Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1351 and Sama_1352 are separated by 132 nucleotides Sama_1352 and Sama_1353 are separated by 71 nucleotides Sama_1353 and Sama_1354 are separated by 81 nucleotides Sama_1354 and Sama_1355 are separated by 330 nucleotides
Sama_1351: Sama_1351 - beta-lactamase class C-like protein (RefSeq), at 1,640,228 to 1,641,382
_1351
Sama_1352: Sama_1352 - hypothetical protein (RefSeq), at 1,641,515 to 1,641,769
_1352
Sama_1353: Sama_1353 - hypothetical protein (RefSeq), at 1,641,841 to 1,642,863
_1353
Sama_1354: Sama_1354 - hypothetical protein (RefSeq), at 1,642,945 to 1,643,427
_1354
Sama_1355: Sama_1355 - phage integrase family site specific recombinase (RefSeq), at 1,643,758 to 1,644,714
_1355
Position (kb)
1641
1642
1643 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 1640.933 kb on - strand, within Sama_1351 at 1640.945 kb on + strand, within Sama_1351 at 1640.946 kb on - strand, within Sama_1351 at 1640.946 kb on - strand, within Sama_1351 at 1640.946 kb on - strand, within Sama_1351 at 1641.038 kb on - strand, within Sama_1351 at 1641.150 kb on - strand, within Sama_1351 at 1641.199 kb on - strand, within Sama_1351 at 1641.359 kb on + strand at 1641.434 kb on - strand at 1641.479 kb on - strand at 1641.479 kb on - strand at 1641.534 kb on - strand at 1641.591 kb on + strand, within Sama_1352 at 1641.592 kb on - strand, within Sama_1352 at 1641.592 kb on - strand, within Sama_1352 at 1641.594 kb on - strand, within Sama_1352 at 1641.733 kb on + strand, within Sama_1352 at 1641.754 kb on + strand at 1641.842 kb on + strand at 1641.918 kb on + strand at 1641.919 kb on - strand at 1641.923 kb on - strand at 1641.923 kb on - strand at 1641.955 kb on + strand, within Sama_1353 at 1641.979 kb on + strand, within Sama_1353 at 1642.043 kb on - strand, within Sama_1353 at 1642.049 kb on - strand, within Sama_1353 at 1642.051 kb on + strand, within Sama_1353 at 1642.117 kb on + strand, within Sama_1353 at 1642.117 kb on + strand, within Sama_1353 at 1642.118 kb on - strand, within Sama_1353 at 1642.118 kb on - strand, within Sama_1353 at 1642.118 kb on - strand, within Sama_1353 at 1642.119 kb on + strand, within Sama_1353 at 1642.120 kb on - strand, within Sama_1353 at 1642.172 kb on - strand, within Sama_1353 at 1642.172 kb on - strand, within Sama_1353 at 1642.223 kb on - strand, within Sama_1353 at 1642.246 kb on + strand, within Sama_1353 at 1642.246 kb on + strand, within Sama_1353 at 1642.331 kb on - strand, within Sama_1353 at 1642.421 kb on - strand, within Sama_1353 at 1642.421 kb on - strand, within Sama_1353 at 1642.605 kb on + strand, within Sama_1353 at 1642.628 kb on - strand, within Sama_1353 at 1642.759 kb on + strand, within Sama_1353 at 1642.778 kb on - strand at 1642.865 kb on + strand at 1642.869 kb on - strand at 1642.869 kb on - strand at 1642.869 kb on - strand at 1642.946 kb on + strand at 1642.967 kb on + strand at 1642.968 kb on - strand at 1642.980 kb on - strand at 1642.980 kb on - strand at 1643.011 kb on + strand, within Sama_1354 at 1643.012 kb on - strand, within Sama_1354 at 1643.137 kb on - strand, within Sama_1354 at 1643.166 kb on + strand, within Sama_1354 at 1643.167 kb on - strand, within Sama_1354 at 1643.167 kb on - strand, within