Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flhB and fliR are separated by 1 nucleotides fliR and Sama_2286 overlap by 1 nucleotides Sama_2286 and fliP are separated by 5 nucleotides
Sama_2284: flhB - flagellar biosynthesis protein FlhB (RefSeq), at 2,751,027 to 2,752,160
flhB
Sama_2285: fliR - flagellar biosynthesis protein FliR (RefSeq), at 2,752,162 to 2,752,950
fliR
Sama_2286: Sama_2286 - flagellar biosynthetic protein FliQ (RefSeq), at 2,752,950 to 2,753,219
_2286
Sama_2287: fliP - flagellar biosynthesis protein FliP (RefSeq), at 2,753,225 to 2,753,974
fliP
Position (kb)
2752
2753 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2751.182 kb on + strand, within flhB at 2751.182 kb on + strand, within flhB at 2751.182 kb on + strand, within flhB at 2751.260 kb on + strand, within flhB at 2751.323 kb on - strand, within flhB at 2751.323 kb on - strand, within flhB at 2751.361 kb on - strand, within flhB at 2751.361 kb on - strand, within flhB at 2751.592 kb on - strand, within flhB at 2751.636 kb on + strand, within flhB at 2751.756 kb on - strand, within flhB at 2751.831 kb on + strand, within flhB at 2751.831 kb on + strand, within flhB at 2751.831 kb on + strand, within flhB at 2751.832 kb on - strand, within flhB at 2751.832 kb on - strand, within flhB at 2751.832 kb on - strand, within flhB at 2751.832 kb on - strand, within flhB at 2751.832 kb on - strand, within flhB at 2751.921 kb on - strand, within flhB at 2751.921 kb on - strand, within flhB at 2751.921 kb on - strand, within flhB at 2752.130 kb on + strand at 2752.146 kb on - strand at 2752.282 kb on - strand, within fliR at 2752.314 kb on + strand, within fliR at 2752.315 kb on - strand, within fliR at 2752.315 kb on - strand, within fliR at 2752.319 kb on - strand, within fliR at 2752.319 kb on - strand, within fliR at 2752.319 kb on - strand, within fliR at 2752.418 kb on + strand, within fliR at 2752.418 kb on + strand, within fliR at 2752.419 kb on - strand, within fliR at 2752.419 kb on - strand, within fliR at 2752.419 kb on - strand, within fliR at 2752.419 kb on - strand, within fliR at 2752.419 kb on - strand, within fliR at 2752.446 kb on - strand, within fliR at 2752.446 kb on - strand, within fliR at 2752.446 kb on - strand, within fliR at 2752.446 kb on - strand, within fliR at 2752.450 kb on - strand, within fliR at 2752.471 kb on - strand, within fliR at 2752.471 kb on - strand, within fliR at 2752.485 kb on + strand, within fliR at 2752.780 kb on + strand, within fliR at 2752.781 kb on - strand, within fliR at 2752.845 kb on + strand, within fliR at 2752.845 kb on + strand, within fliR at 2752.846 kb on - strand, within fliR at 2752.846 kb on - strand, within fliR at 2752.872 kb on + strand at 2752.873 kb on - strand at 2752.873 kb on - strand at 2752.905 kb on + strand at 2752.905 kb on + strand at 2752.905 kb on + strand at 2752.905 kb on + strand at 2752.905 kb on + strand at 2752.906 kb on - strand