleotides HP15_325 and HP15_326 overlap by 4 nucleotides
HP15_322: HP15_322 - glucose-6-phosphate isomerase, at 318,318 to 319,979
_322
HP15_323: HP15_323 - pantothenate synthetase, at 320,002 to 320,850
_323
HP15_324: HP15_324 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 320,847 to 321,641
_324
HP15_325: HP15_325 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 321,701 to 322,198
_325
HP15_326: HP15_326 - polyA polymerase, at 322,195 to 323,526
_326
Position (kb) 320
321
322 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 319.985 kb on - strand at 321.670 kb on - strand at 322.261 kb on - strand at 322.273 kb on - strand at 322.293 kb on - strand at 322.382 kb on + strand, within HP15_326 at 322.562 kb on - strand, within HP15_326 at 322.573 kb on - strand, within HP15_326 at 322.577 kb on - strand, within HP15_326 at 322.604 kb on + strand, within HP15_326
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Zinc 0.5 mM remove 319,985 - -0.5 321,670 - +0.9 322,261 - -1.4 322,273 - +0.6 322,293 - +0.9 322,382 + HP15_326 0.14 -1.6 322,562 - HP15_326 0.28 -1.6 322,573 - HP15_326 0.28 -1.2 322,577 - HP15_326 0.29 -3.5 322,604 + HP15_326 0.31 -1.8
Or see this region's nucleotide sequence