Sama_1354 at 1643.170 kb on + strand, within Sama_1354 at 1643.204 kb on + strand, within Sama_1354 at 1643.241 kb on + strand, within Sama_1354 at 1643.302 kb on + strand, within Sama_1354 at 1643.302 kb on + strand, within Sama_1354 at 1643.410 kb on + strand at 1643.431 kb on + strand at 1643.431 kb on + strand at 1643.432 kb on - strand at 1643.432 kb on - strand at 1643.432 kb on - strand at 1643.432 kb on - strand at 1643.435 kb on + strand at 1643.447 kb on + strand at 1643.455 kb on + strand at 1643.475 kb on - strand at 1643.548 kb on + strand at 1643.602 kb on - strand at 1643.796 kb on + strand at 1643.797 kb on - strand at 1643.797 kb on - strand at 1643.798 kb on + strand at 1643.798 kb on + strand at 1643.798 kb on + strand at 1643.798 kb on + strand at 1643.798 kb on + strand at 1643.799 kb on - strand at 1643.799 kb on - strand at 1643.799 kb on - strand at 1643.850 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,640,933 - Sama_1351 0.61 -0.4 1,640,945 + Sama_1351 0.62 -1.2 1,640,946 - Sama_1351 0.62 -2.6 1,640,946 - Sama_1351 0.62 -0.0 1,640,946 - Sama_1351 0.62 -0.9 1,641,038 - Sama_1351 0.70 +1.5 1,641,150 - Sama_1351 0.80 -0.4 1,641,199 - Sama_1351 0.84 -2.2 1,641,359 + +0.0 1,641,434 - -1.7 1,641,479 - -1.3 1,641,479 - -1.9 1,641,534 - +0.1 1,641,591 + Sama_1352 0.30 +3.7 1,641,592 - Sama_1352 0.30 -0.3 1,641,592 - Sama_1352 0.30 -2.0 1,641,594 - Sama_1352 0.31 +1.4 1,641,733 + Sama_1352 0.85 -1.2 1,641,754 + -1.7 1,641,842 + -0.9 1,641,918 + -1.9 1,641,919 - -0.4 1,641,923 - +5.1 1,641,923 - -2.7 1,641,955 + Sama_1353 0.11 +2.1 1,641,979 + Sama_1353 0.13 +0.1 1,642,043 - Sama_1353 0.20 -2.2 1,642,049 - Sama_1353 0.20 +0.4 1,642,051 + Sama_1353 0.21 -0.4 1,642,117 + Sama_1353 0.27 +0.7 1,642,117 + Sama_1353 0.27 -0.6 1,642,118 - Sama_1353 0.27 -1.7 1,642,118 - Sama_1353 0.27 +0.6 1,642,118 - Sama_1353 0.27 -2.3 1,642,119 + Sama_1353 0.27 +0.5 1,642,120 - Sama_1353 0.27 +0.2 1,642,172 - Sama_1353 0.32 -1.4 1,642,172 - Sama_1353 0.32 +0.6 1,642,223 - Sama_1353 0.37 -0.9 1,642,246 + Sama_1353 0.40 -1.8 1,642,246 + Sama_1353 0.40 -4.1 1,642,331 - Sama_1353 0.48 +0.6 1,642,421 - Sama_1353 0.57 +0.7 1,642,421 - Sama_1353 0.57 +0.9 1,642,605 + Sama_1353 0.75 -1.4 1,642,628 - Sama_1353 0.77 -1.8 1,642,759 + Sama_1353 0.90 +2.8 1,642,778 - +2.5 1,642,865 + -1.7 1,642,869 - +1.2 1,642,869 - -1.4 1,642,869 - -1.4 1,642,946 + +1.1 1,642,967 + +1.0 1,642,968 - -1.3 1,642,980 - -1.2 1,642,980 - +1.1 1,643,011 + Sama_1354 0.14 +2.9 1,643,012 - Sama_1354 0.14 +0.6 1,643,137 - Sama_1354 0.40 +0.7 1,643,166 + Sama_1354 0.46 +0.3 1,643,167 - Sama_1354 0.46 -1.3 1,643,167 - Sama_1354 0.46 -1.7 1,643,170 + Sama_1354 0.47 +0.1 1,643,204 + Sama_1354 0.54 +2.1 1,643,241 + Sama_1354 0.61 +0.1 1,643,302 + Sama_1354 0.74 +0.1 1,643,302 + Sama_1354 0.74 +0.1 1,643,410 + +3.1 1,643,431 + +1.9 1,643,431 + -0.5 1,643,432 - +2.0 1,643,432 - +0.3 1,643,432 - -0.9 1,643,432 - -0.2 1,643,435 + +0.1 1,643,447 + +1.3 1,643,455 + -0.3 1,643,475 - +1.4 1,643,548 + +1.9 1,643,602 - -2.0 1,643,796 + +0.9 1,643,797 - +0.1 1,643,797 - -1.0 1,643,798 + +1.1 1,643,798 + -1.2 1,643,798 + -2.3 1,643,798 + -2.0 1,643,798 + +0.4 1,643,799 - -1.7 1,643,799 - +2.1 1,643,799 - +0.8 1,643,850 + +2.1
Or see this region's nucleotide sequence