at 2752.906 kb on - strand at 2752.908 kb on - strand at 2752.908 kb on - strand at 2752.918 kb on - strand at 2752.918 kb on - strand at 2752.918 kb on - strand at 2752.960 kb on - strand at 2753.147 kb on + strand, within Sama_2286 at 2753.148 kb on - strand, within Sama_2286 at 2753.148 kb on - strand, within Sama_2286 at 2753.148 kb on - strand, within Sama_2286 at 2753.166 kb on + strand, within Sama_2286 at 2753.166 kb on + strand, within Sama_2286 at 2753.167 kb on - strand, within Sama_2286 at 2753.167 kb on - strand, within Sama_2286 at 2753.167 kb on - strand, within Sama_2286 at 2753.167 kb on - strand, within Sama_2286 at 2753.167 kb on - strand, within Sama_2286 at 2753.169 kb on + strand, within Sama_2286 at 2753.195 kb on + strand at 2753.195 kb on + strand at 2753.196 kb on - strand at 2753.196 kb on - strand at 2753.231 kb on - strand at 2753.401 kb on + strand, within fliP at 2753.402 kb on - strand, within fliP at 2753.453 kb on - strand, within fliP at 2753.453 kb on - strand, within fliP at 2753.551 kb on + strand, within fliP at 2753.551 kb on + strand, within fliP at 2753.551 kb on + strand, within fliP at 2753.552 kb on - strand, within fliP at 2753.552 kb on - strand, within fliP at 2753.552 kb on - strand, within fliP at 2753.552 kb on - strand, within fliP at 2753.552 kb on - strand, within fliP at 2753.552 kb on - strand, within fliP at 2753.552 kb on - strand, within fliP at 2753.573 kb on - strand, within fliP at 2753.614 kb on + strand, within fliP at 2753.615 kb on - strand, within fliP at 2753.615 kb on - strand, within fliP at 2753.615 kb on - strand, within fliP at 2753.616 kb on + strand, within fliP at 2753.617 kb on - strand, within fliP at 2753.617 kb on - strand, within fliP at 2753.617 kb on - strand, within fliP at 2753.617 kb on - strand, within fliP at 2753.678 kb on - strand, within fliP at 2753.759 kb on + strand, within fliP at 2753.759 kb on + strand, within fliP at 2753.760 kb on - strand, within fliP at 2753.760 kb on - strand, within fliP at 2753.788 kb on + strand, within fliP
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,751,182 + flhB Sama_2284 0.14 -0.5 2,751,182 + flhB Sama_2284 0.14 -2.1 2,751,182 + flhB Sama_2284 0.14 -2.5 2,751,260 + flhB Sama_2284 0.21 -0.1 2,751,323 - flhB Sama_2284 0.26 -0.9 2,751,323 - flhB Sama_2284 0.26 -1.5 2,751,361 - flhB Sama_2284 0.29 -0.5 2,751,361 - flhB Sama_2284 0.29 -1.4 2,751,592 - flhB Sama_2284 0.50 -3.1 2,751,636 + flhB Sama_2284 0.54 -2.4 2,751,756 - flhB Sama_2284 0.64 -1.5 2,751,831 + flhB Sama_2284 0.71 -1.1 2,751,831 + flhB Sama_2284 0.71 -2.2 2,751,831 + flhB Sama_2284 0.71 -2.0 2,751,832 - flhB Sama_2284 0.71 -0.5 2,751,832 - flhB Sama_2284 0.71 -3.1 2,751,832 - flhB Sama_2284 0.71 -1.9 2,751,832 - flhB Sama_2284 0.71 +0.5 2,751,832 - flhB Sama_2284 0.71 -1.7 2,751,921 - flhB Sama_2284 0.79 -1.5 2,751,921 - flhB Sama_2284 0.79 -2.5 2,751,921 - flhB Sama_2284 0.79 -0.9 2,752,130 + -0.9 2,752,146 - -3.8 2,752,282 - fliR Sama_2285 0.15 -1.4 2,752,314 + fliR Sama_2285 0.19 -1.9 2,752,315 - fliR Sama_2285 0.19 -1.1 2,752,315 - fliR Sama_2285 0.19 -1.5 2,752,319 - fliR Sama_2285 0.20 -3.2 2,752,319 - fliR Sama_2285 0.20 -1.7 2,752,319 - fliR Sama_2285 0.20 -0.1 2,752,418 + fliR Sama_2285 0.32 -2.4 2,752,418 + fliR Sama_2285 0.32 -0.1 2,752,419 - fliR Sama_2285 0.33 -3.6 2,752,419 - fliR Sama_2285 0.33 -2.5 2,752,419 - fliR Sama_2285 0.33 -1.5 2,752,419 - fliR Sama_2285 0.33 -0.9 2,752,419 - fliR Sama_2285 0.33 -2.9 2,752,446 - fliR Sama_2285 0.36 -0.5 2,752,446 - fliR Sama_2285 0.36 -2.1 2,752,446 - fliR Sama_2285 0.36 -0.5 2,752,446 - fliR Sama_2285 0.36 -2.6 2,752,450 - fliR Sama_2285 0.37 -1.5 2,752,471 - fliR Sama_2285 0.39 -0.5 2,752,471 - fliR Sama_2285 0.39 -0.5 2,752,485 + fliR Sama_2285 0.41 -1.7 2,752,780 + fliR Sama_2285 0.78 -3.8 2,752,781 - fliR Sama_2285 0.78 -1.4 2,752,845 + fliR Sama_2285 0.87 -1.1 2,752,845 + fliR Sama_2285 0.87 -0.5 2,752,846 - fliR Sama_2285 0.87 -2.9 2,752,846 - fliR Sama_2285 0.87 -2.2 2,752,872 + -0.1 2,752,873 - +0.5 2,752,873 - -1.4 2,752,905 + -1.1 2,752,905 + +1.5 2,752,905 + -0.5 2,752,905 + +0.5 2,752,905 + -0.9 2,752,906 - -2.9 2,752,906 - -0.1 2,752,908 - -0.9 2,752,908 - -0.9 2,752,918 - -1.9 2,752,918 - -2.7 2,752,918 - -2.0 2,752,960 - -1.7 2,753,147 + Sama_2286 0.73 -2.1 2,753,148 - Sama_2286 0.73 -3.6 2,753,148 - Sama_2286 0.73 -1.9 2,753,148 - Sama_2286 0.73 -1.5 2,753,166 + Sama_2286 0.80 -2.1 2,753,166 + Sama_2286 0.80 -1.4 2,753,167 - Sama_2286 0.80 -2.5 2,753,167 - Sama_2286 0.80 -2.1 2,753,167 - Sama_2286 0.80 -1.1 2,753,167 - Sama_2286 0.80 -0.9 2,753,167 - Sama_2286 0.80 -0.2 2,753,169 + Sama_2286 0.81 -1.4 2,753,195 + -1.4 2,753,195 + -0.1 2,753,196 - -2.4 2,753,196 - -1.5 2,753,231 - -1.1 2,753,401 + fliP Sama_2287 0.23 -2.8 2,753,402 - fliP Sama_2287 0.24 -2.1 2,753,453 - fliP Sama_2287 0.30 -2.2 2,753,453 - fliP Sama_2287 0.30 -2.5 2,753,551 + fliP Sama_2287 0.43 -0.5 2,753,551 + fliP Sama_2287 0.43 -0.5 2,753,551 + fliP Sama_2287 0.43 -0.1 2,753,552 - fliP Sama_2287 0.44 -0.1 2,753,552 - fliP Sama_2287 0.44 -2.4 2,753,552 - fliP Sama_2287 0.44 -0.9 2,753,552 - fliP Sama_2287 0.44 -1.7 2,753,552 - fliP Sama_2287 0.44 -2.4 2,753,552 - fliP Sama_2287 0.44 -3.1 2,753,552 - fliP Sama_2287 0.44 -1.4 2,753,573 - fliP Sama_2287 0.46 -1.4 2,753,614 + fliP Sama_2287 0.52 -0.5 2,753,615 - fliP Sama_2287 0.52 -2.5 2,753,615 - fliP Sama_2287 0.52 -3.2 2,753,615 - fliP Sama_2287 0.52 +0.5 2,753,616 + fliP Sama_2287 0.52 -1.5 2,753,617 - fliP Sama_2287 0.52 -1.1 2,753,617 - fliP Sama_2287 0.52 -1.1 2,753,617 - fliP Sama_2287 0.52 -2.0 2,753,617 - fliP Sama_2287 0.52 -2.5 2,753,678 - fliP Sama_2287 0.60 -0.5 2,753,759 + fliP Sama_2287 0.71 -0.5 2,753,759 + fliP Sama_2287 0.71 -1.5 2,753,760 - fliP Sama_2287 0.71 -2.5 2,753,760 - fliP Sama_2287 0.71 -3.6 2,753,788 + fliP Sama_2287 0.75 -2.4
Or see this region's nucleotide sequence